Role of the plant-specific endoplasmic reticulum stress-inducible gene TIN1 in the formation of pollen surface structure in Arabidopsis thaliana

Handle URI:
http://hdl.handle.net/10754/562003
Title:
Role of the plant-specific endoplasmic reticulum stress-inducible gene TIN1 in the formation of pollen surface structure in Arabidopsis thaliana
Authors:
Iwata, Yuji; Nishino, Tsuneyo; Iwano, Megumi; Takayama, Seiji; Koizumi, Nozomu
Abstract:
Accumulation of unfolded proteins in the endoplasmic reticulum (ER) of eukaryotic cells triggers the transcriptional activation of ER-resident molecular chaperones and folding enzymes to maintain cellular homeostasis. This process is known as the ER stress response or the unfolded protein response. We have identified tunicamycin induced 1 (TIN1), a plant-specific ER stress-inducible Arabidopsis thaliana gene. The TIN1 protein is localized in the ER; however, its molecular function has yet to be clarified. In this study, we performed functional analysis of TIN1 in planta. RT-PCR analysis showed that TIN1 is highly expressed in pollen. Analysis using the β-glucuronidase reporter gene demonstrated that the TIN1 promoter is active throughout pollen development, peaking at the time of flowering and in an ovule of an open flower. Although a T-DNA insertion mutant of TIN1 grows normally under ambient laboratory conditions, abnormal pollen surface morphology was observed under a scanning electron microscope. Based on the current and previous observations, a possible physiological function of TIN1 during pollen development is discussed. © 2012 The Japanese Society for Plant Cell and Molecular Biology.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division
Publisher:
Japanese Society for Plant Cell and Molecular Biology
Journal:
Plant Biotechnology
Issue Date:
2012
DOI:
10.5511/plantbiotechnology.11.1228a
Type:
Article
ISSN:
13424580
Sponsors:
We would like to thank GABI-Kat and TAIR for the T-DNA insertion mutant and the gene annotation data, respectively. This work was supported by Ministry of Education, Culture, Sports, Science, and Technology of Japan, Grant-in-Aid for Scientific Research 20380188 to N.K.
Appears in Collections:
Articles; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorIwata, Yujien
dc.contributor.authorNishino, Tsuneyoen
dc.contributor.authorIwano, Megumien
dc.contributor.authorTakayama, Seijien
dc.contributor.authorKoizumi, Nozomuen
dc.date.accessioned2015-08-03T09:36:01Zen
dc.date.available2015-08-03T09:36:01Zen
dc.date.issued2012en
dc.identifier.issn13424580en
dc.identifier.doi10.5511/plantbiotechnology.11.1228aen
dc.identifier.urihttp://hdl.handle.net/10754/562003en
dc.description.abstractAccumulation of unfolded proteins in the endoplasmic reticulum (ER) of eukaryotic cells triggers the transcriptional activation of ER-resident molecular chaperones and folding enzymes to maintain cellular homeostasis. This process is known as the ER stress response or the unfolded protein response. We have identified tunicamycin induced 1 (TIN1), a plant-specific ER stress-inducible Arabidopsis thaliana gene. The TIN1 protein is localized in the ER; however, its molecular function has yet to be clarified. In this study, we performed functional analysis of TIN1 in planta. RT-PCR analysis showed that TIN1 is highly expressed in pollen. Analysis using the β-glucuronidase reporter gene demonstrated that the TIN1 promoter is active throughout pollen development, peaking at the time of flowering and in an ovule of an open flower. Although a T-DNA insertion mutant of TIN1 grows normally under ambient laboratory conditions, abnormal pollen surface morphology was observed under a scanning electron microscope. Based on the current and previous observations, a possible physiological function of TIN1 during pollen development is discussed. © 2012 The Japanese Society for Plant Cell and Molecular Biology.en
dc.description.sponsorshipWe would like to thank GABI-Kat and TAIR for the T-DNA insertion mutant and the gene annotation data, respectively. This work was supported by Ministry of Education, Culture, Sports, Science, and Technology of Japan, Grant-in-Aid for Scientific Research 20380188 to N.K.en
dc.publisherJapanese Society for Plant Cell and Molecular Biologyen
dc.subjectArabidopsis thalianaen
dc.subjectEndoplasmic reticulumen
dc.subjectMolecular chaperoneen
dc.subjectPollenen
dc.subjectUnfolded protein responseen
dc.titleRole of the plant-specific endoplasmic reticulum stress-inducible gene TIN1 in the formation of pollen surface structure in Arabidopsis thalianaen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalPlant Biotechnologyen
dc.contributor.institutionHuck Institutes of the Life Sciences, Pennsylvania State University, 211 Wartik Laboratory, University Park, PA 16802, United Statesen
dc.contributor.institutionGraduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Nara 630-0192, Japanen
dc.contributor.institutionGraduate School of Life and Environmental Sciences, Osaka Prefecture University, Sakai, Osaka 599-8531, Japanen
kaust.authorIwata, Yujien
All Items in KAUST are protected by copyright, with all rights reserved, unless otherwise indicated.