Experimental and theoretical evaluation of nanodiamonds as pH triggered drug carriers

Handle URI:
http://hdl.handle.net/10754/561993
Title:
Experimental and theoretical evaluation of nanodiamonds as pH triggered drug carriers
Authors:
Yan, Jingjing; Guo, Yong; Altawashi, Azza; Moosa, Basem ( 0000-0002-2350-4100 ) ; Lecommandoux, Sébastien; Khashab, Niveen M. ( 0000-0003-2728-0666 )
Abstract:
Nanodiamond (ND) and its derivatives have been widely used for drug, protein and gene delivery. Herein, experimental and theoretical methods have been combined to investigate the effect of pH on the delivery of doxorubicin (DOX) from fluorescein labeled NDs (Fc-NDs). In the endosomal recycling process, the nanoparticle will pass from mildly acidic vesicle to pH ≈ 4.8; thus, it is important to investigate DOX release from NDs at different pH values. Fc-NDs released DOX dramatically under acidic conditions, while an increase in the DOX loading efficiency (up to 6.4 wt%) was observed under basic conditions. Further theoretical calculations suggest that H + weakens the electrostatistic interaction between ND surface carboxyl groups and DOX amino groups, and the interaction energies at pH < 7, pH 7 and pH > 7 are 10.4 kcal mol -1, 25.0 kcal mol -1 and 27.0 kcal mol -1 respectively. Cellular imaging experiments show that Fc-NDs are readily ingested by breast adenocarcinoma (BA) cells and cell viability tests prove that they can be utilized as a safe drug delivery vehicle. Furthermore, pH triggered DOX release has been tested in vitro (pH 7.4 and pH 4.83) in breast adenocarcinoma (BA) cells. © The Royal Society of Chemistry and the Centre National de la Recherche Scientifique 2012.
KAUST Department:
Advanced Membranes and Porous Materials Research Center; Smart Hybrid Materials (SHMs) lab; Biological and Environmental Sciences and Engineering (BESE) Division; Physical Sciences and Engineering (PSE) Division; Chemical Science Program; Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
Publisher:
Royal Society of Chemistry (RSC)
Journal:
New Journal of Chemistry
Issue Date:
2012
DOI:
10.1039/c2nj40226b
Type:
Article
ISSN:
11440546
Sponsors:
We thank financial support provided by King Abdullah University of Science and Technology (KAUST).
Appears in Collections:
Articles; Advanced Membranes and Porous Materials Research Center; Physical Sciences and Engineering (PSE) Division; Controlled Release and Delivery Laboratory; Chemical Science Program; Biological and Environmental Sciences and Engineering (BESE) Division; Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorYan, Jingjingen
dc.contributor.authorGuo, Yongen
dc.contributor.authorAltawashi, Azzaen
dc.contributor.authorMoosa, Basemen
dc.contributor.authorLecommandoux, Sébastienen
dc.contributor.authorKhashab, Niveen M.en
dc.date.accessioned2015-08-03T09:35:48Zen
dc.date.available2015-08-03T09:35:48Zen
dc.date.issued2012en
dc.identifier.issn11440546en
dc.identifier.doi10.1039/c2nj40226ben
dc.identifier.urihttp://hdl.handle.net/10754/561993en
dc.description.abstractNanodiamond (ND) and its derivatives have been widely used for drug, protein and gene delivery. Herein, experimental and theoretical methods have been combined to investigate the effect of pH on the delivery of doxorubicin (DOX) from fluorescein labeled NDs (Fc-NDs). In the endosomal recycling process, the nanoparticle will pass from mildly acidic vesicle to pH ≈ 4.8; thus, it is important to investigate DOX release from NDs at different pH values. Fc-NDs released DOX dramatically under acidic conditions, while an increase in the DOX loading efficiency (up to 6.4 wt%) was observed under basic conditions. Further theoretical calculations suggest that H + weakens the electrostatistic interaction between ND surface carboxyl groups and DOX amino groups, and the interaction energies at pH < 7, pH 7 and pH > 7 are 10.4 kcal mol -1, 25.0 kcal mol -1 and 27.0 kcal mol -1 respectively. Cellular imaging experiments show that Fc-NDs are readily ingested by breast adenocarcinoma (BA) cells and cell viability tests prove that they can be utilized as a safe drug delivery vehicle. Furthermore, pH triggered DOX release has been tested in vitro (pH 7.4 and pH 4.83) in breast adenocarcinoma (BA) cells. © The Royal Society of Chemistry and the Centre National de la Recherche Scientifique 2012.en
dc.description.sponsorshipWe thank financial support provided by King Abdullah University of Science and Technology (KAUST).en
dc.publisherRoyal Society of Chemistry (RSC)en
dc.titleExperimental and theoretical evaluation of nanodiamonds as pH triggered drug carriersen
dc.typeArticleen
dc.contributor.departmentAdvanced Membranes and Porous Materials Research Centeren
dc.contributor.departmentSmart Hybrid Materials (SHMs) laben
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentPhysical Sciences and Engineering (PSE) Divisionen
dc.contributor.departmentChemical Science Programen
dc.contributor.departmentComputer, Electrical and Mathematical Sciences and Engineering (CEMSE) Divisionen
dc.identifier.journalNew Journal of Chemistryen
dc.contributor.institutionUniversité de Bordeaux, IPB-ENSCBP, F-33600 Pessac, Franceen
dc.contributor.institutionCNRS, LCPO, UMR 5629, F-33600 Pessac, Franceen
kaust.authorGuo, Yongen
kaust.authorAltawashi, Azzaen
kaust.authorMoosa, Basemen
kaust.authorKhashab, Niveen M.en
kaust.authorYan, Jingjingen
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