The Arabidopsis thaliana cysteine-rich receptor-like kinase CRK20 modulates host responses to Pseudomonas syringae pv. tomato DC3000 infection

Handle URI:
http://hdl.handle.net/10754/561886
Title:
The Arabidopsis thaliana cysteine-rich receptor-like kinase CRK20 modulates host responses to Pseudomonas syringae pv. tomato DC3000 infection
Authors:
Ederli, Luisa; Madeo, Laura; Calderini, Ornella; Gehring, Christoph A. ( 0000-0003-4355-4591 ) ; Moretti, Chiaraluce; Buonaurio, Roberto; Paolocci, Francesco; Pasqualini, Stefania
Abstract:
In plants, the cysteine-rich repeat kinases (CRKs) are a sub-family of receptor-like protein kinases that contain the DUF26 motif in their extracellular domains. It has been shown that in Arabidopsis thaliana, CRK20 is transcriptionally induced by pathogens, salicylic acid and ozone (O3). However, its role in responses to biotic and abiotic stress remains to be elucidated. To determine the function of CRK20 in such responses, two CRK20 loss-of-function mutants, crk20-1 and crk20-2, were isolated from public collections of Arabidopsis T-DNA tagged lines and examined for responses to O3 and Pseudomonas syringae pv. tomato (Pst) DC3000. crk20-1 and crk20-2 showed similar O3 sensitivities and no differences in the expression of defense genes when compared with the wild-type. However, pathogen growth was significantly reduced, while there were no differences in the induction of salicylic acid related defense genes or salicylic acid accumulation. Furthermore, correlation analysis of CRK20 gene expression suggests that it has a role in the control of H2O and/or nutrient transport. We therefore propose that CRK20 promotes conditions that are favorable for Pst DC3000 growth in Arabidopsis, possibly through the regulation of apoplastic homeostasis, and consequently, of the environment of this biotrophic pathogen. © 2011 Elsevier GmbH.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Bioscience Program; Molecular Signalling Group
Publisher:
Elsevier BV
Journal:
Journal of Plant Physiology
Issue Date:
Oct-2011
DOI:
10.1016/j.jplph.2011.05.018
PubMed ID:
21742407
Type:
Article
ISSN:
01761617
Appears in Collections:
Articles; Bioscience Program; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorEderli, Luisaen
dc.contributor.authorMadeo, Lauraen
dc.contributor.authorCalderini, Ornellaen
dc.contributor.authorGehring, Christoph A.en
dc.contributor.authorMoretti, Chiaraluceen
dc.contributor.authorBuonaurio, Robertoen
dc.contributor.authorPaolocci, Francescoen
dc.contributor.authorPasqualini, Stefaniaen
dc.date.accessioned2015-08-03T09:33:20Zen
dc.date.available2015-08-03T09:33:20Zen
dc.date.issued2011-10en
dc.identifier.issn01761617en
dc.identifier.pmid21742407en
dc.identifier.doi10.1016/j.jplph.2011.05.018en
dc.identifier.urihttp://hdl.handle.net/10754/561886en
dc.description.abstractIn plants, the cysteine-rich repeat kinases (CRKs) are a sub-family of receptor-like protein kinases that contain the DUF26 motif in their extracellular domains. It has been shown that in Arabidopsis thaliana, CRK20 is transcriptionally induced by pathogens, salicylic acid and ozone (O3). However, its role in responses to biotic and abiotic stress remains to be elucidated. To determine the function of CRK20 in such responses, two CRK20 loss-of-function mutants, crk20-1 and crk20-2, were isolated from public collections of Arabidopsis T-DNA tagged lines and examined for responses to O3 and Pseudomonas syringae pv. tomato (Pst) DC3000. crk20-1 and crk20-2 showed similar O3 sensitivities and no differences in the expression of defense genes when compared with the wild-type. However, pathogen growth was significantly reduced, while there were no differences in the induction of salicylic acid related defense genes or salicylic acid accumulation. Furthermore, correlation analysis of CRK20 gene expression suggests that it has a role in the control of H2O and/or nutrient transport. We therefore propose that CRK20 promotes conditions that are favorable for Pst DC3000 growth in Arabidopsis, possibly through the regulation of apoplastic homeostasis, and consequently, of the environment of this biotrophic pathogen. © 2011 Elsevier GmbH.en
dc.publisherElsevier BVen
dc.subjectArabidopsis thalianaen
dc.subjectCRKen
dc.subjectOzoneen
dc.subjectPseudomonas syringae pv. tomato DC3000en
dc.subjectReceptor-like protein kinasesen
dc.titleThe Arabidopsis thaliana cysteine-rich receptor-like kinase CRK20 modulates host responses to Pseudomonas syringae pv. tomato DC3000 infectionen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentBioscience Programen
dc.contributor.departmentMolecular Signalling Groupen
dc.identifier.journalJournal of Plant Physiologyen
dc.contributor.institutionDepartment of Applied Biology, University of Perugia, Borgo XX Giugno, 74, I-06121 Perugia, Italyen
dc.contributor.institutionNational Research Council, Plant Genetics Institute Perugia, Via della Madonna Alta 130, I-06128 Perugia, Italyen
dc.contributor.institutionDepartment of Agricultural and Environmental Sciences, University of Perugia, Borgo XX Giugno, 74, I-06121 Perugia, Italyen
kaust.authorGehring, Christoph A.en
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