The glossyhead1 allele of acc1 reveals a principal role for multidomain acetyl-coenzyme a carboxylase in the biosynthesis of cuticular waxes by Arabidopsis

Handle URI:
http://hdl.handle.net/10754/561878
Title:
The glossyhead1 allele of acc1 reveals a principal role for multidomain acetyl-coenzyme a carboxylase in the biosynthesis of cuticular waxes by Arabidopsis
Authors:
Lu, Shiyou; Zhao, Huayan; Parsons, Eugene P.; Xu, Changcheng; Kosma, Dylan K.; Xu, Xiaojing; Chao, Daiyin; Lohrey, Gregory T.; Bangarusamy, Dhinoth Kumar; Wang, Guangchao; Bressan, Ray Anthony; Jenks, Matthew A.
Abstract:
A novel mutant of Arabidopsis (Arabidopsis thaliana), having highly glossy inflorescence stems, postgenital fusion in floral organs, and reduced fertility, was isolated from an ethyl methanesulfonate-mutagenized population and designated glossyhead1 (gsd1). The gsd1 locus was mapped to chromosome 1, and the causal gene was identified as a new allele of Acetyl-Coenzyme A Carboxylase1 (ACC1), a gene encoding the main enzyme in cytosolic malonyl-coenzyme A synthesis. This, to our knowledge, is the first mutant allele of ACC1 that does not cause lethality at the seed or early germination stage, allowing for the first time a detailed analysis of ACC1 function in mature tissues. Broad lipid profiling of mature gsd1 organs revealed a primary role for ACC1 in the biosynthesis of the very-long-chain fatty acids (C 20:0 or longer) associated with cuticular waxes and triacylglycerols. Unexpectedly, transcriptome analysis revealed that gsd1 has limited impact on any lipid metabolic networks but instead has a large effect on environmental stress-responsive pathways, especially senescence and ethylene synthesis determinants, indicating a possible role for the cytosolic malonyl-coenzyme A-derived lipids in stress response signaling. © 2011 American Society of Plant Biologists. All Rights Reserved.
KAUST Department:
Center for Desert Agriculture; Biological and Environmental Sciences and Engineering (BESE) Division; Bioscience Program; Core Labs
Publisher:
American Society of Plant Biologists (ASPB)
Journal:
PLANT PHYSIOLOGY
Issue Date:
23-Sep-2011
DOI:
10.1104/pp.111.185132
PubMed ID:
21949210
PubMed Central ID:
PMC3252135
Type:
Article
ISSN:
00320889
Sponsors:
We are grateful to Dr. Masao Tasaka (Nara Institute of Science and Technology) for providing emb22 and acc1-3 seeds. We also thank Debra Sherman and Chia-Ping Huang of the Purdue University Electron Microscopy Center for support.
Additional Links:
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3252135
Appears in Collections:
Articles; Bioscience Program; Center for Desert Agriculture; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorLu, Shiyouen
dc.contributor.authorZhao, Huayanen
dc.contributor.authorParsons, Eugene P.en
dc.contributor.authorXu, Changchengen
dc.contributor.authorKosma, Dylan K.en
dc.contributor.authorXu, Xiaojingen
dc.contributor.authorChao, Daiyinen
dc.contributor.authorLohrey, Gregory T.en
dc.contributor.authorBangarusamy, Dhinoth Kumaren
dc.contributor.authorWang, Guangchaoen
dc.contributor.authorBressan, Ray Anthonyen
dc.contributor.authorJenks, Matthew A.en
dc.date.accessioned2015-08-03T09:33:07Zen
dc.date.available2015-08-03T09:33:07Zen
dc.date.issued2011-09-23en
dc.identifier.issn00320889en
dc.identifier.pmid21949210en
dc.identifier.doi10.1104/pp.111.185132en
dc.identifier.urihttp://hdl.handle.net/10754/561878en
dc.description.abstractA novel mutant of Arabidopsis (Arabidopsis thaliana), having highly glossy inflorescence stems, postgenital fusion in floral organs, and reduced fertility, was isolated from an ethyl methanesulfonate-mutagenized population and designated glossyhead1 (gsd1). The gsd1 locus was mapped to chromosome 1, and the causal gene was identified as a new allele of Acetyl-Coenzyme A Carboxylase1 (ACC1), a gene encoding the main enzyme in cytosolic malonyl-coenzyme A synthesis. This, to our knowledge, is the first mutant allele of ACC1 that does not cause lethality at the seed or early germination stage, allowing for the first time a detailed analysis of ACC1 function in mature tissues. Broad lipid profiling of mature gsd1 organs revealed a primary role for ACC1 in the biosynthesis of the very-long-chain fatty acids (C 20:0 or longer) associated with cuticular waxes and triacylglycerols. Unexpectedly, transcriptome analysis revealed that gsd1 has limited impact on any lipid metabolic networks but instead has a large effect on environmental stress-responsive pathways, especially senescence and ethylene synthesis determinants, indicating a possible role for the cytosolic malonyl-coenzyme A-derived lipids in stress response signaling. © 2011 American Society of Plant Biologists. All Rights Reserved.en
dc.description.sponsorshipWe are grateful to Dr. Masao Tasaka (Nara Institute of Science and Technology) for providing emb22 and acc1-3 seeds. We also thank Debra Sherman and Chia-Ping Huang of the Purdue University Electron Microscopy Center for support.en
dc.publisherAmerican Society of Plant Biologists (ASPB)en
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3252135en
dc.titleThe glossyhead1 allele of acc1 reveals a principal role for multidomain acetyl-coenzyme a carboxylase in the biosynthesis of cuticular waxes by Arabidopsisen
dc.typeArticleen
dc.contributor.departmentCenter for Desert Agricultureen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentBioscience Programen
dc.contributor.departmentCore Labsen
dc.identifier.journalPLANT PHYSIOLOGYen
dc.identifier.pmcidPMC3252135en
dc.contributor.institutionDepartment of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907-2054, United Statesen
dc.contributor.institutionBiology Department, Brookhaven National Laboratory, Upton NY 11973-5000, United Statesen
dc.contributor.institutionDepartment of Plant Biology, Michigan State University, East Lansing MI 48824, United Statesen
dc.contributor.institutionUnited States Arid Land Agricultural Research Center, United States Department of Agriculture-Agricultural Research Service, Maricopa AZ 85138, United Statesen
kaust.authorLu, Shiyouen
kaust.authorZhao, Huayanen
kaust.authorBangarusamy, Dhinoth Kumaren
kaust.authorWang, Guangchaoen
kaust.authorBressan, Ray Anthonyen

Related articles on PubMed

All Items in KAUST are protected by copyright, with all rights reserved, unless otherwise indicated.