Regeneration in Jatropha curcas: Factors affecting the efficiency of in vitro regeneration

Handle URI:
http://hdl.handle.net/10754/561809
Title:
Regeneration in Jatropha curcas: Factors affecting the efficiency of in vitro regeneration
Authors:
Sharma, Sweta K.; Kumar, Nitish Chandramohana; Reddy, Muppala P.
Abstract:
Factors influencing in vitro regeneration through direct shoot bud induction from hypocotyl explants of Jatropha curcas were studied in the present investigation. Regeneration in J. curcas was found to be genotype dependent and out of four toxic and one non-toxic genotype studied, non-toxic was least responsive. The best results irrespective of genotype were obtained on the medium containing 0.5mgL-1 TDZ (Thidiazuron) and in vitro hypocotyl explants were observed to have higher regeneration efficiency as compared to ex vitro explant in both toxic and non-toxic genotypes. Adventitious shoot buds could be induced from the distal end of explants in all the genotypes. The number of shoot buds formed and not the number of explants responding to TDZ treatment were significantly affected by the position of the explant on the seedling axis. Explants from younger seedlings (≤15 days) were still juvenile and formed callus easily, whereas the regeneration response declined with increase in age of seedlings after 30 days. Transient reduction of Ca2+ concentrations to 0.22gL-1 in the germination medium increased the number of responding explants.Induced shoot buds, upon transfer to MS medium containing 2mgL-1 Kn (Kinetin) and 1mgL-1 BAP (6-benzylamino purine) elongated. These elongated shoots were further proliferated on MS medium supplemented with 1.5mgL-1 IAA (indole-3-acetic acid) and 0.5mgL-1 BAP and 3.01-3.91cm elongation was achieved after 6 weeks. No genotype specific variance in shoot elongation was observed among the toxic genotypes except the CSMCRI-JC2, which showed reduced response. And for proliferation among the toxic genotypes, CSMCRI-JC4 showed highest number of shoots formed. Among the rest, no significant differences were observed. The elongated shoot could be rooted by pulse treatment on half-strength MS medium supplemented with 2% sucrose, 3mgL-1 IBA (indole-3-butyric acid), 1mgL-1 IAA, 1mgL-1 NAA (α-naphthalene acetic acid) and subsequent transfer on 0.25mgL-1 activated charcoal medium. The rooted plants could be established in soil with more than 90% success. No significant differences were observed in rooting of shoots in the different toxic genotypes. However, rooting response was reduced in non-toxic genotype as compared to toxic genotypes. © 2011 Elsevier B.V.
KAUST Department:
Center for Desert Agriculture
Publisher:
Elsevier
Journal:
Industrial Crops and Products
Issue Date:
Jul-2011
DOI:
10.1016/j.indcrop.2011.02.017
Type:
Article
ISSN:
09266690
Sponsors:
Authors are thankful to K.G. Vijay Anand for his help. We are grateful to Dr. P.K. Ghosh Director, CSMCRI for his encouragement and Centre for Scientific and Industrial Research, New Delhi, India for financial support (SRF) to pursue this work. The authors gratefully acknowledge Prof. K. Becker, Department of Aquaculture Systems and Animal Nutrition, University of Hohenheim, Stuttgart, Germany for providing Mexican non-toxic J. curcas seeds.
Appears in Collections:
Articles; Center for Desert Agriculture

Full metadata record

DC FieldValue Language
dc.contributor.authorSharma, Sweta K.en
dc.contributor.authorKumar, Nitish Chandramohanaen
dc.contributor.authorReddy, Muppala P.en
dc.date.accessioned2015-08-03T09:05:06Zen
dc.date.available2015-08-03T09:05:06Zen
dc.date.issued2011-07en
dc.identifier.issn09266690en
dc.identifier.doi10.1016/j.indcrop.2011.02.017en
dc.identifier.urihttp://hdl.handle.net/10754/561809en
dc.description.abstractFactors influencing in vitro regeneration through direct shoot bud induction from hypocotyl explants of Jatropha curcas were studied in the present investigation. Regeneration in J. curcas was found to be genotype dependent and out of four toxic and one non-toxic genotype studied, non-toxic was least responsive. The best results irrespective of genotype were obtained on the medium containing 0.5mgL-1 TDZ (Thidiazuron) and in vitro hypocotyl explants were observed to have higher regeneration efficiency as compared to ex vitro explant in both toxic and non-toxic genotypes. Adventitious shoot buds could be induced from the distal end of explants in all the genotypes. The number of shoot buds formed and not the number of explants responding to TDZ treatment were significantly affected by the position of the explant on the seedling axis. Explants from younger seedlings (≤15 days) were still juvenile and formed callus easily, whereas the regeneration response declined with increase in age of seedlings after 30 days. Transient reduction of Ca2+ concentrations to 0.22gL-1 in the germination medium increased the number of responding explants.Induced shoot buds, upon transfer to MS medium containing 2mgL-1 Kn (Kinetin) and 1mgL-1 BAP (6-benzylamino purine) elongated. These elongated shoots were further proliferated on MS medium supplemented with 1.5mgL-1 IAA (indole-3-acetic acid) and 0.5mgL-1 BAP and 3.01-3.91cm elongation was achieved after 6 weeks. No genotype specific variance in shoot elongation was observed among the toxic genotypes except the CSMCRI-JC2, which showed reduced response. And for proliferation among the toxic genotypes, CSMCRI-JC4 showed highest number of shoots formed. Among the rest, no significant differences were observed. The elongated shoot could be rooted by pulse treatment on half-strength MS medium supplemented with 2% sucrose, 3mgL-1 IBA (indole-3-butyric acid), 1mgL-1 IAA, 1mgL-1 NAA (α-naphthalene acetic acid) and subsequent transfer on 0.25mgL-1 activated charcoal medium. The rooted plants could be established in soil with more than 90% success. No significant differences were observed in rooting of shoots in the different toxic genotypes. However, rooting response was reduced in non-toxic genotype as compared to toxic genotypes. © 2011 Elsevier B.V.en
dc.description.sponsorshipAuthors are thankful to K.G. Vijay Anand for his help. We are grateful to Dr. P.K. Ghosh Director, CSMCRI for his encouragement and Centre for Scientific and Industrial Research, New Delhi, India for financial support (SRF) to pursue this work. The authors gratefully acknowledge Prof. K. Becker, Department of Aquaculture Systems and Animal Nutrition, University of Hohenheim, Stuttgart, Germany for providing Mexican non-toxic J. curcas seeds.en
dc.publisherElsevieren
dc.subjectAgeen
dc.subjectGenotypeen
dc.subjectHypocotylen
dc.subjectJatropha curcasen
dc.subjectRegenerationen
dc.titleRegeneration in Jatropha curcas: Factors affecting the efficiency of in vitro regenerationen
dc.typeArticleen
dc.contributor.departmentCenter for Desert Agricultureen
dc.identifier.journalIndustrial Crops and Productsen
dc.contributor.institutionDiscipline of Wasteland Research, Central Salt and Marine Chemical Research Institute (CSIR), G-B Marg, Bhavnagar 364002, Indiaen
kaust.authorReddy, Muppala P.en
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