Assessment of genetic stability in micropropagules of Jatropha curcas genotypes by RAPD and AFLP analysis

Handle URI:
http://hdl.handle.net/10754/561808
Title:
Assessment of genetic stability in micropropagules of Jatropha curcas genotypes by RAPD and AFLP analysis
Authors:
Sharma, Sweta K.; Pamidimarri, D. V N N Sudheer; Vijay Anand, K. G.; Reddy, Muppala P.
Abstract:
Jatropha curcas (Euphorbiaceae), a drought resistant non edible oil yielding plant, has acquired significant importance as an alternative renewable energy source. Low and inconsistent yields found in field plantations prompted for identification of high yielding clones and their large scale multiplication by vegetative propagation to obtain true to type plants. In the current investigation plantlets of J. curcas generated by axillary bud proliferation (micropropagation) using nodal segments obtained from selected high yielding genotypes were assessed for their genetic stability using Randomly Amplified Polymorphic DNA (RAPD) and Amplified Fragment Length Polymorphism (AFLP) analyses. For RAPD analysis, 21 out of 52 arbitrary decamer primers screened gave clear reproducible bands. In the micropropagated plantlets obtained from the 2nd sub-culture, 4 out of a total of 177 bands scored were polymorphic, but in the 8th and 16th sub-cultures (culture cycle) no polymorphisms were detected. AFLP analysis revealed 0.63%, 0% and 0% polymorphism in the 2nd, 8th and 16th generations, respectively. When different genotypes, viz. IC 56557 16, IC 56557 34 and IC 56557 13, were assessed by AFLP, 0%, 0.31% and 0.47% polymorphisms were found, respectively, indicating a difference in genetic stability among the different genotypes. To the best of our knowledge this is the first report on assessment of genetic stability of micropropagated plantlets in J. curcas and suggests that axillary shoot proliferation can safely be used as an efficient micropropagation method for mass propagation of J. curcas. © 2011 Elsevier B.V.
KAUST Department:
Center for Desert Agriculture
Publisher:
Elsevier BV
Journal:
Industrial Crops and Products
Issue Date:
Jul-2011
DOI:
10.1016/j.indcrop.2011.03.008
Type:
Article
ISSN:
09266690
Sponsors:
We are grateful to Dr. P.K. Ghosh Director, CSMCRI, for his encouragement and Council of Scientific and Industrial Research (CSIR) New Delhi, India for financial support to pursue this work.
Appears in Collections:
Articles; Center for Desert Agriculture

Full metadata record

DC FieldValue Language
dc.contributor.authorSharma, Sweta K.en
dc.contributor.authorPamidimarri, D. V N N Sudheeren
dc.contributor.authorVijay Anand, K. G.en
dc.contributor.authorReddy, Muppala P.en
dc.date.accessioned2015-08-03T09:05:05Zen
dc.date.available2015-08-03T09:05:05Zen
dc.date.issued2011-07en
dc.identifier.issn09266690en
dc.identifier.doi10.1016/j.indcrop.2011.03.008en
dc.identifier.urihttp://hdl.handle.net/10754/561808en
dc.description.abstractJatropha curcas (Euphorbiaceae), a drought resistant non edible oil yielding plant, has acquired significant importance as an alternative renewable energy source. Low and inconsistent yields found in field plantations prompted for identification of high yielding clones and their large scale multiplication by vegetative propagation to obtain true to type plants. In the current investigation plantlets of J. curcas generated by axillary bud proliferation (micropropagation) using nodal segments obtained from selected high yielding genotypes were assessed for their genetic stability using Randomly Amplified Polymorphic DNA (RAPD) and Amplified Fragment Length Polymorphism (AFLP) analyses. For RAPD analysis, 21 out of 52 arbitrary decamer primers screened gave clear reproducible bands. In the micropropagated plantlets obtained from the 2nd sub-culture, 4 out of a total of 177 bands scored were polymorphic, but in the 8th and 16th sub-cultures (culture cycle) no polymorphisms were detected. AFLP analysis revealed 0.63%, 0% and 0% polymorphism in the 2nd, 8th and 16th generations, respectively. When different genotypes, viz. IC 56557 16, IC 56557 34 and IC 56557 13, were assessed by AFLP, 0%, 0.31% and 0.47% polymorphisms were found, respectively, indicating a difference in genetic stability among the different genotypes. To the best of our knowledge this is the first report on assessment of genetic stability of micropropagated plantlets in J. curcas and suggests that axillary shoot proliferation can safely be used as an efficient micropropagation method for mass propagation of J. curcas. © 2011 Elsevier B.V.en
dc.description.sponsorshipWe are grateful to Dr. P.K. Ghosh Director, CSMCRI, for his encouragement and Council of Scientific and Industrial Research (CSIR) New Delhi, India for financial support to pursue this work.en
dc.publisherElsevier BVen
dc.subjectAFLPen
dc.subjectGenetic stabilityen
dc.subjectJatropha curcasen
dc.subjectMicropropagationen
dc.subjectRAPDen
dc.subjectSomaclonal variationsen
dc.titleAssessment of genetic stability in micropropagules of Jatropha curcas genotypes by RAPD and AFLP analysisen
dc.typeArticleen
dc.contributor.departmentCenter for Desert Agricultureen
dc.identifier.journalIndustrial Crops and Productsen
dc.contributor.institutionDiscipline of Wasteland Research, Central Salt and Marine Chemical Research Institute (CSIR), G-B Marg., Bhavnagar 364002, Indiaen
kaust.authorReddy, Muppala P.en
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