Characterization of inhibitor(s) of β-glucuronidase enzyme activity in GUS-transgenic wheat

Handle URI:
http://hdl.handle.net/10754/561804
Title:
Characterization of inhibitor(s) of β-glucuronidase enzyme activity in GUS-transgenic wheat
Authors:
Ramadan, Ahmed M Ali; Eissa, Hala F.; El-Domyati, Fotouh M.; Saleh, Osama Mesilhy; Ibrahim, Nasser E.; Salama, M. I.; Mahfouz, Magdy M. ( 0000-0002-0616-6365 ) ; Bahieldin, Ahmed M.
Abstract:
The uidA gene, encoding for β-glucuronidase (GUS), is the most frequently used reporter gene in plants. As a reporter enzyme, GUS can be assayed both qualitatively and quantitatively. In wheat, there are numerous reports of failure in detecting GUS enzyme activity in tissues of transgenic plants, while other reports have suggested presence of β-glucuronidase inhibitor(s) in wheat tissues. In the present study, we show that the β-glucuronidase enzyme activity is not only tissue-specific but also genotype-dependent. Our data demonstrate that the glucuronic acid could be the candidate inhibitor for β-glucuronidase enzyme activity in wheat leaves and roots. It should be noted that the assays to detect β-glucuronidase enzyme activity in wheat should be interpreted carefully. Based on the data of our present study, we recommend studying the chemical pathways, the unintended effects and the possible loss-of-function of any candidate transgene prior to transformation experiments. © 2011 Springer Science+Business Media B.V.
KAUST Department:
Center for Desert Agriculture; Bioscience Program; Biological and Environmental Sciences and Engineering (BESE) Division
Publisher:
Springer Verlag
Journal:
Plant Cell, Tissue and Organ Culture
Issue Date:
26-Jun-2011
DOI:
10.1007/s11240-011-9988-5
Type:
Article
ISSN:
01676857
Appears in Collections:
Articles; Bioscience Program; Center for Desert Agriculture; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorRamadan, Ahmed M Alien
dc.contributor.authorEissa, Hala F.en
dc.contributor.authorEl-Domyati, Fotouh M.en
dc.contributor.authorSaleh, Osama Mesilhyen
dc.contributor.authorIbrahim, Nasser E.en
dc.contributor.authorSalama, M. I.en
dc.contributor.authorMahfouz, Magdy M.en
dc.contributor.authorBahieldin, Ahmed M.en
dc.date.accessioned2015-08-03T09:04:59Zen
dc.date.available2015-08-03T09:04:59Zen
dc.date.issued2011-06-26en
dc.identifier.issn01676857en
dc.identifier.doi10.1007/s11240-011-9988-5en
dc.identifier.urihttp://hdl.handle.net/10754/561804en
dc.description.abstractThe uidA gene, encoding for β-glucuronidase (GUS), is the most frequently used reporter gene in plants. As a reporter enzyme, GUS can be assayed both qualitatively and quantitatively. In wheat, there are numerous reports of failure in detecting GUS enzyme activity in tissues of transgenic plants, while other reports have suggested presence of β-glucuronidase inhibitor(s) in wheat tissues. In the present study, we show that the β-glucuronidase enzyme activity is not only tissue-specific but also genotype-dependent. Our data demonstrate that the glucuronic acid could be the candidate inhibitor for β-glucuronidase enzyme activity in wheat leaves and roots. It should be noted that the assays to detect β-glucuronidase enzyme activity in wheat should be interpreted carefully. Based on the data of our present study, we recommend studying the chemical pathways, the unintended effects and the possible loss-of-function of any candidate transgene prior to transformation experiments. © 2011 Springer Science+Business Media B.V.en
dc.publisherSpringer Verlagen
dc.subjectβ-glucuronidase enzymeen
dc.subjectd-glucuronic aciden
dc.subjectFluorogenic assaysen
dc.subjectGUSen
dc.subjectHistochemical assayen
dc.subjectl-saccharic acid 1,4 lactoneen
dc.subjectWheaten
dc.titleCharacterization of inhibitor(s) of β-glucuronidase enzyme activity in GUS-transgenic wheaten
dc.typeArticleen
dc.contributor.departmentCenter for Desert Agricultureen
dc.contributor.departmentBioscience Programen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalPlant Cell, Tissue and Organ Cultureen
dc.contributor.institutionAgricultural Genetic Engineering Research Institute (AGERI), ARC, Giza, Egypten
dc.contributor.institutionFaculty of Biotechnology, Misr University for Science and Technology (MUST), 6 October City, Egypten
dc.contributor.institutionBiological Science Department, Faculty of Science, King Abdulaziz Univsity, P.O. Box 9028, Jeddah 21413, Saudi Arabiaen
dc.contributor.institutionDepartment of Biotechnology, Faculty of Applied Medical Science, Taif University, Turrabah, Saudi Arabiaen
dc.contributor.institutionGenetic Engineering and Biotechnology Research Institute, Minufiya University, Sadat Branch, Sadat City, Egypten
kaust.authorMahfouz, Magdy M.en
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