The pro-angiogenic properties of multi-functional bioactive glass composite scaffolds

Handle URI:
http://hdl.handle.net/10754/561783
Title:
The pro-angiogenic properties of multi-functional bioactive glass composite scaffolds
Authors:
Gerhardt, Lutz Christian; Widdows, Kate L.; Erol, Melek M.; Burch, Charles W.; Sanz-Herrera, José A.; Ochoa, Ignacio; Stämpfli, Rolf; Roqan, Iman S. ( 0000-0001-7442-4330 ) ; Gabe, Simon M.; Ansari, Tahera I.; Boccaccini, Aldo R.
Abstract:
The angiogenic properties of micron-sized (m-BG) and nano-sized (n-BG) bioactive glass (BG) filled poly(D,L lactide) (PDLLA) composites were investigated. On the basis of cell culture work investigating the secretion of vascular endothelial growth factor (VEGF) by human fibroblasts in contact with composite films (0, 5, 10, 20 wt %), porous 3D composite scaffolds, optimised with respect to the BG filler content capable of inducing angiogenic response, were produced. The in vivo vascularisation of the scaffolds was studied in a rat animal model and quantified using stereological analyses. The prepared scaffolds had high porosities (81-93%), permeability (k = 5.4-8.6 × 10-9 m2) and compressive strength values (0.4-1.6 MPa) all in the range of trabecular bone. On composite films containing 20 wt % m-BG or n-BG, human fibroblasts produced 5 times higher VEGF than on pure PDLLA films. After 8 weeks of implantation, m-BG and n-BG containing scaffolds were well-infiltrated with newly formed tissue and demonstrated higher vascularisation and percentage blood vessel to tissue (11.6-15.1%) than PDLLA scaffolds (8.5%). This work thus shows potential for the regeneration of hard-soft tissue defects and increased bone formation arising from enhanced vascularisation of the construct. © 2011 Elsevier Ltd.
KAUST Department:
Physical Sciences and Engineering (PSE) Division; Materials Science and Engineering Program; Semiconductor and Material Spectroscopy (SMS) Laboratory
Publisher:
Elsevier
Journal:
Biomaterials
Issue Date:
Jun-2011
DOI:
10.1016/j.biomaterials.2011.02.032
PubMed ID:
21411138
Type:
Article
ISSN:
01429612
Sponsors:
The authors acknowledge the financial support of King Abdullah University of Science and Technology (KAUST). Special thanks are due to Dr. L Serra (Roche, Germany), Dr. J. Lackey (Insight Biotechnology, UK), C. Akufo-Addo (LGC Standards, UK), Dr. M. Ardakani, E. Ware, R. Chater (all Imperial College London) for technical support and helpful discussions. The authors also acknowledge Prof. W. Stark and D. Mohn (ETH Zurich) for providing the nano-sized BG used.
Appears in Collections:
Articles; Physical Sciences and Engineering (PSE) Division; Materials Science and Engineering Program

Full metadata record

DC FieldValue Language
dc.contributor.authorGerhardt, Lutz Christianen
dc.contributor.authorWiddows, Kate L.en
dc.contributor.authorErol, Melek M.en
dc.contributor.authorBurch, Charles W.en
dc.contributor.authorSanz-Herrera, José A.en
dc.contributor.authorOchoa, Ignacioen
dc.contributor.authorStämpfli, Rolfen
dc.contributor.authorRoqan, Iman S.en
dc.contributor.authorGabe, Simon M.en
dc.contributor.authorAnsari, Tahera I.en
dc.contributor.authorBoccaccini, Aldo R.en
dc.date.accessioned2015-08-03T09:04:31Zen
dc.date.available2015-08-03T09:04:31Zen
dc.date.issued2011-06en
dc.identifier.issn01429612en
dc.identifier.pmid21411138en
dc.identifier.doi10.1016/j.biomaterials.2011.02.032en
dc.identifier.urihttp://hdl.handle.net/10754/561783en
dc.description.abstractThe angiogenic properties of micron-sized (m-BG) and nano-sized (n-BG) bioactive glass (BG) filled poly(D,L lactide) (PDLLA) composites were investigated. On the basis of cell culture work investigating the secretion of vascular endothelial growth factor (VEGF) by human fibroblasts in contact with composite films (0, 5, 10, 20 wt %), porous 3D composite scaffolds, optimised with respect to the BG filler content capable of inducing angiogenic response, were produced. The in vivo vascularisation of the scaffolds was studied in a rat animal model and quantified using stereological analyses. The prepared scaffolds had high porosities (81-93%), permeability (k = 5.4-8.6 × 10-9 m2) and compressive strength values (0.4-1.6 MPa) all in the range of trabecular bone. On composite films containing 20 wt % m-BG or n-BG, human fibroblasts produced 5 times higher VEGF than on pure PDLLA films. After 8 weeks of implantation, m-BG and n-BG containing scaffolds were well-infiltrated with newly formed tissue and demonstrated higher vascularisation and percentage blood vessel to tissue (11.6-15.1%) than PDLLA scaffolds (8.5%). This work thus shows potential for the regeneration of hard-soft tissue defects and increased bone formation arising from enhanced vascularisation of the construct. © 2011 Elsevier Ltd.en
dc.description.sponsorshipThe authors acknowledge the financial support of King Abdullah University of Science and Technology (KAUST). Special thanks are due to Dr. L Serra (Roche, Germany), Dr. J. Lackey (Insight Biotechnology, UK), C. Akufo-Addo (LGC Standards, UK), Dr. M. Ardakani, E. Ware, R. Chater (all Imperial College London) for technical support and helpful discussions. The authors also acknowledge Prof. W. Stark and D. Mohn (ETH Zurich) for providing the nano-sized BG used.en
dc.publisherElsevieren
dc.subjectAngiogenesisen
dc.subjectBioactive glassen
dc.subjectBoneen
dc.subjectComposite scaffolden
dc.subjectVascular endothelial growth factoren
dc.subjectVascularisationen
dc.titleThe pro-angiogenic properties of multi-functional bioactive glass composite scaffoldsen
dc.typeArticleen
dc.contributor.departmentPhysical Sciences and Engineering (PSE) Divisionen
dc.contributor.departmentMaterials Science and Engineering Programen
dc.contributor.departmentSemiconductor and Material Spectroscopy (SMS) Laboratoryen
dc.identifier.journalBiomaterialsen
dc.contributor.institutionUniv Erlangen Nurnberg, Inst Biomat, D-91058 Erlangen, Germanyen
dc.contributor.institutionUniv London Imperial Coll Sci Technol & Med, Dept Mat, London SW7 2BP, Englanden
dc.contributor.institutionNorthwick Pk Inst Med Res, Dept Surg Res, Harrow HA1 3UJ, Middx, Englanden
dc.contributor.institutionIstanbul Tech Univ, Dept Chem Engn, TR-34469 Istanbul, Turkeyen
dc.contributor.institutionUniv Seville, Sch Engn, Seville 41092, Spainen
dc.contributor.institutionUniv Zaragoza, Dept Mech Engn, Zaragoza 50018, Spainen
dc.contributor.institutionSwiss Fed Labs Mat Sci & Technol, EMPA, Lab Protect & Physiol, CH-9014 St Gallen, Switzerlanden
dc.contributor.institutionSt Marks Hosp, Dept Gastroenterol Endoscopy, Harrow HA1 3UJ, Middx, Englanden
kaust.authorRoqan, Iman S.en

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