Label-free, electrochemical detection of methicillin-resistant staphylococcus aureus DNA with reduced graphene oxide-modified electrodes

Handle URI:
http://hdl.handle.net/10754/561763
Title:
Label-free, electrochemical detection of methicillin-resistant staphylococcus aureus DNA with reduced graphene oxide-modified electrodes
Authors:
Wang, Zhijuan; Zhang, Juan; Chen, Peng; Zhou, Xiaozhu; Yang, Yanli; Wu, Shixin; Niu, Li; Han, Yu ( 0000-0003-1462-1118 ) ; Wang, Lianhui; Chen, Peng; Boey, Freddy; Zhang, Qichun; Liedberg, Bo Gunnar; Zhang, Hua
Abstract:
Reduced graphene oxide (rGO)-modified glassy carbon electrode is used to detect the methicillin-resistant Staphylococcus aureus (MRSA) DNA by using electrochemical impedance spectroscopy. Our experiments confirm that ssDNA, before and after hybridization with target DNA, are successfully anchored on the rGO surface. After the probe DNA, pre-adsorbed on rGO electrode, hybridizes with target DNA, the measured impedance increases dramatically. It provides a new method to detect DNA with high sensitivity (10-13M, i.e., 100 fM) and selectivity. © 2011 Elsevier B.V.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Physical Sciences and Engineering (PSE) Division; Chemical Science Program; Advanced Membranes and Porous Materials Research Center; Nanostructured Functional Materials (NFM) laboratory
Publisher:
Elsevier BV
Journal:
Biosensors and Bioelectronics
Issue Date:
May-2011
DOI:
10.1016/j.bios.2011.03.002
Type:
Article
ISSN:
09565663
Sponsors:
This work was supported by the AcRF Tier 2 (ARC 10/10, no. MOE2010-T2-1-060) from MOE, POC (S08/1-82563404) from EDB, CRP (NRF-CRP2-2007-01) from NRF, an A*STAR SERC Grant (no. 092 101 0064) from A*STAR,New Initiative Fund FY 2010 (M58120031) from NTU, and the Centre for Biomimetic Sensor Science at NTU in Singapore. P.C. thanks the financial support from A*STAR SERC (grant no. 072 101 0020) in Singapore. B.L. also acknowledges support from the Swedish Research Council and the NanoSence program the Foundation for Strategic Research.
Appears in Collections:
Articles; Advanced Membranes and Porous Materials Research Center; Physical Sciences and Engineering (PSE) Division; Chemical Science Program; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorWang, Zhijuanen
dc.contributor.authorZhang, Juanen
dc.contributor.authorChen, Pengen
dc.contributor.authorZhou, Xiaozhuen
dc.contributor.authorYang, Yanlien
dc.contributor.authorWu, Shixinen
dc.contributor.authorNiu, Lien
dc.contributor.authorHan, Yuen
dc.contributor.authorWang, Lianhuien
dc.contributor.authorChen, Pengen
dc.contributor.authorBoey, Freddyen
dc.contributor.authorZhang, Qichunen
dc.contributor.authorLiedberg, Bo Gunnaren
dc.contributor.authorZhang, Huaen
dc.date.accessioned2015-08-03T09:04:04Zen
dc.date.available2015-08-03T09:04:04Zen
dc.date.issued2011-05en
dc.identifier.issn09565663en
dc.identifier.doi10.1016/j.bios.2011.03.002en
dc.identifier.urihttp://hdl.handle.net/10754/561763en
dc.description.abstractReduced graphene oxide (rGO)-modified glassy carbon electrode is used to detect the methicillin-resistant Staphylococcus aureus (MRSA) DNA by using electrochemical impedance spectroscopy. Our experiments confirm that ssDNA, before and after hybridization with target DNA, are successfully anchored on the rGO surface. After the probe DNA, pre-adsorbed on rGO electrode, hybridizes with target DNA, the measured impedance increases dramatically. It provides a new method to detect DNA with high sensitivity (10-13M, i.e., 100 fM) and selectivity. © 2011 Elsevier B.V.en
dc.description.sponsorshipThis work was supported by the AcRF Tier 2 (ARC 10/10, no. MOE2010-T2-1-060) from MOE, POC (S08/1-82563404) from EDB, CRP (NRF-CRP2-2007-01) from NRF, an A*STAR SERC Grant (no. 092 101 0064) from A*STAR,New Initiative Fund FY 2010 (M58120031) from NTU, and the Centre for Biomimetic Sensor Science at NTU in Singapore. P.C. thanks the financial support from A*STAR SERC (grant no. 072 101 0020) in Singapore. B.L. also acknowledges support from the Swedish Research Council and the NanoSence program the Foundation for Strategic Research.en
dc.publisherElsevier BVen
dc.subjectDNAen
dc.subjectGrapheneen
dc.subjectImpedance spectroscopyen
dc.subjectLabel-free detectionen
dc.subjectMethicillin-resistant Staphylococcus aureusen
dc.subjectReduced graphene oxideen
dc.titleLabel-free, electrochemical detection of methicillin-resistant staphylococcus aureus DNA with reduced graphene oxide-modified electrodesen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentPhysical Sciences and Engineering (PSE) Divisionen
dc.contributor.departmentChemical Science Programen
dc.contributor.departmentAdvanced Membranes and Porous Materials Research Centeren
dc.contributor.departmentNanostructured Functional Materials (NFM) laboratoryen
dc.identifier.journalBiosensors and Bioelectronicsen
dc.contributor.institutionSchool of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, SGP 639798, Singaporeen
dc.contributor.institutionCenter for Biomimetic Sensor Science, Nanyang Technological University, 50 Nanyang Drive, SGP 637553, Singaporeen
dc.contributor.institutionState Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, Chinaen
dc.contributor.institutionLaboratory of Advanced Materials, Fudan University, Shanghai 200438, Chinaen
dc.contributor.institutionSchool of Chemical and Biomedical Engineering, Nanyang Technological University, 70 Nanyang Drive, SGP 637457, Singaporeen
kaust.authorHan, Yuen
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