Plant natriuretic peptides are apoplastic and paracrine stress response molecules

Handle URI:
http://hdl.handle.net/10754/561750
Title:
Plant natriuretic peptides are apoplastic and paracrine stress response molecules
Authors:
Wang, Yuhua; Gehring, Christoph A. ( 0000-0003-4355-4591 ) ; Irving, Helen R.
Abstract:
Higher plants contain biologically active proteins that are recognized by antibodies against human atrial natriuretic peptide (ANP). We identified and isolated two Arabidopsis thaliana immunoreactive plant natriuretic peptide (PNP)-encoding genes, AtPNP-A and AtPNP-B, which are distantly related members of the expansin superfamily and have a role in the regulation of homeostasis in abiotic and biotic stresses, and have shown that AtPNP-A modulates the effects of ABA on stomata. Arabidopsis PNP (PNP-A) is mainly expressed in leaf mesophyll cells, and in protoplast assays we demonstrate that it is secreted using AtPNP-A:green fluorescent protein (GFP) reporter constructs and flow cytometry. Transient reporter assays provide evidence that AtPNP-A expression is enhanced by heat, osmotica and salt, and that AtPNP-A itself can enhance its own expression, thereby generating a response signature diagnostic for paracrine action and potentially also autocrine effects. Expression of native AtPNP-A is enhanced by osmotica and transiently by salt. Although AtPNP-A expression is induced by salt and osmotica, ABA does not significantly modulate AtPNP-A levels nor does recombinant AtPNP-A affect reporter expression of the ABA-responsive RD29A gene. Together, these results provide experimental evidence that AtPNP-A is stress responsive, secreted into the apoplastic space and can enhance its own expression. Furthermore, our findings support the idea that AtPNP-A, together with ABA, is an important component in complex plant stress responses and that, much like in animals, peptide signaling molecules can create diverse and modular signals essential for growth, development and defense under rapidly changing environmental conditions. © 2011 The Author.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Bioscience Program; Molecular Signalling Group
Publisher:
Oxford University Press (OUP)
Journal:
Plant and Cell Physiology
Issue Date:
7-Apr-2011
DOI:
10.1093/pcp/pcr036
PubMed ID:
21478192
Type:
Article
ISSN:
00320781
Sponsors:
This work was supported by the Australian Research Council [Discovery project funding scheme (DP0557561, DP0878194)]; Australian Postgraduate Award [to Y.H.W.].
Appears in Collections:
Articles; Bioscience Program; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorWang, Yuhuaen
dc.contributor.authorGehring, Christoph A.en
dc.contributor.authorIrving, Helen R.en
dc.date.accessioned2015-08-03T09:03:45Zen
dc.date.available2015-08-03T09:03:45Zen
dc.date.issued2011-04-07en
dc.identifier.issn00320781en
dc.identifier.pmid21478192en
dc.identifier.doi10.1093/pcp/pcr036en
dc.identifier.urihttp://hdl.handle.net/10754/561750en
dc.description.abstractHigher plants contain biologically active proteins that are recognized by antibodies against human atrial natriuretic peptide (ANP). We identified and isolated two Arabidopsis thaliana immunoreactive plant natriuretic peptide (PNP)-encoding genes, AtPNP-A and AtPNP-B, which are distantly related members of the expansin superfamily and have a role in the regulation of homeostasis in abiotic and biotic stresses, and have shown that AtPNP-A modulates the effects of ABA on stomata. Arabidopsis PNP (PNP-A) is mainly expressed in leaf mesophyll cells, and in protoplast assays we demonstrate that it is secreted using AtPNP-A:green fluorescent protein (GFP) reporter constructs and flow cytometry. Transient reporter assays provide evidence that AtPNP-A expression is enhanced by heat, osmotica and salt, and that AtPNP-A itself can enhance its own expression, thereby generating a response signature diagnostic for paracrine action and potentially also autocrine effects. Expression of native AtPNP-A is enhanced by osmotica and transiently by salt. Although AtPNP-A expression is induced by salt and osmotica, ABA does not significantly modulate AtPNP-A levels nor does recombinant AtPNP-A affect reporter expression of the ABA-responsive RD29A gene. Together, these results provide experimental evidence that AtPNP-A is stress responsive, secreted into the apoplastic space and can enhance its own expression. Furthermore, our findings support the idea that AtPNP-A, together with ABA, is an important component in complex plant stress responses and that, much like in animals, peptide signaling molecules can create diverse and modular signals essential for growth, development and defense under rapidly changing environmental conditions. © 2011 The Author.en
dc.description.sponsorshipThis work was supported by the Australian Research Council [Discovery project funding scheme (DP0557561, DP0878194)]; Australian Postgraduate Award [to Y.H.W.].en
dc.publisherOxford University Press (OUP)en
dc.subjectABAen
dc.subjectAbiotic stressen
dc.subjectArabidopsis thalianaen
dc.subjectDroughten
dc.subjectPlant natriuretic peptidesen
dc.subjectSalinityen
dc.titlePlant natriuretic peptides are apoplastic and paracrine stress response moleculesen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentBioscience Programen
dc.contributor.departmentMolecular Signalling Groupen
dc.identifier.journalPlant and Cell Physiologyen
dc.contributor.institutionMonash Institute of Pharmaceutical Sciences, Monash University 381 Royal Parade, Parkville, VIC 3052, Australiaen
dc.contributor.institutionDepartment of Biotechnology, University of the Western Cape, Private Bag X17, Bellville 7535, South Africaen
kaust.authorGehring, Christoph A.en

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