TALE nucleases and next generation GM crops.

Handle URI:
http://hdl.handle.net/10754/561749
Title:
TALE nucleases and next generation GM crops.
Authors:
Mahfouz, Magdy M. ( 0000-0002-0616-6365 ) ; Li, Lixin
Abstract:
Site-specific and adaptable DNA binding domains are essential modules to develop genome engineering technologies for crop improvement. Transcription activator-like effectors (TALEs) proteins are used to provide a highly specific and adaptable DNA binding modules. TALE chimeric nucleases (TALENs) were used to generate site-specific double strand breaks (DSBs) in vitro and in yeast, Caenorhabditis elegans, mammalian and plant cells. The genomic DSBs can be generated at predefined and user-selected loci and repaired by either the non-homologous end joining (NHEJ) or homology dependent repair (HDR). Thus, TALENs can be used to achieve site-specific gene addition, stacking, deletion or inactivation. TALE-based genome engineering tools should be powerful to develop new agricultural biotechnology approaches for crop improvement. Here, we discuss the recent research and the potential applications of TALENs to accelerate the generation of genomic variants through targeted mutagenesis and to produce a non-transgenic GM crops with the desired phenotype.
KAUST Department:
Center for Desert Agriculture; Bioscience Program; Biological and Environmental Sciences and Engineering (BESE) Division
Publisher:
Taylor & Francis
Journal:
GM crops
Issue Date:
1-Apr-2011
DOI:
10.4161/gmcr.2.2.17254
PubMed ID:
21865862
Type:
Article
ISSN:
19382006
Appears in Collections:
Articles; Bioscience Program; Center for Desert Agriculture; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorMahfouz, Magdy M.en
dc.contributor.authorLi, Lixinen
dc.date.accessioned2015-08-03T09:03:44Zen
dc.date.available2015-08-03T09:03:44Zen
dc.date.issued2011-04-01en
dc.identifier.issn19382006en
dc.identifier.pmid21865862en
dc.identifier.doi10.4161/gmcr.2.2.17254en
dc.identifier.urihttp://hdl.handle.net/10754/561749en
dc.description.abstractSite-specific and adaptable DNA binding domains are essential modules to develop genome engineering technologies for crop improvement. Transcription activator-like effectors (TALEs) proteins are used to provide a highly specific and adaptable DNA binding modules. TALE chimeric nucleases (TALENs) were used to generate site-specific double strand breaks (DSBs) in vitro and in yeast, Caenorhabditis elegans, mammalian and plant cells. The genomic DSBs can be generated at predefined and user-selected loci and repaired by either the non-homologous end joining (NHEJ) or homology dependent repair (HDR). Thus, TALENs can be used to achieve site-specific gene addition, stacking, deletion or inactivation. TALE-based genome engineering tools should be powerful to develop new agricultural biotechnology approaches for crop improvement. Here, we discuss the recent research and the potential applications of TALENs to accelerate the generation of genomic variants through targeted mutagenesis and to produce a non-transgenic GM crops with the desired phenotype.en
dc.publisherTaylor & Francisen
dc.titleTALE nucleases and next generation GM crops.en
dc.typeArticleen
dc.contributor.departmentCenter for Desert Agricultureen
dc.contributor.departmentBioscience Programen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalGM cropsen
kaust.authorMahfouz, Magdy M.en

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