NKS1, Na+- and K+-sensitive 1, regulates ion homeostasis in an SOS-independent pathway in Arabidopsis

Handle URI:
http://hdl.handle.net/10754/561741
Title:
NKS1, Na+- and K+-sensitive 1, regulates ion homeostasis in an SOS-independent pathway in Arabidopsis
Authors:
Choi, Wonkyun; Baek, Dongwon; Oh, Dongha; Park, Jiyoung; Hong, Hyewon; Kim, Woeyeon; Bohnert, Hans Jürgen; Bressan, Ray Anthony; Park, Hyeongcheol; Yun, Daejin
Abstract:
An Arabidopsis thaliana mutant, nks1-1, exhibiting enhanced sensitivity to NaCl was identified in a screen of a T-DNA insertion population in the genetic background of Col-0 gl1 sos3-1. Analysis of the genome sequence in the region flanking the T-DNA left border indicated two closely linked mutations in the gene encoded at locus At4g30996. A second allele, nks1-2, was obtained from the Arabidopsis Biological Resource Center. NKS1 mRNA was detected in all parts of wild-type plants but was not detected in plants of either mutant, indicating inactivation by the mutations. Both mutations in NKS1 were associated with increased sensitivity to NaCl and KCl, but not to LiCl or mannitol. NaCl sensitivity was associated with nks1 mutations in Arabidopsis lines expressing either wild type or alleles of SOS1, SOS2 or SOS3. The NaCl-sensitive phenotype of the nks1-2 mutant was complemented by expression of a full-length NKS1 allele from the CaMV35S promoter. When grown in medium containing NaCl, nks1 mutants accumulated more Na+ than wild type and K +/Na+ homeostasis was perturbed. It is proposed NKS1, a plant-specific gene encoding a 19 kDa endomembrane-localized protein of unknown function, is part of an ion homeostasis regulation pathway that is independent of the SOS pathway. © 2011 Elsevier Ltd. All rights reserved.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division
Publisher:
Elsevier
Journal:
Phytochemistry
Issue Date:
Apr-2011
DOI:
10.1016/j.phytochem.2010.12.005
PubMed ID:
21227472
Type:
Article
ISSN:
00319422
Sponsors:
This work was supported by Grants from the World Class University Program (Grant No. R32-10148) funded by the Ministry of Education, Science and Technology and the Biogreen 21 Program (Grant No. 20070301034030) of the Rural Development Administration in Korea. W.C. is supported by a scholarship from the Brain Korea 21 program of the Ministry of Education, Science and Technology.
Appears in Collections:
Articles; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorChoi, Wonkyunen
dc.contributor.authorBaek, Dongwonen
dc.contributor.authorOh, Donghaen
dc.contributor.authorPark, Jiyoungen
dc.contributor.authorHong, Hyewonen
dc.contributor.authorKim, Woeyeonen
dc.contributor.authorBohnert, Hans Jürgenen
dc.contributor.authorBressan, Ray Anthonyen
dc.contributor.authorPark, Hyeongcheolen
dc.contributor.authorYun, Daejinen
dc.date.accessioned2015-08-03T09:03:34Zen
dc.date.available2015-08-03T09:03:34Zen
dc.date.issued2011-04en
dc.identifier.issn00319422en
dc.identifier.pmid21227472en
dc.identifier.doi10.1016/j.phytochem.2010.12.005en
dc.identifier.urihttp://hdl.handle.net/10754/561741en
dc.description.abstractAn Arabidopsis thaliana mutant, nks1-1, exhibiting enhanced sensitivity to NaCl was identified in a screen of a T-DNA insertion population in the genetic background of Col-0 gl1 sos3-1. Analysis of the genome sequence in the region flanking the T-DNA left border indicated two closely linked mutations in the gene encoded at locus At4g30996. A second allele, nks1-2, was obtained from the Arabidopsis Biological Resource Center. NKS1 mRNA was detected in all parts of wild-type plants but was not detected in plants of either mutant, indicating inactivation by the mutations. Both mutations in NKS1 were associated with increased sensitivity to NaCl and KCl, but not to LiCl or mannitol. NaCl sensitivity was associated with nks1 mutations in Arabidopsis lines expressing either wild type or alleles of SOS1, SOS2 or SOS3. The NaCl-sensitive phenotype of the nks1-2 mutant was complemented by expression of a full-length NKS1 allele from the CaMV35S promoter. When grown in medium containing NaCl, nks1 mutants accumulated more Na+ than wild type and K +/Na+ homeostasis was perturbed. It is proposed NKS1, a plant-specific gene encoding a 19 kDa endomembrane-localized protein of unknown function, is part of an ion homeostasis regulation pathway that is independent of the SOS pathway. © 2011 Elsevier Ltd. All rights reserved.en
dc.description.sponsorshipThis work was supported by Grants from the World Class University Program (Grant No. R32-10148) funded by the Ministry of Education, Science and Technology and the Biogreen 21 Program (Grant No. 20070301034030) of the Rural Development Administration in Korea. W.C. is supported by a scholarship from the Brain Korea 21 program of the Ministry of Education, Science and Technology.en
dc.publisherElsevieren
dc.subjectArabidopsis thalianaen
dc.subjectCruciferaeen
dc.subjectEndomembrane systemen
dc.subjectIon homeostasisen
dc.subjectNaCl stressen
dc.subjectNKS1en
dc.subjectSalt stressen
dc.titleNKS1, Na+- and K+-sensitive 1, regulates ion homeostasis in an SOS-independent pathway in Arabidopsisen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalPhytochemistryen
dc.contributor.institutionDivision of Applied Life Science (BK21 Program), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju 660-701, South Koreaen
dc.contributor.institutionDivision of Applied Life Science (BK21 Program), Environmental Biotechnology National Core Research Center, Gyeongsang National University, Jinju 660-701, South Koreaen
dc.contributor.institutionDepartment of Plant Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, United Statesen
dc.contributor.institutionDepartment of Crop Sciences, University of Illinois at Urbana-Champaign, Urbana, IL 61801, United Statesen
dc.contributor.institutionCenter for Plant Environmental Stress Physiology, Purdue University, West Lafayette, IN 47907, United Statesen
kaust.authorBressan, Ray Anthonyen

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