Shoot-supplied ammonium targets the root auxin influx carrier AUX1 and inhibits lateral root emergence in Arabidopsis

Handle URI:
http://hdl.handle.net/10754/561735
Title:
Shoot-supplied ammonium targets the root auxin influx carrier AUX1 and inhibits lateral root emergence in Arabidopsis
Authors:
Li, Baohai; Li, Qing; Su, Yanhua; Chen, Hao; Xiong, Liming ( 0000-0001-8099-0806 ) ; Mi, Guohua; Kronzucker, Herbert J.; Shi, Weiming
Abstract:
Deposition of ammonium (NH4 +) from the atmosphere is a substantial environmental problem. While toxicity resulting from root exposure to NH4 + is well studied, little is known about how shoot-supplied ammonium (SSA) affects root growth. In this study, we show that SSA significantly affects lateral root (LR) development. We show that SSA inhibits lateral root primordium (LRP) emergence, but not LRP initiation, resulting in significantly impaired LR number. We show that the inhibition is independent of abscisic acid (ABA) signalling and sucrose uptake in shoots but relates to the auxin response in roots. Expression analyses of an auxin-responsive reporter, DR5:GUS, and direct assays of auxin transport demonstrated that SSA inhibits root acropetal (rootward) auxin transport while not affecting basipetal (shootward) transport or auxin sensitivity of root cells. Mutant analyses indicated that the auxin influx carrier AUX1, but not the auxin efflux carriers PIN-FORMED (PIN)1 or PIN2, is required for this inhibition of LRP emergence and the observed auxin response. We found that AUX1 expression was modulated by SSA in vascular tissues rather than LR cap cells in roots. Taken together, our results suggest that SSA inhibits LRP emergence in Arabidopsis by interfering with AUX1-dependent auxin transport from shoot to root. © 2011 Blackwell Publishing Ltd.
KAUST Department:
Center for Desert Agriculture; Biological and Environmental Sciences and Engineering (BESE) Division; Bioscience Program; Plant Stress Genomics Research Lab
Publisher:
Wiley
Journal:
Plant, Cell and Environment
Issue Date:
24-Mar-2011
DOI:
10.1111/j.1365-3040.2011.02295.x
PubMed ID:
21342208
Type:
Article
ISSN:
01407791
Sponsors:
We thank Professors Malcolm Bennett (University of Nottingham), Ben Scheres (Utrecht University) and Tom Guilfoyle (University of Missouri) for providing the transgenic lines of Arabidopsis, and the Arabidopsis Biological Resource Center for the mutant seeds. We are grateful to Malcolm Bennett (University of Nottingham) for invaluable advice during designing and writing of the manuscript. We also thank other members of our team for helpful comments on the manuscript. This work was supported by the National Basic Research Program of China (2007CB109303), the National Natural Science Foundation of China (30771285) and the National Sciences and Engineering Research Council of Canada (NSERC, Discovery Grant 217277-2009).
Appears in Collections:
Articles; Bioscience Program; Center for Desert Agriculture; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorLi, Baohaien
dc.contributor.authorLi, Qingen
dc.contributor.authorSu, Yanhuaen
dc.contributor.authorChen, Haoen
dc.contributor.authorXiong, Limingen
dc.contributor.authorMi, Guohuaen
dc.contributor.authorKronzucker, Herbert J.en
dc.contributor.authorShi, Weimingen
dc.date.accessioned2015-08-03T09:03:25Zen
dc.date.available2015-08-03T09:03:25Zen
dc.date.issued2011-03-24en
dc.identifier.issn01407791en
dc.identifier.pmid21342208en
dc.identifier.doi10.1111/j.1365-3040.2011.02295.xen
dc.identifier.urihttp://hdl.handle.net/10754/561735en
dc.description.abstractDeposition of ammonium (NH4 +) from the atmosphere is a substantial environmental problem. While toxicity resulting from root exposure to NH4 + is well studied, little is known about how shoot-supplied ammonium (SSA) affects root growth. In this study, we show that SSA significantly affects lateral root (LR) development. We show that SSA inhibits lateral root primordium (LRP) emergence, but not LRP initiation, resulting in significantly impaired LR number. We show that the inhibition is independent of abscisic acid (ABA) signalling and sucrose uptake in shoots but relates to the auxin response in roots. Expression analyses of an auxin-responsive reporter, DR5:GUS, and direct assays of auxin transport demonstrated that SSA inhibits root acropetal (rootward) auxin transport while not affecting basipetal (shootward) transport or auxin sensitivity of root cells. Mutant analyses indicated that the auxin influx carrier AUX1, but not the auxin efflux carriers PIN-FORMED (PIN)1 or PIN2, is required for this inhibition of LRP emergence and the observed auxin response. We found that AUX1 expression was modulated by SSA in vascular tissues rather than LR cap cells in roots. Taken together, our results suggest that SSA inhibits LRP emergence in Arabidopsis by interfering with AUX1-dependent auxin transport from shoot to root. © 2011 Blackwell Publishing Ltd.en
dc.description.sponsorshipWe thank Professors Malcolm Bennett (University of Nottingham), Ben Scheres (Utrecht University) and Tom Guilfoyle (University of Missouri) for providing the transgenic lines of Arabidopsis, and the Arabidopsis Biological Resource Center for the mutant seeds. We are grateful to Malcolm Bennett (University of Nottingham) for invaluable advice during designing and writing of the manuscript. We also thank other members of our team for helpful comments on the manuscript. This work was supported by the National Basic Research Program of China (2007CB109303), the National Natural Science Foundation of China (30771285) and the National Sciences and Engineering Research Council of Canada (NSERC, Discovery Grant 217277-2009).en
dc.publisherWileyen
dc.subjectABAen
dc.subjectAmmonium toxicityen
dc.subjectAuxin transporten
dc.subjectRooten
dc.subjectShoot-derived signalen
dc.titleShoot-supplied ammonium targets the root auxin influx carrier AUX1 and inhibits lateral root emergence in Arabidopsisen
dc.typeArticleen
dc.contributor.departmentCenter for Desert Agricultureen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentBioscience Programen
dc.contributor.departmentPlant Stress Genomics Research Laben
dc.identifier.journalPlant, Cell and Environmenten
dc.contributor.institutionState Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, Chinaen
dc.contributor.institutionGraduate School of Chinese Academy of Science, Beijing 100081, Chinaen
dc.contributor.institutionDonald Danforth Plant Science Center, St. Louis Missouri 63132, United Statesen
dc.contributor.institutionCollege of Resources and Environmental Science, China Agricultural University, Beijing 100094, Chinaen
dc.contributor.institutionDepartment of Biological Sciences, University of Toronto, 1265 Military Trail, Toronto, ON M1C 1A4, Canadaen
kaust.authorXiong, Limingen
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