Design and validation of a highly discriminatory 10-locus Y-chromosome STR multiplex system

Handle URI:
http://hdl.handle.net/10754/561724
Title:
Design and validation of a highly discriminatory 10-locus Y-chromosome STR multiplex system
Authors:
D'Amato, María Eugenia; Bajic, Vladimir B. ( 0000-0001-5435-4750 ) ; Davison, Sean P.
Abstract:
The Y-chromosome STRs (short tandem repeat) markers are routinely utilized in the resolution of forensic casework related to sexual assault. For this, the forensic community has adopted a set of eleven (core) Y-STR that is incorporated in all commercial diagnostic systems. Our previous studies of Y-STR polymorphisms in the South African population identified low levels of diversity and discrimination capacity for many commercial marker sets, determining a limited applicability of these systems to the local population groups. To overcome this shortcoming, we designed a Y-STR 10-plex system that shows higher discriminatory capacity (DC) than available commercial systems. The markers were selected from a population group of 283 individuals with African, European and Asian ancestry genotyped at 45 Y-STRs, applying an optimization based selection procedure to achieve the highest possible DC with the minimal number of markers. The 10-plex was satisfactorily subjected to developmental validation tests following the SWGDAM guidelines and shows potential for its application to genealogical and evolutionary studies. © 2010 Elsevier Ireland Ltd.
KAUST Department:
Computational Bioscience Research Center (CBRC); Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division; Applied Mathematics and Computational Science Program
Publisher:
Elsevier
Journal:
Forensic Science International: Genetics
Issue Date:
Mar-2011
DOI:
10.1016/j.fsigen.2010.08.015
PubMed ID:
20884311
Type:
Article
ISSN:
18724973
Appears in Collections:
Articles; Applied Mathematics and Computational Science Program; Computational Bioscience Research Center (CBRC); Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorD'Amato, María Eugeniaen
dc.contributor.authorBajic, Vladimir B.en
dc.contributor.authorDavison, Sean P.en
dc.date.accessioned2015-08-03T09:03:10Zen
dc.date.available2015-08-03T09:03:10Zen
dc.date.issued2011-03en
dc.identifier.issn18724973en
dc.identifier.pmid20884311en
dc.identifier.doi10.1016/j.fsigen.2010.08.015en
dc.identifier.urihttp://hdl.handle.net/10754/561724en
dc.description.abstractThe Y-chromosome STRs (short tandem repeat) markers are routinely utilized in the resolution of forensic casework related to sexual assault. For this, the forensic community has adopted a set of eleven (core) Y-STR that is incorporated in all commercial diagnostic systems. Our previous studies of Y-STR polymorphisms in the South African population identified low levels of diversity and discrimination capacity for many commercial marker sets, determining a limited applicability of these systems to the local population groups. To overcome this shortcoming, we designed a Y-STR 10-plex system that shows higher discriminatory capacity (DC) than available commercial systems. The markers were selected from a population group of 283 individuals with African, European and Asian ancestry genotyped at 45 Y-STRs, applying an optimization based selection procedure to achieve the highest possible DC with the minimal number of markers. The 10-plex was satisfactorily subjected to developmental validation tests following the SWGDAM guidelines and shows potential for its application to genealogical and evolutionary studies. © 2010 Elsevier Ireland Ltd.en
dc.publisherElsevieren
dc.subjectDevelopmental validationen
dc.subjectForensic geneticsen
dc.subjectMultiplexen
dc.subjectY-STRsen
dc.titleDesign and validation of a highly discriminatory 10-locus Y-chromosome STR multiplex systemen
dc.typeArticleen
dc.contributor.departmentComputational Bioscience Research Center (CBRC)en
dc.contributor.departmentComputer, Electrical and Mathematical Sciences and Engineering (CEMSE) Divisionen
dc.contributor.departmentApplied Mathematics and Computational Science Programen
dc.identifier.journalForensic Science International: Geneticsen
dc.contributor.institutionForensic DNA Lab., University of the Western Cape, Biotechnology Department, Private Bag X17, 7535 Bellville, Cape Town, South Africaen
dc.contributor.institutionSANBI (South African National Bioinformatics Institute), University of the Western Cape, South Africaen
kaust.authorBajic, Vladimir B.en

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