Arabidopsis decuple mutant reveals the importance of SnRK2 kinases in osmotic stress responses in vivo

Handle URI:
http://hdl.handle.net/10754/561701
Title:
Arabidopsis decuple mutant reveals the importance of SnRK2 kinases in osmotic stress responses in vivo
Authors:
Fujii, Hiroaki ( 0000-0002-0013-5891 ) ; Verslues, Paul E.; Zhu, Jian-Kang
Abstract:
Osmotic stress associated with drought or salinity is a major factor that limits plant productivity. Protein kinases in the SNF1-related protein kinase 2 (SnRK2) family are activated by osmotic stress, suggesting that the kinases are involved in osmotic stress signaling. However, due to functional redundancy, their contribution to osmotic stress responses remained unclear. In this report, we constructed an Arabidopsis line carrying mutations in all 10 members of the SnRK2 family. The decuple mutant snrk2.1/2/3/4/5/6/7/8/9/10 grew poorly under hyperosmotic stress conditions but was similar to the wild type in culture media in the absence of osmotic stress. The mutant was also defective in gene regulation and the accumulation of abscisic acid (ABA), proline, and inositol 1,4,5-trisphosphate under osmotic stress. In addition, analysis of mutants defective in the ABA-activated SnRK2s (snrk2.2/3/6) and mutants defective in the rest of the SnRK2s (snrk2.1/4/5/7/8/9/10) revealed that SnRK2s are a merging point of ABA-dependent and -independent pathways for osmotic stress responses. These results demonstrate critical functions of the SnRK2s in mediating osmotic stress signaling and tolerance.
KAUST Department:
Center for Desert Agriculture
Publisher:
National Academy of Sciences
Journal:
Proceedings of the National Academy of Sciences of the United States of America
Issue Date:
10-Jan-2011
DOI:
10.1073/pnas.1018367108
PubMed ID:
21220313
PubMed Central ID:
PMC3029766
Type:
Article
ISSN:
00278424
Sponsors:
We thank the Arabidopsis Biological Resource Center for providing the T-DNA insertion mutants. This work was supported by National Institutes of Health Grant R01GM059138 (to J.-K.Z.).
Additional Links:
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3029766
Appears in Collections:
Articles; Center for Desert Agriculture

Full metadata record

DC FieldValue Language
dc.contributor.authorFujii, Hiroakien
dc.contributor.authorVerslues, Paul E.en
dc.contributor.authorZhu, Jian-Kangen
dc.date.accessioned2015-08-03T09:02:38Zen
dc.date.available2015-08-03T09:02:38Zen
dc.date.issued2011-01-10en
dc.identifier.issn00278424en
dc.identifier.pmid21220313en
dc.identifier.doi10.1073/pnas.1018367108en
dc.identifier.urihttp://hdl.handle.net/10754/561701en
dc.description.abstractOsmotic stress associated with drought or salinity is a major factor that limits plant productivity. Protein kinases in the SNF1-related protein kinase 2 (SnRK2) family are activated by osmotic stress, suggesting that the kinases are involved in osmotic stress signaling. However, due to functional redundancy, their contribution to osmotic stress responses remained unclear. In this report, we constructed an Arabidopsis line carrying mutations in all 10 members of the SnRK2 family. The decuple mutant snrk2.1/2/3/4/5/6/7/8/9/10 grew poorly under hyperosmotic stress conditions but was similar to the wild type in culture media in the absence of osmotic stress. The mutant was also defective in gene regulation and the accumulation of abscisic acid (ABA), proline, and inositol 1,4,5-trisphosphate under osmotic stress. In addition, analysis of mutants defective in the ABA-activated SnRK2s (snrk2.2/3/6) and mutants defective in the rest of the SnRK2s (snrk2.1/4/5/7/8/9/10) revealed that SnRK2s are a merging point of ABA-dependent and -independent pathways for osmotic stress responses. These results demonstrate critical functions of the SnRK2s in mediating osmotic stress signaling and tolerance.en
dc.description.sponsorshipWe thank the Arabidopsis Biological Resource Center for providing the T-DNA insertion mutants. This work was supported by National Institutes of Health Grant R01GM059138 (to J.-K.Z.).en
dc.publisherNational Academy of Sciencesen
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3029766en
dc.subjectAbiotic stressen
dc.subjectOsmosensingen
dc.subjectPhosphorylationen
dc.subjectSignal transductionen
dc.subjectWater deficiten
dc.titleArabidopsis decuple mutant reveals the importance of SnRK2 kinases in osmotic stress responses in vivoen
dc.typeArticleen
dc.contributor.departmentCenter for Desert Agricultureen
dc.identifier.journalProceedings of the National Academy of Sciences of the United States of Americaen
dc.identifier.pmcidPMC3029766en
dc.contributor.institutionDepartment of Botany and Plant Sciences, University of California, Riverside, CA 92521, United Statesen
dc.contributor.institutionDepartment of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907-2010, United Statesen
dc.contributor.institutionInstitute of Plant and Microbial Biology, Academia Sinica, Taipei 11529, Taiwanen
kaust.authorFujii, Hiroakien
kaust.authorZhu, Jian-Kangen

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