Gene function analysis by artificial microRNAs in Physcomitrella patens.

Handle URI:
http://hdl.handle.net/10754/561658
Title:
Gene function analysis by artificial microRNAs in Physcomitrella patens.
Authors:
Khraiwesh, Basel; Fattash, Isam; Arif, Muhammad Asif; Frank, Wolfgang
Abstract:
MicroRNAs (miRNAs) are ~21 nt long small RNAs transcribed from endogenous MIR genes which form precursor RNAs with a characteristic hairpin structure. miRNAs control the expression of cognate target genes by binding to reverse complementary sequences resulting in cleavage or translational inhibition of the target RNA. Artificial miRNAs (amiRNAs) can be generated by exchanging the miRNA/miRNA sequence of endogenous MIR precursor genes, while maintaining the general pattern of matches and mismatches in the foldback. Thus, for functional gene analysis amiRNAs can be designed to target any gene of interest. During the last decade the moss Physcomitrella patens emerged as a model plant for functional gene analysis based on its unique ability to integrate DNA into the nuclear genome by homologous recombination which allows for the generation of targeted gene knockout mutants. In addition to this, we developed a protocol to express amiRNAs in P. patens that has particular advantages over the generation of knockout mutants and might be used to speed up reverse genetics approaches in this model species.
KAUST Department:
Center for Desert Agriculture
Publisher:
Springer Science + Business Media
Journal:
RNAi and Plant Gene Function Analysis
Issue Date:
2011
DOI:
10.1007/978-1-61779-123-9_5
PubMed ID:
21533686
Type:
Article
ISSN:
19406029
Appears in Collections:
Articles; Center for Desert Agriculture

Full metadata record

DC FieldValue Language
dc.contributor.authorKhraiwesh, Baselen
dc.contributor.authorFattash, Isamen
dc.contributor.authorArif, Muhammad Asifen
dc.contributor.authorFrank, Wolfgangen
dc.date.accessioned2015-08-03T09:01:38Zen
dc.date.available2015-08-03T09:01:38Zen
dc.date.issued2011en
dc.identifier.issn19406029en
dc.identifier.pmid21533686en
dc.identifier.doi10.1007/978-1-61779-123-9_5en
dc.identifier.urihttp://hdl.handle.net/10754/561658en
dc.description.abstractMicroRNAs (miRNAs) are ~21 nt long small RNAs transcribed from endogenous MIR genes which form precursor RNAs with a characteristic hairpin structure. miRNAs control the expression of cognate target genes by binding to reverse complementary sequences resulting in cleavage or translational inhibition of the target RNA. Artificial miRNAs (amiRNAs) can be generated by exchanging the miRNA/miRNA sequence of endogenous MIR precursor genes, while maintaining the general pattern of matches and mismatches in the foldback. Thus, for functional gene analysis amiRNAs can be designed to target any gene of interest. During the last decade the moss Physcomitrella patens emerged as a model plant for functional gene analysis based on its unique ability to integrate DNA into the nuclear genome by homologous recombination which allows for the generation of targeted gene knockout mutants. In addition to this, we developed a protocol to express amiRNAs in P. patens that has particular advantages over the generation of knockout mutants and might be used to speed up reverse genetics approaches in this model species.en
dc.publisherSpringer Science + Business Mediaen
dc.titleGene function analysis by artificial microRNAs in Physcomitrella patens.en
dc.typeArticleen
dc.contributor.departmentCenter for Desert Agricultureen
dc.identifier.journalRNAi and Plant Gene Function Analysisen
kaust.authorKhraiwesh, Baselen

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