Pyrosequencing reveals highly diverse and species-specific microbial communities in sponges from the Red Sea

Handle URI:
http://hdl.handle.net/10754/561636
Title:
Pyrosequencing reveals highly diverse and species-specific microbial communities in sponges from the Red Sea
Authors:
Lee, Onon; Wang, Yong; Yang, Jiangke; Lafi, Feras Fawzi; Al-Suwailem, Abdulaziz M.; Qian, Peiyuan
Abstract:
Marine sponges are associated with a remarkable array of microorganisms. Using a tag pyrosequencing technology, this study was the first to investigate in depth the microbial communities associated with three Red Sea sponges, Hyrtios erectus, Stylissa carteri and Xestospongia testudinaria. We revealed highly diverse sponge-associated bacterial communities with up to 1000 microbial operational taxonomic units (OTUs) and richness estimates of up to 2000 species. Altogether, 26 bacterial phyla were detected from the Red Sea sponges, 11 of which were absent from the surrounding sea water and 4 were recorded in sponges for the first time. Up to 100 OTUs with richness estimates of up to 300 archaeal species were revealed from a single sponge species. This is by far the highest archaeal diversity ever recorded for sponges. A non-negligible proportion of unclassified reads was observed in sponges. Our results demonstrated that the sponge-associated microbial communities remained highly consistent in the same sponge species from different locations, although they varied at different degrees among different sponge species. A significant proportion of the tag sequences from the sponges could be assigned to one of the sponge-specific clusters previously defined. In addition, the sponge-associated microbial communities were consistently divergent from those present in the surrounding sea water. Our results suggest that the Red Sea sponges possess highly sponge-specific or even sponge-species-specific microbial communities that are resistant to environmental disturbance, and much of their microbial diversity remains to be explored. © 2011 International Society for Microbial Ecology All rights reserved.
KAUST Department:
KAUST Global Partnership Program; Center for Desert Agriculture; Coastal and Marine Resources Core Lab
Publisher:
Springer Nature
Journal:
The ISME Journal
Issue Date:
18-Nov-2010
DOI:
10.1038/ismej.2010.165
PubMed ID:
21085196
PubMed Central ID:
PMC3105750
Type:
Article
ISSN:
17517362
Sponsors:
We thank HC Chung, YH Wong, M Li and JP Ren from Hong Kong University of Science and Technology (HKUST), D Lau from Baptist University of Hong Kong and the technical team from King Abdullah University of Science and Technology (KAUST) for technical help during sample collection. We also thank Professor Rob von Soest, Zoological Museum, University of Amsterdam, for identification of sponges, and HC Chung, K Cheng and YK Tam, HKUST, for assistance in nutrient analysis. We are grateful to Professor MW Taylor, University of Auckland, for providing an ARB database containing the alignment of all sponge-derived sequences for comparison. This publication is based on the work supported by an award (SA-C0040/UK-C0016) made by KAUST to P-Y Qian.
Additional Links:
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3105750
Appears in Collections:
Articles; Coastal and Marine Resources Core Lab; Center for Desert Agriculture

Full metadata record

DC FieldValue Language
dc.contributor.authorLee, Ononen
dc.contributor.authorWang, Yongen
dc.contributor.authorYang, Jiangkeen
dc.contributor.authorLafi, Feras Fawzien
dc.contributor.authorAl-Suwailem, Abdulaziz M.en
dc.contributor.authorQian, Peiyuanen
dc.date.accessioned2015-08-03T09:01:00Zen
dc.date.available2015-08-03T09:01:00Zen
dc.date.issued2010-11-18en
dc.identifier.issn17517362en
dc.identifier.pmid21085196en
dc.identifier.doi10.1038/ismej.2010.165en
dc.identifier.urihttp://hdl.handle.net/10754/561636en
dc.description.abstractMarine sponges are associated with a remarkable array of microorganisms. Using a tag pyrosequencing technology, this study was the first to investigate in depth the microbial communities associated with three Red Sea sponges, Hyrtios erectus, Stylissa carteri and Xestospongia testudinaria. We revealed highly diverse sponge-associated bacterial communities with up to 1000 microbial operational taxonomic units (OTUs) and richness estimates of up to 2000 species. Altogether, 26 bacterial phyla were detected from the Red Sea sponges, 11 of which were absent from the surrounding sea water and 4 were recorded in sponges for the first time. Up to 100 OTUs with richness estimates of up to 300 archaeal species were revealed from a single sponge species. This is by far the highest archaeal diversity ever recorded for sponges. A non-negligible proportion of unclassified reads was observed in sponges. Our results demonstrated that the sponge-associated microbial communities remained highly consistent in the same sponge species from different locations, although they varied at different degrees among different sponge species. A significant proportion of the tag sequences from the sponges could be assigned to one of the sponge-specific clusters previously defined. In addition, the sponge-associated microbial communities were consistently divergent from those present in the surrounding sea water. Our results suggest that the Red Sea sponges possess highly sponge-specific or even sponge-species-specific microbial communities that are resistant to environmental disturbance, and much of their microbial diversity remains to be explored. © 2011 International Society for Microbial Ecology All rights reserved.en
dc.description.sponsorshipWe thank HC Chung, YH Wong, M Li and JP Ren from Hong Kong University of Science and Technology (HKUST), D Lau from Baptist University of Hong Kong and the technical team from King Abdullah University of Science and Technology (KAUST) for technical help during sample collection. We also thank Professor Rob von Soest, Zoological Museum, University of Amsterdam, for identification of sponges, and HC Chung, K Cheng and YK Tam, HKUST, for assistance in nutrient analysis. We are grateful to Professor MW Taylor, University of Auckland, for providing an ARB database containing the alignment of all sponge-derived sequences for comparison. This publication is based on the work supported by an award (SA-C0040/UK-C0016) made by KAUST to P-Y Qian.en
dc.publisherSpringer Natureen
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3105750en
dc.subjectmicrobial community structureen
dc.subjectpyrosequencingen
dc.subjectspongeen
dc.subjectsponge specificen
dc.titlePyrosequencing reveals highly diverse and species-specific microbial communities in sponges from the Red Seaen
dc.typeArticleen
dc.contributor.departmentKAUST Global Partnership Programen
dc.contributor.departmentCenter for Desert Agricultureen
dc.contributor.departmentCoastal and Marine Resources Core Laben
dc.identifier.journalThe ISME Journalen
dc.identifier.pmcidPMC3105750en
kaust.authorWang, Yongen
kaust.authorLafi, Feras Fawzien
kaust.authorAl-Suwailem, Abdulaziz M.en

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