An unusual alphasatellite associated with monopartite begomoviruses attenuates symptoms and reduces betasatellite accumulation

Handle URI:
http://hdl.handle.net/10754/561635
Title:
An unusual alphasatellite associated with monopartite begomoviruses attenuates symptoms and reduces betasatellite accumulation
Authors:
Idris, Ali; Shahid, Muhammad Shafiq; Briddon, Rob William; Khan, Akhtarjamal; Zhu, Jian-Kang; Brown, Judith K.
Abstract:
The Oman strain of Tomato yellow leaf curl virus (TYLCV-OM) and its associated betasatellite, an isolate of Tomato leaf curl betasatellite (ToLCB), were previously reported from Oman. Here we report the isolation of a second, previously undescribed, begomovirus [Tomato leaf curl Oman virus (ToLCOMV)] and an alphasatellite from that same plant sample. This alphasatellite is closely related (90% shared nucleotide identity) to an unusual DNA-2-type Ageratum yellow vein Singapore alphasatellite (AYVSGA), thus far identified only in Singapore. ToLCOMV was found to have a recombinant genome comprising sequences derived from two extant parents, TYLCV-OM, which is indigenous to Oman, and Papaya leaf curl virus from the Indian subcontinent. All possible combinations of ToLCOMV, TYLCV-OM, ToLCB and AYVSGA were used to agro-inoculate tomato and Nicotiana benthamiana. Infection with ToLCOMV yielded mild leaf-curl symptoms in both hosts; however, plants inoculated with TYLCV-OM developed more severe symptoms. Plants infected with ToLCB in the presence of either helper begomovirus resulted in more severe symptoms. Surprisingly, symptoms in N. benthamiana infected with the alphasatellite together with either of the helper viruses and the betasatellite were attenuated and betasatellite DNA accumulation was substantially reduced. However, in the latter plants no concomitant reduction in the accumulation of helper virus DNA was observed. This is the first example of an attenuation of begomovirus-betasatellite symptoms by this unusual class of alphasatellites. This observation suggests that some DNA-2 alphasatellites encode a pathogenicity determinant that may modulate begomovirus-betasatellite infection by reducing betasatellite DNA accumulation. © 2011 SGM.
KAUST Department:
Center for Desert Agriculture
Publisher:
Society for General Microbiology
Journal:
Journal of General Virology
Issue Date:
17-Nov-2010
DOI:
10.1099/vir.0.025288-0
PubMed ID:
21084498
Type:
Article
ISSN:
00221317
Sponsors:
M. S. S. was supported by a PhD fellowship from the Higher Education Commission (HEC), Government of Pakistan. R. W. B. is supported by the HEC under the 'Foreign Faculty Program'.
Appears in Collections:
Articles; Center for Desert Agriculture

Full metadata record

DC FieldValue Language
dc.contributor.authorIdris, Alien
dc.contributor.authorShahid, Muhammad Shafiqen
dc.contributor.authorBriddon, Rob Williamen
dc.contributor.authorKhan, Akhtarjamalen
dc.contributor.authorZhu, Jian-Kangen
dc.contributor.authorBrown, Judith K.en
dc.date.accessioned2015-08-03T09:00:58Zen
dc.date.available2015-08-03T09:00:58Zen
dc.date.issued2010-11-17en
dc.identifier.issn00221317en
dc.identifier.pmid21084498en
dc.identifier.doi10.1099/vir.0.025288-0en
dc.identifier.urihttp://hdl.handle.net/10754/561635en
dc.description.abstractThe Oman strain of Tomato yellow leaf curl virus (TYLCV-OM) and its associated betasatellite, an isolate of Tomato leaf curl betasatellite (ToLCB), were previously reported from Oman. Here we report the isolation of a second, previously undescribed, begomovirus [Tomato leaf curl Oman virus (ToLCOMV)] and an alphasatellite from that same plant sample. This alphasatellite is closely related (90% shared nucleotide identity) to an unusual DNA-2-type Ageratum yellow vein Singapore alphasatellite (AYVSGA), thus far identified only in Singapore. ToLCOMV was found to have a recombinant genome comprising sequences derived from two extant parents, TYLCV-OM, which is indigenous to Oman, and Papaya leaf curl virus from the Indian subcontinent. All possible combinations of ToLCOMV, TYLCV-OM, ToLCB and AYVSGA were used to agro-inoculate tomato and Nicotiana benthamiana. Infection with ToLCOMV yielded mild leaf-curl symptoms in both hosts; however, plants inoculated with TYLCV-OM developed more severe symptoms. Plants infected with ToLCB in the presence of either helper begomovirus resulted in more severe symptoms. Surprisingly, symptoms in N. benthamiana infected with the alphasatellite together with either of the helper viruses and the betasatellite were attenuated and betasatellite DNA accumulation was substantially reduced. However, in the latter plants no concomitant reduction in the accumulation of helper virus DNA was observed. This is the first example of an attenuation of begomovirus-betasatellite symptoms by this unusual class of alphasatellites. This observation suggests that some DNA-2 alphasatellites encode a pathogenicity determinant that may modulate begomovirus-betasatellite infection by reducing betasatellite DNA accumulation. © 2011 SGM.en
dc.description.sponsorshipM. S. S. was supported by a PhD fellowship from the Higher Education Commission (HEC), Government of Pakistan. R. W. B. is supported by the HEC under the 'Foreign Faculty Program'.en
dc.publisherSociety for General Microbiologyen
dc.titleAn unusual alphasatellite associated with monopartite begomoviruses attenuates symptoms and reduces betasatellite accumulationen
dc.typeArticleen
dc.contributor.departmentCenter for Desert Agricultureen
dc.identifier.journalJournal of General Virologyen
dc.contributor.institutionSchool of Plant Sciences, University of Arizona, Tucson, AZ 85721, United Statesen
dc.contributor.institutionNational Institute for Biotechnology and Genetic Engineering, Jhang Road, Faisalabad, Pakistanen
dc.contributor.institutionDepartment of Crop Sciences, College of Agricultural and Marine Sciences, Sultan Qaboos University, Al-Khod 123, Muscat, Omanen
kaust.authorIdris, Alien
kaust.authorZhu, Jian-Kangen

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