Characterization of opaque2 modifier QTLs and candidate genes in recombinant inbred lines derived from the K0326Y quality protein maize inbred

Handle URI:
http://hdl.handle.net/10754/561634
Title:
Characterization of opaque2 modifier QTLs and candidate genes in recombinant inbred lines derived from the K0326Y quality protein maize inbred
Authors:
Holding, David R.; Hunter, Brenda G.; Klingler, John ( 0000-0003-3951-9734 ) ; Wu, Song; Guo, Xiaomei; Gibbon, Bryan C.; Wu., Rongling; Schulze, Jan Michele; Jung, Rudolf; Larkins, Brian A.
Abstract:
Quality protein maize (QPM) is a high lysine-containing corn that is based on genetic modification of the opaque2 (o2) mutant. In QPM, modifier genes convert the starchy endosperm of o2 to the vitreous phenotype of wild type maize. There are multiple, unlinked o2 modifier loci (Opm) in QPM and their nature and mode of action are unknown. We previously identified seven Opm QTLs and characterized 16 genes that are differentially up-regulated at a significant level in K0326Y QPM, compared to the starchy endosperm mutant W64Ao2. In order to further characterize these Opm QTLs and the genes up-regulated in K0326Y QPM, we created a population of 314 recombinant inbred lines (RILs) from a cross between K0326Y QPM and W64Ao2. The RILs were characterized for three traits associated with endosperm texture: vitreousness, density and hardness. Genetic linkage analysis of the RIL population confirmed three of the previously identified QTLs associated with o2 endosperm modification in K0326Y QPM. Many of the genes up-regulated in K0326Y QPM showed substantially higher levels of expression in vitreous compared with opaque RILs. These included genes associated with the upstream regulation of the ethylene response pathway, and a gene encoding a regulatory subunit of pyrophosphate-dependent fructose-6-phosphate 1-phosphotransferase, an adaptive enzyme of the glycolytic pathway. © 2010 Springer-Verlag.
KAUST Department:
Center for Desert Agriculture
Publisher:
Springer Nature
Journal:
Theoretical and Applied Genetics
Issue Date:
13-Nov-2010
DOI:
10.1007/s00122-010-1486-3
PubMed ID:
21076810
Type:
Article
ISSN:
00405752
Sponsors:
The research described in this manuscript was supported by grants from the USDA (CSREES 2004-35301-14537), DOE (DE-FG02-96ER20242) and Pioneer Hi-Bred to BAL. We thank Roberto Lizzaraga-Guerra for contributing to the creation of the genetic materials that were used in this analysis.
Appears in Collections:
Articles; Center for Desert Agriculture

Full metadata record

DC FieldValue Language
dc.contributor.authorHolding, David R.en
dc.contributor.authorHunter, Brenda G.en
dc.contributor.authorKlingler, Johnen
dc.contributor.authorWu, Songen
dc.contributor.authorGuo, Xiaomeien
dc.contributor.authorGibbon, Bryan C.en
dc.contributor.authorWu., Ronglingen
dc.contributor.authorSchulze, Jan Micheleen
dc.contributor.authorJung, Rudolfen
dc.contributor.authorLarkins, Brian A.en
dc.date.accessioned2015-08-03T09:00:57Zen
dc.date.available2015-08-03T09:00:57Zen
dc.date.issued2010-11-13en
dc.identifier.issn00405752en
dc.identifier.pmid21076810en
dc.identifier.doi10.1007/s00122-010-1486-3en
dc.identifier.urihttp://hdl.handle.net/10754/561634en
dc.description.abstractQuality protein maize (QPM) is a high lysine-containing corn that is based on genetic modification of the opaque2 (o2) mutant. In QPM, modifier genes convert the starchy endosperm of o2 to the vitreous phenotype of wild type maize. There are multiple, unlinked o2 modifier loci (Opm) in QPM and their nature and mode of action are unknown. We previously identified seven Opm QTLs and characterized 16 genes that are differentially up-regulated at a significant level in K0326Y QPM, compared to the starchy endosperm mutant W64Ao2. In order to further characterize these Opm QTLs and the genes up-regulated in K0326Y QPM, we created a population of 314 recombinant inbred lines (RILs) from a cross between K0326Y QPM and W64Ao2. The RILs were characterized for three traits associated with endosperm texture: vitreousness, density and hardness. Genetic linkage analysis of the RIL population confirmed three of the previously identified QTLs associated with o2 endosperm modification in K0326Y QPM. Many of the genes up-regulated in K0326Y QPM showed substantially higher levels of expression in vitreous compared with opaque RILs. These included genes associated with the upstream regulation of the ethylene response pathway, and a gene encoding a regulatory subunit of pyrophosphate-dependent fructose-6-phosphate 1-phosphotransferase, an adaptive enzyme of the glycolytic pathway. © 2010 Springer-Verlag.en
dc.description.sponsorshipThe research described in this manuscript was supported by grants from the USDA (CSREES 2004-35301-14537), DOE (DE-FG02-96ER20242) and Pioneer Hi-Bred to BAL. We thank Roberto Lizzaraga-Guerra for contributing to the creation of the genetic materials that were used in this analysis.en
dc.publisherSpringer Natureen
dc.titleCharacterization of opaque2 modifier QTLs and candidate genes in recombinant inbred lines derived from the K0326Y quality protein maize inbreden
dc.typeArticleen
dc.contributor.departmentCenter for Desert Agricultureen
dc.identifier.journalTheoretical and Applied Geneticsen
dc.contributor.institutionUniv Arizona, Dept Plant Sci, Tucson, AZ 85721 USAen
dc.contributor.institutionUniv Nebraska, Ctr Plant Sci Innovat, Dept Agron & Hort, Lincoln, NE 68588 USAen
dc.contributor.institutionSt Jude Childrens Hosp, Dept Biostat, Memphis, TN 38105 USAen
dc.contributor.institutionBaylor Univ, Dept Biol, Waco, TX 76798 USAen
dc.contributor.institutionPenn State Univ, Ctr Stat Genet, Hershey, PA 17033 USAen
dc.contributor.institutionPioneer HiBred Int Inc, Johnston, IA 50131 USAen
kaust.authorKlingler, Johnen

Related articles on PubMed

All Items in KAUST are protected by copyright, with all rights reserved, unless otherwise indicated.