Cross species amplification ability of novel microsatellites isolated from Jatropha curcas and genetic relationship with sister taxa : Cross species amplification and genetic relationship of Jatropha using novel microsatellites

Handle URI:
http://hdl.handle.net/10754/561625
Title:
Cross species amplification ability of novel microsatellites isolated from Jatropha curcas and genetic relationship with sister taxa : Cross species amplification and genetic relationship of Jatropha using novel microsatellites
Authors:
Pamidimarri, D. V N N Sudheer; Mastan, Shaik G.; Rahman, Hifzur; Ravi Prakash, Ch; Singh, Sweta V.; Reddy, Muppala P.
Abstract:
The present investigation was undertaken with an aim to check the ability of cross species amplification of microsatellite markers isolated from Jatropha curcas-a renewable source of biodiesel to deduce the generic relationship with its six sister taxa (J. glandulifera, J. gossypifolia, J. integerrima, J. multifida, J. podagrica, and J. tanjorensis). Out of the 49 markers checked 31 markers showed cross species amplification in all the species studied. JCDS-30, JCDS-69, JCDS-26, JCMS-13 and JCMS-21 amplified in J. curcas. However, these markers did not show any cross species amplification. Overall percentage of polymorphism (PP) among the species studied was 38% and the mean genetic similarity (GS) was found to be 0.86. The highest PP (24) and least GS (0.76) was found between J. curcas/J. podagrica and J. curcas/J. multifida and least PP (4.44) and highest GS (0.96) was found between J. integerrima/J. tanjorensis. Dendrogram analysis showed good congruence to RAPD and AFLP than nrDNA ITS data reported earlier. The characterized microsatellites will pave way for intraspecies molecular characterization which can be further utilized in species differentiation, molecular identification, characterization of interspecific hybrids, exploitation of genetic resource management and genetic improvement of the species through marker assisted breeding for economically important traits. © 2010 Springer Science+Business Media B.V.
KAUST Department:
Center for Desert Agriculture
Publisher:
Springer Verlag
Journal:
Molecular Biology Reports
Issue Date:
30-Jul-2010
DOI:
10.1007/s11033-010-0241-9
PubMed ID:
20676774
Type:
Article
ISSN:
03014851
Sponsors:
We are thankful to Council for Scientific and Industrial Research, New Delhi, India for its financial support and for research associate fellowship.
Appears in Collections:
Articles; Center for Desert Agriculture

Full metadata record

DC FieldValue Language
dc.contributor.authorPamidimarri, D. V N N Sudheeren
dc.contributor.authorMastan, Shaik G.en
dc.contributor.authorRahman, Hifzuren
dc.contributor.authorRavi Prakash, Chen
dc.contributor.authorSingh, Sweta V.en
dc.contributor.authorReddy, Muppala P.en
dc.date.accessioned2015-08-03T09:00:38Zen
dc.date.available2015-08-03T09:00:38Zen
dc.date.issued2010-07-30en
dc.identifier.issn03014851en
dc.identifier.pmid20676774en
dc.identifier.doi10.1007/s11033-010-0241-9en
dc.identifier.urihttp://hdl.handle.net/10754/561625en
dc.description.abstractThe present investigation was undertaken with an aim to check the ability of cross species amplification of microsatellite markers isolated from Jatropha curcas-a renewable source of biodiesel to deduce the generic relationship with its six sister taxa (J. glandulifera, J. gossypifolia, J. integerrima, J. multifida, J. podagrica, and J. tanjorensis). Out of the 49 markers checked 31 markers showed cross species amplification in all the species studied. JCDS-30, JCDS-69, JCDS-26, JCMS-13 and JCMS-21 amplified in J. curcas. However, these markers did not show any cross species amplification. Overall percentage of polymorphism (PP) among the species studied was 38% and the mean genetic similarity (GS) was found to be 0.86. The highest PP (24) and least GS (0.76) was found between J. curcas/J. podagrica and J. curcas/J. multifida and least PP (4.44) and highest GS (0.96) was found between J. integerrima/J. tanjorensis. Dendrogram analysis showed good congruence to RAPD and AFLP than nrDNA ITS data reported earlier. The characterized microsatellites will pave way for intraspecies molecular characterization which can be further utilized in species differentiation, molecular identification, characterization of interspecific hybrids, exploitation of genetic resource management and genetic improvement of the species through marker assisted breeding for economically important traits. © 2010 Springer Science+Business Media B.V.en
dc.description.sponsorshipWe are thankful to Council for Scientific and Industrial Research, New Delhi, India for its financial support and for research associate fellowship.en
dc.publisherSpringer Verlagen
dc.subjectCross species amplificationen
dc.subjectGenetic relationshipen
dc.subjectJatropha curcas and microsatellitesen
dc.titleCross species amplification ability of novel microsatellites isolated from Jatropha curcas and genetic relationship with sister taxa : Cross species amplification and genetic relationship of Jatropha using novel microsatellitesen
dc.typeArticleen
dc.contributor.departmentCenter for Desert Agricultureen
dc.identifier.journalMolecular Biology Reportsen
dc.contributor.institutionDiscipline of Wasteland Research, Central Salt and Marine Chemicals Research Institute (CSIR), Bhavnagar, Gujarat 364002, Indiaen
kaust.authorReddy, Muppala P.en
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