Vertical stratification of microbial communities in the Red Sea revealed by 16S rDNA pyrosequencing

Handle URI:
http://hdl.handle.net/10754/561624
Title:
Vertical stratification of microbial communities in the Red Sea revealed by 16S rDNA pyrosequencing
Authors:
Qian, Peiyuan; Wang, Yong; Lee, Onon; Lau, Chunkwan; Yang, Jiangke; Lafi, Feras Fawzi; Al-Suwailem, Abdulaziz M.; Wong, Tim
Abstract:
The ecosystems of the Red Sea are among the least-explored microbial habitats in the marine environment. In this study, we investigated the microbial communities in the water column overlying the Atlantis II Deep and Discovery Deep in the Red Sea. Taxonomic classification of pyrosequencing reads of the 16S rRNA gene amplicons showed vertical stratification of microbial diversity from the surface water to 1500 m below the surface. Significant differences in both bacterial and archaeal diversity were observed in the upper (2 and 50 m) and deeper layers (200 and 1500 m). There were no obvious differences in community structure at the same depth for the two sampling stations. The bacterial community in the upper layer was dominated by Cyanobacteria whereas the deeper layer harbored a large proportion of Proteobacteria. Among Archaea, Euryarchaeota, especially Halobacteriales, were dominant in the upper layer but diminished drastically in the deeper layer where Desulfurococcales belonging to Crenarchaeota became the dominant group. The results of our study indicate that the microbial communities sampled in this study are different from those identified in water column in other parts of the world. The depth-wise compositional variation in the microbial communities is attributable to their adaptations to the various environments in the Red Sea. © 2011 International Society for Microbial Ecology All rights reserved.
KAUST Department:
KAUST Global Collaborative Research Program; Center for Desert Agriculture; Coastal and Marine Resources Core Lab
Publisher:
Nature Publishing Group
Journal:
ISME Journal
Issue Date:
29-Jul-2010
DOI:
10.1038/ismej.2010.112
PubMed ID:
20668490
PubMed Central ID:
PMC3105721
Type:
Article
ISSN:
17517362
Sponsors:
We thank ML Sogin for his constructive comments on this article, the crew of the R/V Oceanus for providing technical assistance during the expedition and A Bower and S Swift of the Woods Hole Oceanographic Institution for providing environmental data and useful information of the study sites. We also thank R-L Liu of HKUST for the enumeration of microbial cells densities in water samples. This study was supported by an award (SA-C0040/UK-C0016) granted to PY Qian from the King Abdullah University of Science and Technology.
Additional Links:
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3105721
Appears in Collections:
Articles; Coastal and Marine Resources Core Lab; Center for Desert Agriculture

Full metadata record

DC FieldValue Language
dc.contributor.authorQian, Peiyuanen
dc.contributor.authorWang, Yongen
dc.contributor.authorLee, Ononen
dc.contributor.authorLau, Chunkwanen
dc.contributor.authorYang, Jiangkeen
dc.contributor.authorLafi, Feras Fawzien
dc.contributor.authorAl-Suwailem, Abdulaziz M.en
dc.contributor.authorWong, Timen
dc.date.accessioned2015-08-03T09:00:35Zen
dc.date.available2015-08-03T09:00:35Zen
dc.date.issued2010-07-29en
dc.identifier.issn17517362en
dc.identifier.pmid20668490en
dc.identifier.doi10.1038/ismej.2010.112en
dc.identifier.urihttp://hdl.handle.net/10754/561624en
dc.description.abstractThe ecosystems of the Red Sea are among the least-explored microbial habitats in the marine environment. In this study, we investigated the microbial communities in the water column overlying the Atlantis II Deep and Discovery Deep in the Red Sea. Taxonomic classification of pyrosequencing reads of the 16S rRNA gene amplicons showed vertical stratification of microbial diversity from the surface water to 1500 m below the surface. Significant differences in both bacterial and archaeal diversity were observed in the upper (2 and 50 m) and deeper layers (200 and 1500 m). There were no obvious differences in community structure at the same depth for the two sampling stations. The bacterial community in the upper layer was dominated by Cyanobacteria whereas the deeper layer harbored a large proportion of Proteobacteria. Among Archaea, Euryarchaeota, especially Halobacteriales, were dominant in the upper layer but diminished drastically in the deeper layer where Desulfurococcales belonging to Crenarchaeota became the dominant group. The results of our study indicate that the microbial communities sampled in this study are different from those identified in water column in other parts of the world. The depth-wise compositional variation in the microbial communities is attributable to their adaptations to the various environments in the Red Sea. © 2011 International Society for Microbial Ecology All rights reserved.en
dc.description.sponsorshipWe thank ML Sogin for his constructive comments on this article, the crew of the R/V Oceanus for providing technical assistance during the expedition and A Bower and S Swift of the Woods Hole Oceanographic Institution for providing environmental data and useful information of the study sites. We also thank R-L Liu of HKUST for the enumeration of microbial cells densities in water samples. This study was supported by an award (SA-C0040/UK-C0016) granted to PY Qian from the King Abdullah University of Science and Technology.en
dc.publisherNature Publishing Groupen
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3105721en
dc.subjectampliconsen
dc.subjectarchaeaen
dc.subjectbacteriaen
dc.subjectpyrosequencingen
dc.subjectRed Seaen
dc.subjectwater columnen
dc.titleVertical stratification of microbial communities in the Red Sea revealed by 16S rDNA pyrosequencingen
dc.typeArticleen
dc.contributor.departmentKAUST Global Collaborative Research Programen
dc.contributor.departmentCenter for Desert Agricultureen
dc.contributor.departmentCoastal and Marine Resources Core Laben
dc.identifier.journalISME Journalen
dc.identifier.pmcidPMC3105721en
kaust.authorWang, Yongen
kaust.authorLafi, Feras Fawzien
kaust.authorAl-Suwailem, Abdulaziz M.en

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