Towards the development of a direct electrochemical biodetector of avidin based on the poly(chloro amino β-styryl terthiophene)-coated glassy carbon electrode

Handle URI:
http://hdl.handle.net/10754/561615
Title:
Towards the development of a direct electrochemical biodetector of avidin based on the poly(chloro amino β-styryl terthiophene)-coated glassy carbon electrode
Authors:
Mehenni, Hakim; Dao, Lê Huynh Anh
Abstract:
In this study, a simple and direct biodetector was proposed, which was based on biotin immobilized onto a conducting polymer-coated electrode, for the detection of avidin, a highly stable glycoprotein found in egg-whites. Biotin was immobilized onto the electrode by covalent coupling to the primary amine group on the poly 3′-(3-chloro-4-amino-β-styryl)-(2,2′: 5′,2″-terthiophene) (PCAST), and the biotinavidin interaction was monitored by cyclic voltammetry. Incubation of the PCAST/biotin-modified-coated electrode with avidin in a phosphate buffered saline solution caused a significant change to its cyclic voltammogram, which was explained by the binding of avidin by biotin, and resulted in restricted ion transfer to and from the conducting polymer. This change was then utilized to detect avidin at 4 × 10 -6molL -1. © 2012 CSIRO.
KAUST Department:
Functional Nanomaterials Lab (FuNL); Materials Science and Engineering Program; KAUST Catalysis Center (KCC)
Publisher:
CSIRO Publishing
Journal:
Australian Journal of Chemistry
Issue Date:
30-Mar-2012
DOI:
10.1071/CH11397
Type:
Article
ISSN:
00049425
Sponsors:
This work was supported by the National Institute of Scientific Research (INRS) and the Natural Sciences and Engineering Research Council of Canada (NSERC).
Appears in Collections:
Articles; Materials Science and Engineering Program; KAUST Catalysis Center (KCC)

Full metadata record

DC FieldValue Language
dc.contributor.authorMehenni, Hakimen
dc.contributor.authorDao, Lê Huynh Anhen
dc.date.accessioned2015-08-03T09:00:09Zen
dc.date.available2015-08-03T09:00:09Zen
dc.date.issued2012-03-30en
dc.identifier.issn00049425en
dc.identifier.doi10.1071/CH11397en
dc.identifier.urihttp://hdl.handle.net/10754/561615en
dc.description.abstractIn this study, a simple and direct biodetector was proposed, which was based on biotin immobilized onto a conducting polymer-coated electrode, for the detection of avidin, a highly stable glycoprotein found in egg-whites. Biotin was immobilized onto the electrode by covalent coupling to the primary amine group on the poly 3′-(3-chloro-4-amino-β-styryl)-(2,2′: 5′,2″-terthiophene) (PCAST), and the biotinavidin interaction was monitored by cyclic voltammetry. Incubation of the PCAST/biotin-modified-coated electrode with avidin in a phosphate buffered saline solution caused a significant change to its cyclic voltammogram, which was explained by the binding of avidin by biotin, and resulted in restricted ion transfer to and from the conducting polymer. This change was then utilized to detect avidin at 4 × 10 -6molL -1. © 2012 CSIRO.en
dc.description.sponsorshipThis work was supported by the National Institute of Scientific Research (INRS) and the Natural Sciences and Engineering Research Council of Canada (NSERC).en
dc.publisherCSIRO Publishingen
dc.titleTowards the development of a direct electrochemical biodetector of avidin based on the poly(chloro amino β-styryl terthiophene)-coated glassy carbon electrodeen
dc.typeArticleen
dc.contributor.departmentFunctional Nanomaterials Lab (FuNL)en
dc.contributor.departmentMaterials Science and Engineering Programen
dc.contributor.departmentKAUST Catalysis Center (KCC)en
dc.identifier.journalAustralian Journal of Chemistryen
dc.contributor.institutionLaboratoires de Recherche sur les Matériaux Organiques Avancés, INRS-Energie et Matériaux, 1650 Bd Lionel Boulet, Varennes, QC J3X 1S2, Canadaen
kaust.authorMehenni, Hakimen
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