Differential Cotton leaf crumple virus-VIGS-mediated gene silencing and viral genome localization in different Gossypium hirsutum genetic backgrounds

Handle URI:
http://hdl.handle.net/10754/561574
Title:
Differential Cotton leaf crumple virus-VIGS-mediated gene silencing and viral genome localization in different Gossypium hirsutum genetic backgrounds
Authors:
Idris, Ali; Tuttle, John Richard; Robertson, Dominique Niki; Haigler, Candace H.; Brown, Judith K.
Abstract:
A Cotton leaf crumple virus (CLCrV)-based gene silencing vector containing a fragment of the Gossypium hirsutum Magnesium chelatase subunit I was used to establish endogenous gene silencing in cotton of varied genetic backgrounds. Biolistic inoculation resulted in systemic and persistent photo-bleaching of the leaves and bolls of the seven cultivars tested, however, the intensity of silencing was variable. CLCrV-VIGS-mediated expression of green fluorescent protein was used to monitor the in planta distribution of the vector, indicating successful phloem invasion in all cultivars tested. Acala SJ-1, one of the cotton cultivars, was identified as a particularly optimal candidate for CLCrV-VIGS-based cotton reverse-genetics. © 2010 Elsevier Ltd.
KAUST Department:
Center for Desert Agriculture
Publisher:
Elsevier
Journal:
Physiological and Molecular Plant Pathology
Issue Date:
1-Dec-2010
DOI:
10.1016/j.pmpp.2010.07.002
Type:
Article
ISSN:
08855765
Appears in Collections:
Articles; Center for Desert Agriculture

Full metadata record

DC FieldValue Language
dc.contributor.authorIdris, Alien
dc.contributor.authorTuttle, John Richarden
dc.contributor.authorRobertson, Dominique Nikien
dc.contributor.authorHaigler, Candace H.en
dc.contributor.authorBrown, Judith K.en
dc.date.accessioned2015-08-02T09:14:32Zen
dc.date.available2015-08-02T09:14:32Zen
dc.date.issued2010-12-01en
dc.identifier.issn08855765en
dc.identifier.doi10.1016/j.pmpp.2010.07.002en
dc.identifier.urihttp://hdl.handle.net/10754/561574en
dc.description.abstractA Cotton leaf crumple virus (CLCrV)-based gene silencing vector containing a fragment of the Gossypium hirsutum Magnesium chelatase subunit I was used to establish endogenous gene silencing in cotton of varied genetic backgrounds. Biolistic inoculation resulted in systemic and persistent photo-bleaching of the leaves and bolls of the seven cultivars tested, however, the intensity of silencing was variable. CLCrV-VIGS-mediated expression of green fluorescent protein was used to monitor the in planta distribution of the vector, indicating successful phloem invasion in all cultivars tested. Acala SJ-1, one of the cotton cultivars, was identified as a particularly optimal candidate for CLCrV-VIGS-based cotton reverse-genetics. © 2010 Elsevier Ltd.en
dc.publisherElsevieren
dc.subjectBegomovirusen
dc.subjectCotton breedingen
dc.subjectCotton geneticsen
dc.subjectGeminivirusen
dc.subjectGenetic improvementen
dc.subjectMagnesium chelatase markeren
dc.subjectViral episomeen
dc.titleDifferential Cotton leaf crumple virus-VIGS-mediated gene silencing and viral genome localization in different Gossypium hirsutum genetic backgroundsen
dc.typeArticleen
dc.contributor.departmentCenter for Desert Agricultureen
dc.identifier.journalPhysiological and Molecular Plant Pathologyen
dc.contributor.institutionSchool of Plant Sciences, University of Arizona, Tucson, AZ 85721, United Statesen
dc.contributor.institutionDepartment of Plant Biology, North Carolina University, Raleigh, NC 27695, United Statesen
dc.contributor.institutionDepartment of Crop Science, North Carolina University, Raleigh, NC 27695, United Statesen
kaust.authorIdris, Alien
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