Effect of nickel on regeneration in Jatropha curcas L. and assessment of genotoxicity using RAPD markers

Handle URI:
http://hdl.handle.net/10754/561568
Title:
Effect of nickel on regeneration in Jatropha curcas L. and assessment of genotoxicity using RAPD markers
Authors:
Sarkar, Tanmoy; Vijay Anand, K. G.; Reddy, Muppala P.
Abstract:
The aim of the present study was to determine the effect of nickel on shoot regeneration in tissue culture as well as to identify polymorphisms induced in leaf explants exposed to nickel through random amplified polymorphic DNA (RAPD). In vitro leaf explants of Jatropha curcas were grown in nickel amended Murashige and Skoog (MS) medium at four different concentrations (0, 0.01, 0.1, 1 mM) for 3 weeks. Percent regeneration, number of shoots produced and genotoxic effects were evaluated by RAPD using leaf explants obtained from the first three treatments following 5 weeks of their subsequent subculture in metal free MS medium. Percent regeneration decreased with increase in addition of nickel to the medium up to 14 days from 42.31% in control to zero in 1.0 mM. The number of shoot buds scored after 5 weeks was higher in control as compared to all other treatments except in one of the metal free subculture medium wherein the shoot number was higher in 0.01 mM treatment (mean = 7.80) than control (mean = 7.60). RAPD analysis produced only 5 polymorphic bands (3.225%) out of a total of 155 bands from 18 selected primers. Only three primers OPK-19, OPP-2, OPN-08 produced polymorphic bands. The dendrogram showed three groups A, B, and C. Group A samples showed 100% genetic similarity within them. Samples between groups B and C were more genetically distant from each other as compared to samples between groups A and B as well as groups A and C. Cluster analysis based on RAPD data correlated with treatments. © 2010 Springer Science+Business Media, LLC.
KAUST Department:
Center for Desert Agriculture
Publisher:
Springer Verlag
Journal:
BioMetals
Issue Date:
8-Jul-2010
DOI:
10.1007/s10534-010-9364-7
PubMed ID:
20614165
Type:
Article
ISSN:
09660844
Appears in Collections:
Articles; Center for Desert Agriculture

Full metadata record

DC FieldValue Language
dc.contributor.authorSarkar, Tanmoyen
dc.contributor.authorVijay Anand, K. G.en
dc.contributor.authorReddy, Muppala P.en
dc.date.accessioned2015-08-02T09:14:24Zen
dc.date.available2015-08-02T09:14:24Zen
dc.date.issued2010-07-08en
dc.identifier.issn09660844en
dc.identifier.pmid20614165en
dc.identifier.doi10.1007/s10534-010-9364-7en
dc.identifier.urihttp://hdl.handle.net/10754/561568en
dc.description.abstractThe aim of the present study was to determine the effect of nickel on shoot regeneration in tissue culture as well as to identify polymorphisms induced in leaf explants exposed to nickel through random amplified polymorphic DNA (RAPD). In vitro leaf explants of Jatropha curcas were grown in nickel amended Murashige and Skoog (MS) medium at four different concentrations (0, 0.01, 0.1, 1 mM) for 3 weeks. Percent regeneration, number of shoots produced and genotoxic effects were evaluated by RAPD using leaf explants obtained from the first three treatments following 5 weeks of their subsequent subculture in metal free MS medium. Percent regeneration decreased with increase in addition of nickel to the medium up to 14 days from 42.31% in control to zero in 1.0 mM. The number of shoot buds scored after 5 weeks was higher in control as compared to all other treatments except in one of the metal free subculture medium wherein the shoot number was higher in 0.01 mM treatment (mean = 7.80) than control (mean = 7.60). RAPD analysis produced only 5 polymorphic bands (3.225%) out of a total of 155 bands from 18 selected primers. Only three primers OPK-19, OPP-2, OPN-08 produced polymorphic bands. The dendrogram showed three groups A, B, and C. Group A samples showed 100% genetic similarity within them. Samples between groups B and C were more genetically distant from each other as compared to samples between groups A and B as well as groups A and C. Cluster analysis based on RAPD data correlated with treatments. © 2010 Springer Science+Business Media, LLC.en
dc.publisherSpringer Verlagen
dc.subjectGenotoxicityen
dc.subjectJatropha curcasen
dc.subjectMolecular markersen
dc.subjectNickelen
dc.subjectRegenerationen
dc.titleEffect of nickel on regeneration in Jatropha curcas L. and assessment of genotoxicity using RAPD markersen
dc.typeArticleen
dc.contributor.departmentCenter for Desert Agricultureen
dc.identifier.journalBioMetalsen
dc.contributor.institutionDiscipline of Wasteland Research, Central Salt and Marine Chemicals Research Institute (CSIR), Bhavnagar, Gujarat 364002, Indiaen
kaust.authorReddy, Muppala P.en

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