Supra-optimal expression of the cold-regulated OsMyb4 transcription factor in transgenic rice changes the complexity of transcriptional network with major effects on stress tolerance and panicle development

Handle URI:
http://hdl.handle.net/10754/561565
Title:
Supra-optimal expression of the cold-regulated OsMyb4 transcription factor in transgenic rice changes the complexity of transcriptional network with major effects on stress tolerance and panicle development
Authors:
Park, Myoungryoul; Yun, Kilyoung; Mohanty, Bijayalaxmi; Herath, Venura; Xu, Fuyu; Wijaya, Edward; Bajic, Vladimir B. ( 0000-0001-5435-4750 ) ; Yun, Songjoong; De Los Reyes, Benildo G.
Abstract:
The R2R3-type OsMyb4 transcription factor of rice has been shown to play a role in the regulation of osmotic adjustment in heterologous overexpression studies. However, the exact composition and organization of its underlying transcriptional network has not been established to be a robust tool for stress tolerance enhancement by regulon engineering. OsMyb4 network was dissected based on commonalities between the global chilling stress transcriptome and the transcriptome configured by OsMyb4 overexpression. OsMyb4 controls a hierarchical network comprised of several regulatory sub-clusters associated with cellular defense and rescue, metabolism and development. It regulates target genes either directly or indirectly through intermediary MYB, ERF, bZIP, NAC, ARF and CCAAT-HAP transcription factors. Regulatory sub-clusters have different combinations of MYB-like, GCC-box-like, ERD1-box-like, ABRE-like, G-box-like, as1/ocs/TGA-like, AuxRE-like, gibberellic acid response element (GARE)-like and JAre-like cis-elements. Cold-dependent network activity enhanced cellular antioxidant capacity through radical scavenging mechanisms and increased activities of phenylpropanoid and isoprenoid metabolic processes involving various abscisic acid (ABA), jasmonic acid (JA), salicylic acid (SA), ethylene and reactive oxygen species (ROS) responsive genes. OsMyb4 network is independent of drought response element binding protein/C-repeat binding factor (DREB/CBF) and its sub-regulons operate with possible co-regulators including nuclear factor-Y. Because of its upstream position in the network hierarchy, OsMyb4 functions quantitatively and pleiotrophically. Supra-optimal expression causes misexpression of alternative targets with costly trade-offs to panicle development. © 2010 Blackwell Publishing Ltd.
KAUST Department:
Computational Bioscience Research Center (CBRC); Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division; Applied Mathematics and Computational Science Program
Publisher:
Wiley-Blackwell
Journal:
Plant, Cell & Environment
Issue Date:
28-Sep-2010
DOI:
10.1111/j.1365-3040.2010.02221.x
PubMed ID:
20807373
Type:
Article
ISSN:
01407791
Sponsors:
This study was funded by the United States Department of Agriculture (USDA) National Research Initiative (2006-35604-1669) and Maine Agricultural and Forestry Experiment Station (Publication 3104). M.-R.P. was a recipient of the Korea Research Foundation Fellowship (KRF-2006-352-F00002). S.-J.Y. and M.-R.P. were supported by BioGreen 21 Program-RDA (20080401034024), Republic of Korea. We also thank Dr Yulin Jia and the two anonymous reviewers for their insightful suggestions.
Appears in Collections:
Articles; Applied Mathematics and Computational Science Program; Computational Bioscience Research Center (CBRC); Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorPark, Myoungryoulen
dc.contributor.authorYun, Kilyoungen
dc.contributor.authorMohanty, Bijayalaxmien
dc.contributor.authorHerath, Venuraen
dc.contributor.authorXu, Fuyuen
dc.contributor.authorWijaya, Edwarden
dc.contributor.authorBajic, Vladimir B.en
dc.contributor.authorYun, Songjoongen
dc.contributor.authorDe Los Reyes, Benildo G.en
dc.date.accessioned2015-08-02T09:14:20Zen
dc.date.available2015-08-02T09:14:20Zen
dc.date.issued2010-09-28en
dc.identifier.issn01407791en
dc.identifier.pmid20807373en
dc.identifier.doi10.1111/j.1365-3040.2010.02221.xen
dc.identifier.urihttp://hdl.handle.net/10754/561565en
dc.description.abstractThe R2R3-type OsMyb4 transcription factor of rice has been shown to play a role in the regulation of osmotic adjustment in heterologous overexpression studies. However, the exact composition and organization of its underlying transcriptional network has not been established to be a robust tool for stress tolerance enhancement by regulon engineering. OsMyb4 network was dissected based on commonalities between the global chilling stress transcriptome and the transcriptome configured by OsMyb4 overexpression. OsMyb4 controls a hierarchical network comprised of several regulatory sub-clusters associated with cellular defense and rescue, metabolism and development. It regulates target genes either directly or indirectly through intermediary MYB, ERF, bZIP, NAC, ARF and CCAAT-HAP transcription factors. Regulatory sub-clusters have different combinations of MYB-like, GCC-box-like, ERD1-box-like, ABRE-like, G-box-like, as1/ocs/TGA-like, AuxRE-like, gibberellic acid response element (GARE)-like and JAre-like cis-elements. Cold-dependent network activity enhanced cellular antioxidant capacity through radical scavenging mechanisms and increased activities of phenylpropanoid and isoprenoid metabolic processes involving various abscisic acid (ABA), jasmonic acid (JA), salicylic acid (SA), ethylene and reactive oxygen species (ROS) responsive genes. OsMyb4 network is independent of drought response element binding protein/C-repeat binding factor (DREB/CBF) and its sub-regulons operate with possible co-regulators including nuclear factor-Y. Because of its upstream position in the network hierarchy, OsMyb4 functions quantitatively and pleiotrophically. Supra-optimal expression causes misexpression of alternative targets with costly trade-offs to panicle development. © 2010 Blackwell Publishing Ltd.en
dc.description.sponsorshipThis study was funded by the United States Department of Agriculture (USDA) National Research Initiative (2006-35604-1669) and Maine Agricultural and Forestry Experiment Station (Publication 3104). M.-R.P. was a recipient of the Korea Research Foundation Fellowship (KRF-2006-352-F00002). S.-J.Y. and M.-R.P. were supported by BioGreen 21 Program-RDA (20080401034024), Republic of Korea. We also thank Dr Yulin Jia and the two anonymous reviewers for their insightful suggestions.en
dc.publisherWiley-Blackwellen
dc.subjectDREB independenten
dc.subjectOxidative injuryen
dc.subjectR2R3-MYBen
dc.subjectRadical scavengingen
dc.subjectRegulon engineeringen
dc.subjectTranscriptional networken
dc.titleSupra-optimal expression of the cold-regulated OsMyb4 transcription factor in transgenic rice changes the complexity of transcriptional network with major effects on stress tolerance and panicle developmenten
dc.typeArticleen
dc.contributor.departmentComputational Bioscience Research Center (CBRC)en
dc.contributor.departmentComputer, Electrical and Mathematical Sciences and Engineering (CEMSE) Divisionen
dc.contributor.departmentApplied Mathematics and Computational Science Programen
dc.identifier.journalPlant, Cell & Environmenten
dc.contributor.institutionSchool of Biology and Ecology, University of Maine, Orono, ME 04469, United Statesen
dc.contributor.institutionDepartment of Crop Science and Institute of Agricultural Science and Technology, Chonbuk National University, Chonju 561-756, South Koreaen
dc.contributor.institutionDepartment of Chemical and Biomolecular Engineering, National University of Singapore, Singapore 117576, Singaporeen
dc.contributor.institutionComputational Biology Research Center, AIST Tokyo Waterfront, 2-41-6 Aomi, Koto-ku, Tokyo 135-0064, Japanen
kaust.authorBajic, Vladimir B.en

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