Isolation of novel microsatellites using FIASCO by dual probe enrichment from Jatropha curcas L. and study on genetic equilibrium and diversity of Indian population revealed by isolated microsatellites

Handle URI:
http://hdl.handle.net/10754/561563
Title:
Isolation of novel microsatellites using FIASCO by dual probe enrichment from Jatropha curcas L. and study on genetic equilibrium and diversity of Indian population revealed by isolated microsatellites
Authors:
Pamidimarri, D. V N N Sudheer; Rahman, Hifzur; Mastan, Shaik G.; Reddy, Muppala P.
Abstract:
Jatropha curcas L. belongs to family Euphorbiaceae, native to South America attained significant importance for its seed oil which can be converted to biodiesel, a renewable energy source alternative to conventional petrodiesel. Very few attempts were made to isolate novel microsatellite markers and assessment of the extent of genetic equilibrium and diversity that exists in J. curcas. Therefore, the present investigation was undertaken to isolate the novel microsatellites and access genetic equilibrium, diversity that exists among 44 diverse germplasm collected from distinct geographical areas in India using isolated microsatellites. The overall efficiency of the enrichment of microsatellite by dual probe in the present study found to be 54% and among the sequences obtained the percentage of sequences having suitable flanking regions for the primer designing was found to be 89.58%. The mean co-efficient of genetic similarity (CGS) was found to be 0.97. The overall diversity obtained by microsatellites was found to be low in comparison with the diversity reported by multilocus markers systems observed in earlier studies; however, the good allele polymorphism was observed. The overall dendrogram of microsatellite analysis resulted in random clustering of germplasm and not in accordance to geographical area of collection. The present study, diversity analysis using microsatellite markers concludes the low genetic diversity and genetic disequlibrium of J. curcas in India and will provide pavement for further intra-population studies on narrow geographical areas to understand the population genetic structure, phylogeography and molecular ecological studies. The germplasm characterized, and the microsatellite markers isolated and characterized in the present study can be employed efficiently in breeding programs for genetic improvement of the species through marker assisted selection and QTL analysis, for further genetic resource management and help in making the J. curcas as potential crop with superior agronomical traits. © 2010 Springer Science+Business Media B.V.
KAUST Department:
Center for Desert Agriculture
Publisher:
Springer Nature
Journal:
Molecular Biology Reports
Issue Date:
11-Mar-2010
DOI:
10.1007/s11033-010-0033-2
PubMed ID:
20221701
Type:
Article
ISSN:
03014851
Sponsors:
The authors wish to thank Council for Scientific and Industrial Research (CSIR), New Delhi, India for financial support and for the Research Associate fellowship.
Appears in Collections:
Articles; Center for Desert Agriculture

Full metadata record

DC FieldValue Language
dc.contributor.authorPamidimarri, D. V N N Sudheeren
dc.contributor.authorRahman, Hifzuren
dc.contributor.authorMastan, Shaik G.en
dc.contributor.authorReddy, Muppala P.en
dc.date.accessioned2015-08-02T09:14:17Zen
dc.date.available2015-08-02T09:14:17Zen
dc.date.issued2010-03-11en
dc.identifier.issn03014851en
dc.identifier.pmid20221701en
dc.identifier.doi10.1007/s11033-010-0033-2en
dc.identifier.urihttp://hdl.handle.net/10754/561563en
dc.description.abstractJatropha curcas L. belongs to family Euphorbiaceae, native to South America attained significant importance for its seed oil which can be converted to biodiesel, a renewable energy source alternative to conventional petrodiesel. Very few attempts were made to isolate novel microsatellite markers and assessment of the extent of genetic equilibrium and diversity that exists in J. curcas. Therefore, the present investigation was undertaken to isolate the novel microsatellites and access genetic equilibrium, diversity that exists among 44 diverse germplasm collected from distinct geographical areas in India using isolated microsatellites. The overall efficiency of the enrichment of microsatellite by dual probe in the present study found to be 54% and among the sequences obtained the percentage of sequences having suitable flanking regions for the primer designing was found to be 89.58%. The mean co-efficient of genetic similarity (CGS) was found to be 0.97. The overall diversity obtained by microsatellites was found to be low in comparison with the diversity reported by multilocus markers systems observed in earlier studies; however, the good allele polymorphism was observed. The overall dendrogram of microsatellite analysis resulted in random clustering of germplasm and not in accordance to geographical area of collection. The present study, diversity analysis using microsatellite markers concludes the low genetic diversity and genetic disequlibrium of J. curcas in India and will provide pavement for further intra-population studies on narrow geographical areas to understand the population genetic structure, phylogeography and molecular ecological studies. The germplasm characterized, and the microsatellite markers isolated and characterized in the present study can be employed efficiently in breeding programs for genetic improvement of the species through marker assisted selection and QTL analysis, for further genetic resource management and help in making the J. curcas as potential crop with superior agronomical traits. © 2010 Springer Science+Business Media B.V.en
dc.description.sponsorshipThe authors wish to thank Council for Scientific and Industrial Research (CSIR), New Delhi, India for financial support and for the Research Associate fellowship.en
dc.publisherSpringer Natureen
dc.subjectBiodieselen
dc.subjectGenetic diversityen
dc.subjectJ. curcasen
dc.subjectMicrosatellitesen
dc.titleIsolation of novel microsatellites using FIASCO by dual probe enrichment from Jatropha curcas L. and study on genetic equilibrium and diversity of Indian population revealed by isolated microsatellitesen
dc.typeArticleen
dc.contributor.departmentCenter for Desert Agricultureen
dc.identifier.journalMolecular Biology Reportsen
dc.contributor.institutionDiscipline of Wasteland Research, Central Salt and Marine Chemicals Research Institute (CSIR), Bhavnagar, Gujarat 364002, Indiaen
dc.contributor.institutionShree M. and N. Virani Science College, Saurashtra University, Rajkot, Gujarat 360005, Indiaen
kaust.authorReddy, Muppala P.en
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