The DOF transcription factor Dof5.1 influences leaf axial patterning by promoting Revoluta transcription in Arabidopsis

Handle URI:
http://hdl.handle.net/10754/561562
Title:
The DOF transcription factor Dof5.1 influences leaf axial patterning by promoting Revoluta transcription in Arabidopsis
Authors:
Kim, Hyungsae; Kim, Sungjin; Abbasi, Nazia; Bressan, Ray Anthony; Yun, Daejin; Yoo, Sangdong; Kwon, SukYun; Choi, Sangbong
Abstract:
Dof proteins are transcription factors that have a conserved single zinc finger DNA-binding domain. In this study, we isolated an activation tagging mutant Dof5.1-D exhibiting an upward-curling leaf phenotype due to enhanced expression of the REV gene that is required for establishing adaxialabaxial polarity. Dof5.1-D plants also had reduced transcript levels for IAA6 and IAA19 genes, indicating an altered auxin biosynthesis in Dof5.1-D. An electrophoretic mobility shift assay using the Dof5.1 DNA-binding motif and the REV promoter region indicated that the DNA-binding domain of Dof5.1 binds to a TAAAGT motif located in the 5′-distal promoter region of the REV promoter. Further, transient and chromatin immunoprecipitation assays verified binding activity of the Dof5.1 DNA-binding motif with the REV promoter. Consistent with binding assays, constitutive over-expression of the Dof5.1 DNA-binding domain in wild-type plants caused a downward-curling phenotype, whereas crossing Dof5.1-D to a rev mutant reverted the upward-curling phenotype of the Dof5.1-D mutant leaf to the wild-type. These results suggest that the Dof5.1 protein directly binds to the REV promoter and thereby regulates adaxialabaxial polarity. © 2010 Blackwell Publishing Ltd.
KAUST Department:
Center for Desert Agriculture; Biological and Environmental Sciences and Engineering (BESE) Division
Publisher:
Wiley
Journal:
Plant Journal
Issue Date:
5-Oct-2010
DOI:
10.1111/j.1365-313X.2010.04346.x
PubMed ID:
20807212
Type:
Article
ISSN:
09607412
Sponsors:
We thank Gyung-Tae Kim for the rev-6 seeds and Yul-Ho Kim for the SEM pictures. S.-B. Choi and S.-Y. Kwon were supported by Bio-Green21 (grant 20080401034045 from the Rural Development Administration), and S.-B. Choi (CG2151) and S.-Y. Kwon (CG2120-1) were supported by the Crop Functional Genomics Center funded by the Ministry of Education, Science and Technology, Korea. S.-D. Yoo is supported by NRF (2010-0016898). R.A. Bressan and D.-J. Yun are supported by the WCU program (grant R32-10148).
Appears in Collections:
Articles; Center for Desert Agriculture; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorKim, Hyungsaeen
dc.contributor.authorKim, Sungjinen
dc.contributor.authorAbbasi, Naziaen
dc.contributor.authorBressan, Ray Anthonyen
dc.contributor.authorYun, Daejinen
dc.contributor.authorYoo, Sangdongen
dc.contributor.authorKwon, SukYunen
dc.contributor.authorChoi, Sangbongen
dc.date.accessioned2015-08-02T09:14:16Zen
dc.date.available2015-08-02T09:14:16Zen
dc.date.issued2010-10-05en
dc.identifier.issn09607412en
dc.identifier.pmid20807212en
dc.identifier.doi10.1111/j.1365-313X.2010.04346.xen
dc.identifier.urihttp://hdl.handle.net/10754/561562en
dc.description.abstractDof proteins are transcription factors that have a conserved single zinc finger DNA-binding domain. In this study, we isolated an activation tagging mutant Dof5.1-D exhibiting an upward-curling leaf phenotype due to enhanced expression of the REV gene that is required for establishing adaxialabaxial polarity. Dof5.1-D plants also had reduced transcript levels for IAA6 and IAA19 genes, indicating an altered auxin biosynthesis in Dof5.1-D. An electrophoretic mobility shift assay using the Dof5.1 DNA-binding motif and the REV promoter region indicated that the DNA-binding domain of Dof5.1 binds to a TAAAGT motif located in the 5′-distal promoter region of the REV promoter. Further, transient and chromatin immunoprecipitation assays verified binding activity of the Dof5.1 DNA-binding motif with the REV promoter. Consistent with binding assays, constitutive over-expression of the Dof5.1 DNA-binding domain in wild-type plants caused a downward-curling phenotype, whereas crossing Dof5.1-D to a rev mutant reverted the upward-curling phenotype of the Dof5.1-D mutant leaf to the wild-type. These results suggest that the Dof5.1 protein directly binds to the REV promoter and thereby regulates adaxialabaxial polarity. © 2010 Blackwell Publishing Ltd.en
dc.description.sponsorshipWe thank Gyung-Tae Kim for the rev-6 seeds and Yul-Ho Kim for the SEM pictures. S.-B. Choi and S.-Y. Kwon were supported by Bio-Green21 (grant 20080401034045 from the Rural Development Administration), and S.-B. Choi (CG2151) and S.-Y. Kwon (CG2120-1) were supported by the Crop Functional Genomics Center funded by the Ministry of Education, Science and Technology, Korea. S.-D. Yoo is supported by NRF (2010-0016898). R.A. Bressan and D.-J. Yun are supported by the WCU program (grant R32-10148).en
dc.publisherWileyen
dc.subjectActivation tagging mutanten
dc.subjectDof transcription factoren
dc.subjectDof5.1en
dc.subjectLeaf polarityen
dc.subjectRevolutaen
dc.titleThe DOF transcription factor Dof5.1 influences leaf axial patterning by promoting Revoluta transcription in Arabidopsisen
dc.typeArticleen
dc.contributor.departmentCenter for Desert Agricultureen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalPlant Journalen
dc.contributor.institutionDivision of Bioscience and Bioinformatics, Myongji University, Yongin, Kyunggi-do 449-728, South Koreaen
dc.contributor.institutionSchool of Biotechnology and Environmental Engineering, Myongji University, Yongin, Kyunggi-do 449-728, South Koreaen
dc.contributor.institutionDepartment of Horticulture and Landscape Architecture, Center for Plant Environmental Stress Physiology, Purdue University, West Lafayette, IN 47907-2010, United Statesen
dc.contributor.institutionDivision of Applied Life Science, Graduate School of Gyeongsang, National University, Jinju 660701, South Koreaen
dc.contributor.institutionDepartment of Biological Science, College of Natural Science, SungKyunKwan University, Suwon, Kyunggi-do 440-746, South Koreaen
dc.contributor.institutionKorea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-333, South Koreaen
kaust.authorBressan, Ray Anthonyen

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