Shoot regeneration from cotyledonary leaf explants of jatropha curcas: A biodiesel plant

Handle URI:
http://hdl.handle.net/10754/561555
Title:
Shoot regeneration from cotyledonary leaf explants of jatropha curcas: A biodiesel plant
Authors:
Kumar, Nitish Chandramohana; Vijay Anand, K. G.; Reddy, Muppala P.
Abstract:
A simple, high frequency, and reproducible method for plant regeneration through direct organogenesis from cotyledonary leaf explants of Jatropha curcas was developed using Murashige and Skoog (MS) medium supplemented with different concentrations of thidiazuron (TDZ) or 6-benzyl aminopurine (BAP). Medium containing TDZ has greater influence on regeneration as compared to BAP. The induced shoot buds were transferred to MS medium containing 10 lM kinetin (Kn), 4.5 lM BAP, and 5.5 lM a-naphthaleneacetic acid (NAA) for shoot proliferation. The proliferated shoots could be elongated on MS medium supplemented with different concentrations and combinations of BAP, indole-3-acetic acid (IAA), NAA, and indole-3-butyric acid (IBA). MS medium with 2.25 lM BAP and 8.5 lM IAA was found to be the best combination for shoot elongation. However, significant differences in plant regeneration and shoot elongation were observed among the genotypes studied. Rooting was achieved when the basal cut end of elongated shoots were dipped in half strength MS liquid medium containing dif- ferent concentrations and combinations of IBA, IAA, and NAA for 4 days, followed by transfer to growth regulators free half strength MS medium supplemented 0.25 mg l-1 activated charcoal. Elongated shoot treated with 15 lM IBA, 5.7 lM IAA, and 11 lM NAA resulted in highest percent rooting. The rooted plants could be established in soil with more than 90% survival rate. The method developed may be useful in improvement of J. curcas through genetic modification. © Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2010.
KAUST Department:
Center for Desert Agriculture
Publisher:
Springer Nature
Journal:
Acta Physiologiae Plantarum
Issue Date:
7-Mar-2010
DOI:
10.1007/s11738-010-0479-9
Type:
Article
ISSN:
01375881
Sponsors:
The authors gratefully acknowledge Council of Scientific and Industrial Research, New Delhi, India for financial assistance. Statistical analysis support from Mr. Keyur Shah and Mr. Amritpal singh, Assistant professor, Department of Agricultural Biotechnology, Anand Agricultural University, Anand, India is thankfully acknowledged.
Appears in Collections:
Articles; Center for Desert Agriculture

Full metadata record

DC FieldValue Language
dc.contributor.authorKumar, Nitish Chandramohanaen
dc.contributor.authorVijay Anand, K. G.en
dc.contributor.authorReddy, Muppala P.en
dc.date.accessioned2015-08-02T09:14:06Zen
dc.date.available2015-08-02T09:14:06Zen
dc.date.issued2010-03-07en
dc.identifier.issn01375881en
dc.identifier.doi10.1007/s11738-010-0479-9en
dc.identifier.urihttp://hdl.handle.net/10754/561555en
dc.description.abstractA simple, high frequency, and reproducible method for plant regeneration through direct organogenesis from cotyledonary leaf explants of Jatropha curcas was developed using Murashige and Skoog (MS) medium supplemented with different concentrations of thidiazuron (TDZ) or 6-benzyl aminopurine (BAP). Medium containing TDZ has greater influence on regeneration as compared to BAP. The induced shoot buds were transferred to MS medium containing 10 lM kinetin (Kn), 4.5 lM BAP, and 5.5 lM a-naphthaleneacetic acid (NAA) for shoot proliferation. The proliferated shoots could be elongated on MS medium supplemented with different concentrations and combinations of BAP, indole-3-acetic acid (IAA), NAA, and indole-3-butyric acid (IBA). MS medium with 2.25 lM BAP and 8.5 lM IAA was found to be the best combination for shoot elongation. However, significant differences in plant regeneration and shoot elongation were observed among the genotypes studied. Rooting was achieved when the basal cut end of elongated shoots were dipped in half strength MS liquid medium containing dif- ferent concentrations and combinations of IBA, IAA, and NAA for 4 days, followed by transfer to growth regulators free half strength MS medium supplemented 0.25 mg l-1 activated charcoal. Elongated shoot treated with 15 lM IBA, 5.7 lM IAA, and 11 lM NAA resulted in highest percent rooting. The rooted plants could be established in soil with more than 90% survival rate. The method developed may be useful in improvement of J. curcas through genetic modification. © Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2010.en
dc.description.sponsorshipThe authors gratefully acknowledge Council of Scientific and Industrial Research, New Delhi, India for financial assistance. Statistical analysis support from Mr. Keyur Shah and Mr. Amritpal singh, Assistant professor, Department of Agricultural Biotechnology, Anand Agricultural University, Anand, India is thankfully acknowledged.en
dc.publisherSpringer Natureen
dc.subjectCotyledonary leafen
dc.subjectGenotypeen
dc.subjectJatropha curcasen
dc.subjectRegenerationen
dc.titleShoot regeneration from cotyledonary leaf explants of jatropha curcas: A biodiesel planten
dc.typeArticleen
dc.contributor.departmentCenter for Desert Agricultureen
dc.identifier.journalActa Physiologiae Plantarumen
dc.contributor.institutionDepartment of Agricultural Biotechnology, Anand Agricultural University, Anand 388110, Gujarat, Indiaen
dc.contributor.institutionDiscipline of Wasteland Research, Central Salt and Marine Chemicals Research Institute, Council of Scientific and Industrial Research, Bhavnagar 364021, Gujarat, Indiaen
kaust.authorReddy, Muppala P.en
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