Transcriptional Response of Rhodococcus aetherivorans I24 to Polychlorinated Biphenyl-Contaminated Sediments

Handle URI:
http://hdl.handle.net/10754/561549
Title:
Transcriptional Response of Rhodococcus aetherivorans I24 to Polychlorinated Biphenyl-Contaminated Sediments
Authors:
Puglisi, Edoardo; Cahill, Matt J.; Lessard, Philip A.; Capri, Ettore; Sinskey, Anthony John; Archer, John A.C. ( 0000-0002-3302-3933 ) ; Boccazzi, Paolo
Abstract:
We used a microarray targeting 3,524 genes to assess the transcriptional response of the actinomycete Rhodococcus aetherivorans I24 in minimal medium supplemented with various substrates (e. g., PCBs) and in both PCB-contaminated and non-contaminated sediment slurries. Relative to the reference condition (minimal medium supplemented with glucose), 408 genes were upregulated in the various treatments. In medium and in sediment, PCBs elicited the upregulation of a common set of 100 genes, including gene-encoding chaperones (groEL), a superoxide dismutase (sodA), alkyl hydroperoxide reductase protein C (ahpC), and a catalase/peroxidase (katG). Analysis of the R. aetherivorans I24 genome sequence identified orthologs of many of the genes in the canonical biphenyl pathway, but very few of these genes were upregulated in response to PCBs or biphenyl. This study is one of the first to use microarrays to assess the transcriptional response of a soil bacterium to a pollutant under conditions that more closely resemble the natural environment. Our results indicate that the transcriptional response of R. aetherivorans I24 to PCBs, in both medium and sediment, is primarily directed towards reducing oxidative stress, rather than catabolism. © 2010 Springer Science+Business Media, LLC.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Computational Bioscience Research Center (CBRC)
Publisher:
Springer Nature
Journal:
Microbial Ecology
Issue Date:
6-Apr-2010
DOI:
10.1007/s00248-010-9650-5
PubMed ID:
20369357
Type:
Article
ISSN:
00953628
Sponsors:
This work was supported by IRG Marie Curie Grant "COMEHERE," contract No. 21634, and by the Cambridge-MIT Institute.
Appears in Collections:
Articles; Computational Bioscience Research Center (CBRC); Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorPuglisi, Edoardoen
dc.contributor.authorCahill, Matt J.en
dc.contributor.authorLessard, Philip A.en
dc.contributor.authorCapri, Ettoreen
dc.contributor.authorSinskey, Anthony Johnen
dc.contributor.authorArcher, John A.C.en
dc.contributor.authorBoccazzi, Paoloen
dc.date.accessioned2015-08-02T09:13:58Zen
dc.date.available2015-08-02T09:13:58Zen
dc.date.issued2010-04-06en
dc.identifier.issn00953628en
dc.identifier.pmid20369357en
dc.identifier.doi10.1007/s00248-010-9650-5en
dc.identifier.urihttp://hdl.handle.net/10754/561549en
dc.description.abstractWe used a microarray targeting 3,524 genes to assess the transcriptional response of the actinomycete Rhodococcus aetherivorans I24 in minimal medium supplemented with various substrates (e. g., PCBs) and in both PCB-contaminated and non-contaminated sediment slurries. Relative to the reference condition (minimal medium supplemented with glucose), 408 genes were upregulated in the various treatments. In medium and in sediment, PCBs elicited the upregulation of a common set of 100 genes, including gene-encoding chaperones (groEL), a superoxide dismutase (sodA), alkyl hydroperoxide reductase protein C (ahpC), and a catalase/peroxidase (katG). Analysis of the R. aetherivorans I24 genome sequence identified orthologs of many of the genes in the canonical biphenyl pathway, but very few of these genes were upregulated in response to PCBs or biphenyl. This study is one of the first to use microarrays to assess the transcriptional response of a soil bacterium to a pollutant under conditions that more closely resemble the natural environment. Our results indicate that the transcriptional response of R. aetherivorans I24 to PCBs, in both medium and sediment, is primarily directed towards reducing oxidative stress, rather than catabolism. © 2010 Springer Science+Business Media, LLC.en
dc.description.sponsorshipThis work was supported by IRG Marie Curie Grant "COMEHERE," contract No. 21634, and by the Cambridge-MIT Institute.en
dc.publisherSpringer Natureen
dc.titleTranscriptional Response of Rhodococcus aetherivorans I24 to Polychlorinated Biphenyl-Contaminated Sedimentsen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentComputational Bioscience Research Center (CBRC)en
dc.identifier.journalMicrobial Ecologyen
dc.contributor.institutionIstituto di Chimica Agraria ed Ambientale, Università Cattolica del Sacro Cuore, Piacenza, Italyen
dc.contributor.institutionDepartment of Biology, Massachusetts Institute of Technology, 77 Massachusetts Ave. 68-370A, Cambridge, MA 02139, United Statesen
dc.contributor.institutionDepartment of Genetics, University of Cambridge, Cambridge CB2 3EH, United Kingdomen
kaust.authorArcher, John A.C.en

Related articles on PubMed

All Items in KAUST are protected by copyright, with all rights reserved, unless otherwise indicated.