Solid-state NMR paramagnetic relaxation enhancement immersion depth studies in phospholipid bilayers

Handle URI:
http://hdl.handle.net/10754/561548
Title:
Solid-state NMR paramagnetic relaxation enhancement immersion depth studies in phospholipid bilayers
Authors:
Chu, Shidong; Maltsev, Sergey B.; Emwas, Abdul-Hamid M.; Lorigan, Gary A.
Abstract:
A new approach for determining the membrane immersion depth of a spin-labeled probe has been developed using paramagnetic relaxation enhancement (PRE) in solid-state NMR spectroscopy. A DOXYL spin label was placed at different sites of 1-palmitoyl-2-stearoyl-sn-glycero-3-phosphocholine (PSPC) phospholipid bilayers as paramagnetic moieties and the resulting enhancements of the longitudinal relaxation (T1) times of 31P nuclei on the surface of the bilayers were measured by a standard inversion recovery pulse sequence. The 31P NMR spin-lattice relaxation times decrease steadily as the DOXYL spin label moves closer to the surface as well as the concentration of the spin-labeled lipids increase. The enhanced relaxation vs. the position and concentration of spin-labels indicate that PRE induced by the DOXYL spin label are significant to determine longer distances over the whole range of the membrane depths. When these data were combined with estimated correlation times τc, the r-6-weighted, time-averaged distances between the spin-labels and the 31P nuclei on the membrane surface were estimated. The application of using this solid-state NMR PRE approach coupled with site-directed spin labeling (SDSL) may be a powerful method for measuring membrane protein immersion depth. © 2010 Elsevier Inc. All rights reserved.
KAUST Department:
Advanced Nanofabrication, Imaging and Characterization Core Lab
Publisher:
Elsevier
Journal:
Journal of Magnetic Resonance
Issue Date:
Nov-2010
DOI:
10.1016/j.jmr.2010.08.012
PubMed ID:
20851650
PubMed Central ID:
PMC2978330
Type:
Article
ISSN:
10907807
Sponsors:
This work was partially supported by the NIH Grant GM080542. The Bruker 500 MHz wide bore NMR spectrometer was obtained from the NSF Grant 10116333.
Additional Links:
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2978330
Appears in Collections:
Articles; Advanced Nanofabrication, Imaging and Characterization Core Lab

Full metadata record

DC FieldValue Language
dc.contributor.authorChu, Shidongen
dc.contributor.authorMaltsev, Sergey B.en
dc.contributor.authorEmwas, Abdul-Hamid M.en
dc.contributor.authorLorigan, Gary A.en
dc.date.accessioned2015-08-02T09:13:57Zen
dc.date.available2015-08-02T09:13:57Zen
dc.date.issued2010-11en
dc.identifier.issn10907807en
dc.identifier.pmid20851650en
dc.identifier.doi10.1016/j.jmr.2010.08.012en
dc.identifier.urihttp://hdl.handle.net/10754/561548en
dc.description.abstractA new approach for determining the membrane immersion depth of a spin-labeled probe has been developed using paramagnetic relaxation enhancement (PRE) in solid-state NMR spectroscopy. A DOXYL spin label was placed at different sites of 1-palmitoyl-2-stearoyl-sn-glycero-3-phosphocholine (PSPC) phospholipid bilayers as paramagnetic moieties and the resulting enhancements of the longitudinal relaxation (T1) times of 31P nuclei on the surface of the bilayers were measured by a standard inversion recovery pulse sequence. The 31P NMR spin-lattice relaxation times decrease steadily as the DOXYL spin label moves closer to the surface as well as the concentration of the spin-labeled lipids increase. The enhanced relaxation vs. the position and concentration of spin-labels indicate that PRE induced by the DOXYL spin label are significant to determine longer distances over the whole range of the membrane depths. When these data were combined with estimated correlation times τc, the r-6-weighted, time-averaged distances between the spin-labels and the 31P nuclei on the membrane surface were estimated. The application of using this solid-state NMR PRE approach coupled with site-directed spin labeling (SDSL) may be a powerful method for measuring membrane protein immersion depth. © 2010 Elsevier Inc. All rights reserved.en
dc.description.sponsorshipThis work was partially supported by the NIH Grant GM080542. The Bruker 500 MHz wide bore NMR spectrometer was obtained from the NSF Grant 10116333.en
dc.publisherElsevieren
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC2978330en
dc.subjectParamagnetic relaxation enhancementen
dc.subjectPhospholipid membranesen
dc.subjectSolid-state NMRen
dc.subjectSpin-lattice relaxationen
dc.titleSolid-state NMR paramagnetic relaxation enhancement immersion depth studies in phospholipid bilayersen
dc.typeArticleen
dc.contributor.departmentAdvanced Nanofabrication, Imaging and Characterization Core Laben
dc.identifier.journalJournal of Magnetic Resonanceen
dc.identifier.pmcidPMC2978330en
dc.contributor.institutionDepartment of Chemistry and Biochemistry, Miami University, Oxford, OH 45056, United Statesen
kaust.authorEmwas, Abdul-Hamid M.en
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