DNA replication factor C1 mediates genomic stability and transcriptional gene silencing in Arabidopsis

Handle URI:
http://hdl.handle.net/10754/561527
Title:
DNA replication factor C1 mediates genomic stability and transcriptional gene silencing in Arabidopsis
Authors:
Liu, Qian; Wang, Junguo; Miki, Daisuke; Xia, Ran; Yu, Wenxiang; He, Junna; Zheng, Zhimin; Zhu, Jian-Kang; Gonga, Zhizhong
Abstract:
Genetic screening identified a suppressor of ros1-1, a mutant of REPRESSOR OF SILENCING1 (ROS1; encoding a DNA demethylation protein). The suppressor is a mutation in the gene encoding the largest subunit of replication factor C (RFC1). This mutation of RFC1 reactivates the unlinked 35S-NPTII transgene, which is silenced in ros1 and also increases expression of the pericentromeric Athila retrotransposons named transcriptional silent information in a DNA methylationindependent manner. rfc1 is more sensitive than the wild type to the DNA-damaging agent methylmethane sulphonate and to the DNA inter- and intra- cross-linking agent cisplatin. The rfc1 mutant constitutively expresses the G2/M-specific cyclin CycB1;1 and other DNA repair-related genes. Treatment with DNA-damaging agents mimics the rfc1 mutation in releasing the silenced 35S-NPTII, suggesting that spontaneously induced genomic instability caused by the rfc1 mutation might partially contribute to the released transcriptional gene silencing (TGS). The frequency of somatic homologous recombination is significantly increased in the rfc1 mutant. Interestingly, ros1 mutants show increased telomere length, but rfc1 mutants show decreased telomere length and reduced expression of telomerase. Our results suggest that RFC1 helps mediate genomic stability and TGS in Arabidopsis thaliana. © 2010 American Society of Plant Biologists.
KAUST Department:
Center for Desert Agriculture
Publisher:
American Society of Plant Biologists (ASPB)
Journal:
THE PLANT CELL ONLINE
Issue Date:
1-Jul-2010
DOI:
10.1105/tpc.110.076349
PubMed ID:
20639449
PubMed Central ID:
PMC2929113
Type:
Article
ISSN:
10404651
Sponsors:
This work was supported by the National Nature Science Foundation of China (30630004 and 30721062), the National Transgenic Research Project (2008ZX08009-002), and the Program of Introducing Talents of Discipline to Universities (B06003) and Chinese Universities Scientific Fund to Z.G. We thank Yuelin Zhang for sharing the T-DNA lines provided by the ABRC (Columbus, OH).
Additional Links:
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2929113
Appears in Collections:
Articles; Center for Desert Agriculture

Full metadata record

DC FieldValue Language
dc.contributor.authorLiu, Qianen
dc.contributor.authorWang, Junguoen
dc.contributor.authorMiki, Daisukeen
dc.contributor.authorXia, Ranen
dc.contributor.authorYu, Wenxiangen
dc.contributor.authorHe, Junnaen
dc.contributor.authorZheng, Zhiminen
dc.contributor.authorZhu, Jian-Kangen
dc.contributor.authorGonga, Zhizhongen
dc.date.accessioned2015-08-02T09:13:30Zen
dc.date.available2015-08-02T09:13:30Zen
dc.date.issued2010-07-01en
dc.identifier.issn10404651en
dc.identifier.pmid20639449en
dc.identifier.doi10.1105/tpc.110.076349en
dc.identifier.urihttp://hdl.handle.net/10754/561527en
dc.description.abstractGenetic screening identified a suppressor of ros1-1, a mutant of REPRESSOR OF SILENCING1 (ROS1; encoding a DNA demethylation protein). The suppressor is a mutation in the gene encoding the largest subunit of replication factor C (RFC1). This mutation of RFC1 reactivates the unlinked 35S-NPTII transgene, which is silenced in ros1 and also increases expression of the pericentromeric Athila retrotransposons named transcriptional silent information in a DNA methylationindependent manner. rfc1 is more sensitive than the wild type to the DNA-damaging agent methylmethane sulphonate and to the DNA inter- and intra- cross-linking agent cisplatin. The rfc1 mutant constitutively expresses the G2/M-specific cyclin CycB1;1 and other DNA repair-related genes. Treatment with DNA-damaging agents mimics the rfc1 mutation in releasing the silenced 35S-NPTII, suggesting that spontaneously induced genomic instability caused by the rfc1 mutation might partially contribute to the released transcriptional gene silencing (TGS). The frequency of somatic homologous recombination is significantly increased in the rfc1 mutant. Interestingly, ros1 mutants show increased telomere length, but rfc1 mutants show decreased telomere length and reduced expression of telomerase. Our results suggest that RFC1 helps mediate genomic stability and TGS in Arabidopsis thaliana. © 2010 American Society of Plant Biologists.en
dc.description.sponsorshipThis work was supported by the National Nature Science Foundation of China (30630004 and 30721062), the National Transgenic Research Project (2008ZX08009-002), and the Program of Introducing Talents of Discipline to Universities (B06003) and Chinese Universities Scientific Fund to Z.G. We thank Yuelin Zhang for sharing the T-DNA lines provided by the ABRC (Columbus, OH).en
dc.publisherAmerican Society of Plant Biologists (ASPB)en
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC2929113en
dc.titleDNA replication factor C1 mediates genomic stability and transcriptional gene silencing in Arabidopsisen
dc.typeArticleen
dc.contributor.departmentCenter for Desert Agricultureen
dc.identifier.journalTHE PLANT CELL ONLINEen
dc.identifier.pmcidPMC2929113en
dc.contributor.institutionState Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, Chinaen
dc.contributor.institutionInstitute for Integrative Genome Biology, Department of Botany and Plant Sciences, University of California, Riverside, CA 92521, United Statesen
dc.contributor.institutionChina Agricultural University-University of California, Riverside Center for Biological Sciences and Biotechnology, Beijing 100193, Chinaen
dc.contributor.institutionNational Center for Plant Gene Research, Beijing 100193, Chinaen
kaust.authorZheng, Zhiminen
kaust.authorZhu, Jian-Kangen
kaust.authorMiki, Daisukeen

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