Microscopic structural analysis of fractured silk fibers from Bombyx mori and Samia cynthia ricini using 13C CP/MAS NMR with a 1 mm microcoil MAS NMR probehead

Handle URI:
http://hdl.handle.net/10754/561515
Title:
Microscopic structural analysis of fractured silk fibers from Bombyx mori and Samia cynthia ricini using 13C CP/MAS NMR with a 1 mm microcoil MAS NMR probehead
Authors:
Yamauchi, Kazuo; Yamasaki, Shizuo; Takahashi, Rui; Asakura, Tetsuo
Abstract:
Conformational changes have been studied in silk fibers from the domestic silkworm Bombyx mori and a wild silkworm Samia cynthia ricini as a result of fractured by stretching. About 300 samples consisting of only the fractured regions of [1-13C]Ala or [1-13C]Gly labeled silk fibers were collected and observed by 13C CP/MAS NMR spectra. The total amount of these fractured fibers is only about 1 mg and therefore we used a home-built 1 mm microcoil MAS NMR probehead. A very small increase in the fraction of random coil was noted for the alanine regions of both silk fibroins and for the glycine region of B. mori silk fibroin. However, there is no difference in the spectra before and after fractured for the glycine region of S. c. ricini silk fibroin. Thus, the influence of fracture occurs exclusively at the Ala region for S. c. ricini. The relationship between sequence, fracture and structure is discussed. © 2010 Elsevier Inc. All rights reserved.
KAUST Department:
Core Labs
Publisher:
Elsevier BV
Journal:
Solid State Nuclear Magnetic Resonance
Issue Date:
Jul-2010
DOI:
10.1016/j.ssnmr.2010.06.002
PubMed ID:
20579856
Type:
Article
ISSN:
09262040
Sponsors:
The authors acknowledge the financial support of SENTAN, JST for the NMR probehead developments. TA acknowledges support from a Grant-in-Aid for Scientific Research from Ministry of Education, Science, Culture and Supports of Japan (18105007) and also the Bio-oriented Technology Research Advancement Institution (BRAIN), Japan. The authors acknowledge Prof. Mike Williamson, University of Sheffield, UK for many suggestions and careful reading of our paper.
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Articles

Full metadata record

DC FieldValue Language
dc.contributor.authorYamauchi, Kazuoen
dc.contributor.authorYamasaki, Shizuoen
dc.contributor.authorTakahashi, Ruien
dc.contributor.authorAsakura, Tetsuoen
dc.date.accessioned2015-08-02T09:13:14Zen
dc.date.available2015-08-02T09:13:14Zen
dc.date.issued2010-07en
dc.identifier.issn09262040en
dc.identifier.pmid20579856en
dc.identifier.doi10.1016/j.ssnmr.2010.06.002en
dc.identifier.urihttp://hdl.handle.net/10754/561515en
dc.description.abstractConformational changes have been studied in silk fibers from the domestic silkworm Bombyx mori and a wild silkworm Samia cynthia ricini as a result of fractured by stretching. About 300 samples consisting of only the fractured regions of [1-13C]Ala or [1-13C]Gly labeled silk fibers were collected and observed by 13C CP/MAS NMR spectra. The total amount of these fractured fibers is only about 1 mg and therefore we used a home-built 1 mm microcoil MAS NMR probehead. A very small increase in the fraction of random coil was noted for the alanine regions of both silk fibroins and for the glycine region of B. mori silk fibroin. However, there is no difference in the spectra before and after fractured for the glycine region of S. c. ricini silk fibroin. Thus, the influence of fracture occurs exclusively at the Ala region for S. c. ricini. The relationship between sequence, fracture and structure is discussed. © 2010 Elsevier Inc. All rights reserved.en
dc.description.sponsorshipThe authors acknowledge the financial support of SENTAN, JST for the NMR probehead developments. TA acknowledges support from a Grant-in-Aid for Scientific Research from Ministry of Education, Science, Culture and Supports of Japan (18105007) and also the Bio-oriented Technology Research Advancement Institution (BRAIN), Japan. The authors acknowledge Prof. Mike Williamson, University of Sheffield, UK for many suggestions and careful reading of our paper.en
dc.publisherElsevier BVen
dc.subjectFiberen
dc.subjectMass-limited sampleen
dc.subjectProbeheaden
dc.subjectSilk fibroinen
dc.titleMicroscopic structural analysis of fractured silk fibers from Bombyx mori and Samia cynthia ricini using 13C CP/MAS NMR with a 1 mm microcoil MAS NMR probeheaden
dc.typeArticleen
dc.contributor.departmentCore Labsen
dc.identifier.journalSolid State Nuclear Magnetic Resonanceen
dc.contributor.institutionDepartment of Biotechnology, Tokyo University of Agriculture and Technology, Naka-cho 2-24-16, Koganei-shi, Tokyo 184-8588, Japanen
kaust.authorYamauchi, Kazuoen
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