Do GnRH analogues directly affect human endometrial epithelial cell gene expression?

Handle URI:
http://hdl.handle.net/10754/561483
Title:
Do GnRH analogues directly affect human endometrial epithelial cell gene expression?
Authors:
Zhang, Xiaomei; Bocca, Silvina Maria; Franchi, Anahí; Anderson, Sandra; Kaur, Mandeep; Bajic, Vladimir B. ( 0000-0001-5435-4750 ) ; Oehninger, Sergio Carlos
Abstract:
We examined whether Gonadotrophin-releasing hormone (GnRH) analogues [leuprolide acetate (LA) and ganirelix acetate (GA)] modulate gene expression in Ishikawa cells used as surrogate for human endometrial epithelial cells in vitro. The specific aims were: (i) to study the modulatory effect of GnRH analogues by RT-PCR [in the absence and presence of E2 and P4, and cyclic adenosine monophos-phate (cAMP)] on mRNA expression of genes modulated during the window of implantation in GnRH analogues/rFSH-treated assisted reproductive technology cycles including OPTINEURIN (OPTN), CHROMATIN MODIFYING PROTEIN (CHMP1A), PROSAPOSIN (PSAP), IGFBP-5 and SORTING NEXIN 7 (SNX7), and (ii) to analyze the 5'-flanking regions of such genes for the presence of putative steroid-response elements [estrogen-response elements (EREs) and P4-response element (PREs)]. Ishikawa cells were cytokeratin+/vimentin2 and expressed ERa,ERb, PR and GnRH-R proteins. At 6 and 24 h, neither LA nor GA alone had an effect on gene expression. GnRH analogues alone or following E2 and/or P4 co-incubation for 24 h also had no effect on gene expression, but P4 significantly increased expression of CHMP1A.E2 + P4 treatment for 4 days, alone or followed by GA, had no effect, but E2 + P4 treatment followed by LA significantly decreased IGFBP-5 expression. The addition of 8-Br cAMP did not modify gene expression, with the exception of IGFBP-5 that was significantly increased. The GnRH analogues did not modify intracellular cAMP levels. We identified conserved EREs for OPN, CHMP1A, SNX7 and PSAP and PREs for SNX7. We conclude that GnRH analogues appear not to have major direct effects on gene expression of human endo-metrial epithelial cells in vitro. © The Author 2010. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org.
KAUST Department:
Computational Bioscience Research Center (CBRC); Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division; Applied Mathematics and Computational Science Program
Publisher:
Oxford University Press (OUP)
Journal:
Molecular Human Reproduction
Issue Date:
4-Mar-2010
DOI:
10.1093/molehr/gaq012
PubMed ID:
20203127
Type:
Article
ISSN:
13609947
Sponsors:
Howard & Georgeanna Jones Foundation for Reproductive Medicine and Eastern Virginia Medical School.
Appears in Collections:
Articles; Applied Mathematics and Computational Science Program; Computational Bioscience Research Center (CBRC); Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorZhang, Xiaomeien
dc.contributor.authorBocca, Silvina Mariaen
dc.contributor.authorFranchi, Anahíen
dc.contributor.authorAnderson, Sandraen
dc.contributor.authorKaur, Mandeepen
dc.contributor.authorBajic, Vladimir B.en
dc.contributor.authorOehninger, Sergio Carlosen
dc.date.accessioned2015-08-02T09:12:29Zen
dc.date.available2015-08-02T09:12:29Zen
dc.date.issued2010-03-04en
dc.identifier.issn13609947en
dc.identifier.pmid20203127en
dc.identifier.doi10.1093/molehr/gaq012en
dc.identifier.urihttp://hdl.handle.net/10754/561483en
dc.description.abstractWe examined whether Gonadotrophin-releasing hormone (GnRH) analogues [leuprolide acetate (LA) and ganirelix acetate (GA)] modulate gene expression in Ishikawa cells used as surrogate for human endometrial epithelial cells in vitro. The specific aims were: (i) to study the modulatory effect of GnRH analogues by RT-PCR [in the absence and presence of E2 and P4, and cyclic adenosine monophos-phate (cAMP)] on mRNA expression of genes modulated during the window of implantation in GnRH analogues/rFSH-treated assisted reproductive technology cycles including OPTINEURIN (OPTN), CHROMATIN MODIFYING PROTEIN (CHMP1A), PROSAPOSIN (PSAP), IGFBP-5 and SORTING NEXIN 7 (SNX7), and (ii) to analyze the 5'-flanking regions of such genes for the presence of putative steroid-response elements [estrogen-response elements (EREs) and P4-response element (PREs)]. Ishikawa cells were cytokeratin+/vimentin2 and expressed ERa,ERb, PR and GnRH-R proteins. At 6 and 24 h, neither LA nor GA alone had an effect on gene expression. GnRH analogues alone or following E2 and/or P4 co-incubation for 24 h also had no effect on gene expression, but P4 significantly increased expression of CHMP1A.E2 + P4 treatment for 4 days, alone or followed by GA, had no effect, but E2 + P4 treatment followed by LA significantly decreased IGFBP-5 expression. The addition of 8-Br cAMP did not modify gene expression, with the exception of IGFBP-5 that was significantly increased. The GnRH analogues did not modify intracellular cAMP levels. We identified conserved EREs for OPN, CHMP1A, SNX7 and PSAP and PREs for SNX7. We conclude that GnRH analogues appear not to have major direct effects on gene expression of human endo-metrial epithelial cells in vitro. © The Author 2010. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org.en
dc.description.sponsorshipHoward & Georgeanna Jones Foundation for Reproductive Medicine and Eastern Virginia Medical School.en
dc.publisherOxford University Press (OUP)en
dc.subjectEndometriumen
dc.subjectEREs/PREs identificationen
dc.subjectGene expressionen
dc.subjectGnRHen
dc.subjectIshikawa cellsen
dc.titleDo GnRH analogues directly affect human endometrial epithelial cell gene expression?en
dc.typeArticleen
dc.contributor.departmentComputational Bioscience Research Center (CBRC)en
dc.contributor.departmentComputer, Electrical and Mathematical Sciences and Engineering (CEMSE) Divisionen
dc.contributor.departmentApplied Mathematics and Computational Science Programen
dc.identifier.journalMolecular Human Reproductionen
dc.contributor.institutionEastern Virginia Med Sch, Jones Inst Reprod Med, Dept Obstet & Gynecol, Norfolk, VA 23507 USAen
kaust.authorKaur, Mandeepen
kaust.authorBajic, Vladimir B.en
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