Development and heat stress-induced transcriptomic changes during embryogenesis of the scleractinian coral Acropora palmata

Handle URI:
http://hdl.handle.net/10754/561479
Title:
Development and heat stress-induced transcriptomic changes during embryogenesis of the scleractinian coral Acropora palmata
Authors:
Portune, Kevin J.; Voolstra, Christian R. ( 0000-0003-4555-3795 ) ; Medina, Mónica; Szmant, Alina M.
Abstract:
Projected elevation of seawater temperatures poses a threat to the reproductive success of Caribbean reef-building corals that have planktonic development during the warmest months of the year. This study examined the transcriptomic changes that occurred during embryonic and larval development of the elkhorn coral, Acropora palmata, at a non-stressful temperature (28 °C) and further assessed the effects of two elevated temperatures (30 °C and 31.5 °C) on these expression patterns. Using cDNA microarrays, we compared expression levels of 2051 genes from early embryos and larvae at multiple developmental stages (including pre-blastula, blastula, gastrula, and planula stages) at each of the three temperatures. At 12 h post-fertilization in 28 °C treatments, genes involved in cell replication/cell division and transcription were up-regulated in A. palmata embryos, followed by a reduction in expression of these genes during later growth stages. From 24.5 to 131 h post-fertilization at 28 °C, A. palmata altered its transcriptome by up-regulating genes involved in protein synthesis and metabolism. Temperatures of 30 °C and 31.5 °C caused major changes to the A. palmata embryonic transcriptomes, particularly in the samples from 24.5 hpf post-fertilization, characterized by down-regulation of numerous genes involved in cell replication/cell division, metabolism, cytoskeleton, and transcription, while heat shock genes were up-regulated compared to 28 °C treatments. These results suggest that increased temperature may cause a breakdown in proper gene expression during development in A. palmata by down-regulation of genes involved in essential cellular processes, which may lead to the abnormal development and reduced survivorship documented in other studies. © 2010 Elsevier B.V. All rights reserved.
KAUST Department:
Red Sea Research Center (RSRC); Biological and Environmental Sciences and Engineering (BESE) Division; Marine Science Program; Reef Genomics Lab
Publisher:
Elsevier BV
Journal:
Marine Genomics
Issue Date:
Mar-2010
DOI:
10.1016/j.margen.2010.03.002
PubMed ID:
21798197
Type:
Article
ISSN:
18747787
Sponsors:
We thank the Szmant lab (P. Erwin, K. Flynn, A. Miller, C. Randall, R. Whitehead) for help with the collection of Acropora spawn and larval culture. Dr. E. Weil of the University of Puerto Rico is thanked for helping us with access to his laboratory and facilitating field work. L Hiles did the characterization of the embryonic stages. We thank Drs. A. Wikramanayake and L Peshkin for suggestions on the manuscript. Support was provided by the World Bank funded Coral Reef Targeted Research Program, UNCW Academic Programs, and NSF grants BE-GEN 0313708 and IOS 0644438.
Appears in Collections:
Articles; Red Sea Research Center (RSRC); Marine Science Program; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorPortune, Kevin J.en
dc.contributor.authorVoolstra, Christian R.en
dc.contributor.authorMedina, Mónicaen
dc.contributor.authorSzmant, Alina M.en
dc.date.accessioned2015-08-02T09:12:24Zen
dc.date.available2015-08-02T09:12:24Zen
dc.date.issued2010-03en
dc.identifier.issn18747787en
dc.identifier.pmid21798197en
dc.identifier.doi10.1016/j.margen.2010.03.002en
dc.identifier.urihttp://hdl.handle.net/10754/561479en
dc.description.abstractProjected elevation of seawater temperatures poses a threat to the reproductive success of Caribbean reef-building corals that have planktonic development during the warmest months of the year. This study examined the transcriptomic changes that occurred during embryonic and larval development of the elkhorn coral, Acropora palmata, at a non-stressful temperature (28 °C) and further assessed the effects of two elevated temperatures (30 °C and 31.5 °C) on these expression patterns. Using cDNA microarrays, we compared expression levels of 2051 genes from early embryos and larvae at multiple developmental stages (including pre-blastula, blastula, gastrula, and planula stages) at each of the three temperatures. At 12 h post-fertilization in 28 °C treatments, genes involved in cell replication/cell division and transcription were up-regulated in A. palmata embryos, followed by a reduction in expression of these genes during later growth stages. From 24.5 to 131 h post-fertilization at 28 °C, A. palmata altered its transcriptome by up-regulating genes involved in protein synthesis and metabolism. Temperatures of 30 °C and 31.5 °C caused major changes to the A. palmata embryonic transcriptomes, particularly in the samples from 24.5 hpf post-fertilization, characterized by down-regulation of numerous genes involved in cell replication/cell division, metabolism, cytoskeleton, and transcription, while heat shock genes were up-regulated compared to 28 °C treatments. These results suggest that increased temperature may cause a breakdown in proper gene expression during development in A. palmata by down-regulation of genes involved in essential cellular processes, which may lead to the abnormal development and reduced survivorship documented in other studies. © 2010 Elsevier B.V. All rights reserved.en
dc.description.sponsorshipWe thank the Szmant lab (P. Erwin, K. Flynn, A. Miller, C. Randall, R. Whitehead) for help with the collection of Acropora spawn and larval culture. Dr. E. Weil of the University of Puerto Rico is thanked for helping us with access to his laboratory and facilitating field work. L Hiles did the characterization of the embryonic stages. We thank Drs. A. Wikramanayake and L Peshkin for suggestions on the manuscript. Support was provided by the World Bank funded Coral Reef Targeted Research Program, UNCW Academic Programs, and NSF grants BE-GEN 0313708 and IOS 0644438.en
dc.publisherElsevier BVen
dc.subjectCoralen
dc.subjectLarval developmenten
dc.subjectMicroarrayen
dc.subjectTemperature stressen
dc.titleDevelopment and heat stress-induced transcriptomic changes during embryogenesis of the scleractinian coral Acropora palmataen
dc.typeArticleen
dc.contributor.departmentRed Sea Research Center (RSRC)en
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentMarine Science Programen
dc.contributor.departmentReef Genomics Laben
dc.identifier.journalMarine Genomicsen
dc.contributor.institutionCenter for Marine Science, University North Carolina Wilmington, 5600 Marvin K. Moss Lane, Wilmington, NC 28409, United Statesen
dc.contributor.institutionSchool of Natural Sciences, University of California, Merced, PO Box 2039, Merced, CA 95344, United Statesen
kaust.authorVoolstra, Christian R.en

Related articles on PubMed

All Items in KAUST are protected by copyright, with all rights reserved, unless otherwise indicated.