An SGS3-like protein functions in RNA-directed DNA methylation and transcriptional gene silencing in Arabidopsis

Handle URI:
http://hdl.handle.net/10754/561468
Title:
An SGS3-like protein functions in RNA-directed DNA methylation and transcriptional gene silencing in Arabidopsis
Authors:
Zheng, Zhimin; Xing, Yu; He, Xinjian; Li, Wenbo; Hu, Yuanlei; Yadav, Sudesh Kumar; Oh, Jeeeun; Zhu, Jian-Kang
Abstract:
RNA-directed DNA methylation (RdDM) is an important epigenetic mechanism for silencing transgenes and endogenous repetitive sequences such as transposons. The RD29A promoter-driven LUCIFERASE transgene and its corresponding endogenous RD29A gene are hypermethylated and silenced in the Arabidopsis DNA demethylase mutant ros1. By screening for second-site suppressors of ros1, we identified the RDM12 locus. The rdm12 mutation releases the silencing of the RD29A-LUC transgene and the endogenous RD29A gene by reducing the promoter DNA methylation. The rdm12 mutation also reduces DNA methylation at endogenous RdDM target loci, including transposons and other repetitive sequences. In addition, the rdm12 mutation affects the levels of small interfering RNAs (siRNAs) from some of the RdDM target loci. RDM12 encodes a protein with XS and coiled-coil domains, and is similar to SGS3, which is a partner protein of RDR6 and can bind to double-stranded RNAs with a 5′ overhang, and is required for several post-transcriptional gene silencing pathways. Our results show that RDM12 is a component of the RdDM pathway, and suggest that RdDM may involve double-stranded RNAs with a 5′ overhang and the partnering between RDM12 and RDR2. © 2010 Blackwell Publishing Ltd.
KAUST Department:
Center for Desert Agriculture
Publisher:
Wiley-Blackwell
Journal:
The Plant Journal
Issue Date:
6-Jan-2010
DOI:
10.1111/j.1365-313X.2010.04130.x
PubMed ID:
20059743
PubMed Central ID:
PMC2858770
Type:
Article
ISSN:
09607412
Sponsors:
This work was supported by National Institutes of Health grant R01GM070795 to J-KZ and a BOYSCAST Fellowship, Department of Science and Technology, Government of India to SKY.
Additional Links:
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2858770
Appears in Collections:
Articles; Center for Desert Agriculture

Full metadata record

DC FieldValue Language
dc.contributor.authorZheng, Zhiminen
dc.contributor.authorXing, Yuen
dc.contributor.authorHe, Xinjianen
dc.contributor.authorLi, Wenboen
dc.contributor.authorHu, Yuanleien
dc.contributor.authorYadav, Sudesh Kumaren
dc.contributor.authorOh, Jeeeunen
dc.contributor.authorZhu, Jian-Kangen
dc.date.accessioned2015-08-02T09:12:08Zen
dc.date.available2015-08-02T09:12:08Zen
dc.date.issued2010-01-06en
dc.identifier.issn09607412en
dc.identifier.pmid20059743en
dc.identifier.doi10.1111/j.1365-313X.2010.04130.xen
dc.identifier.urihttp://hdl.handle.net/10754/561468en
dc.description.abstractRNA-directed DNA methylation (RdDM) is an important epigenetic mechanism for silencing transgenes and endogenous repetitive sequences such as transposons. The RD29A promoter-driven LUCIFERASE transgene and its corresponding endogenous RD29A gene are hypermethylated and silenced in the Arabidopsis DNA demethylase mutant ros1. By screening for second-site suppressors of ros1, we identified the RDM12 locus. The rdm12 mutation releases the silencing of the RD29A-LUC transgene and the endogenous RD29A gene by reducing the promoter DNA methylation. The rdm12 mutation also reduces DNA methylation at endogenous RdDM target loci, including transposons and other repetitive sequences. In addition, the rdm12 mutation affects the levels of small interfering RNAs (siRNAs) from some of the RdDM target loci. RDM12 encodes a protein with XS and coiled-coil domains, and is similar to SGS3, which is a partner protein of RDR6 and can bind to double-stranded RNAs with a 5′ overhang, and is required for several post-transcriptional gene silencing pathways. Our results show that RDM12 is a component of the RdDM pathway, and suggest that RdDM may involve double-stranded RNAs with a 5′ overhang and the partnering between RDM12 and RDR2. © 2010 Blackwell Publishing Ltd.en
dc.description.sponsorshipThis work was supported by National Institutes of Health grant R01GM070795 to J-KZ and a BOYSCAST Fellowship, Department of Science and Technology, Government of India to SKY.en
dc.publisherWiley-Blackwellen
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC2858770en
dc.subjectDNA methylationen
dc.subjectEpigeneticsen
dc.subjectRDM12en
dc.subjectSGS3en
dc.subjectSiRNAen
dc.titleAn SGS3-like protein functions in RNA-directed DNA methylation and transcriptional gene silencing in Arabidopsisen
dc.typeArticleen
dc.contributor.departmentCenter for Desert Agricultureen
dc.identifier.journalThe Plant Journalen
dc.identifier.pmcidPMC2858770en
dc.contributor.institutionInstitute for Integrative Genome Biology, Department of Botany and Plant Sciences, University of California, Riverside, CA 92521, United Statesen
dc.contributor.institutionKey Laboratory of Plant Pathology, Department of Plant Pathology, China Agricultural University, Yuanmingyuan West Road 2, Beijing 100094, Chinaen
dc.contributor.institutionCollege of Life Sciences, Beijing Normal University, Beijing 100875, Chinaen
dc.contributor.institutionBiotechnology Division, Institute of Himalayan Bioresource Technology, CSIR, Palampur-176061 (HP), Indiaen
kaust.authorZheng, Zhiminen
kaust.authorXing, Yuen
kaust.authorHe, Xinjianen
kaust.authorZhu, Jian-Kangen

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