RAS1, a quantitative trait locus for salt tolerance and ABA sensitivity in Arabidopsis

Handle URI:
http://hdl.handle.net/10754/561464
Title:
RAS1, a quantitative trait locus for salt tolerance and ABA sensitivity in Arabidopsis
Authors:
Ren, Zhonghai; Zheng, Zhimin; Chinnusamy, Viswanathan; Zhu, Jianhua; Cui, Xinping; Iida, Kei; Zhu, Jian-Kang
Abstract:
Soil salinity limits agricultural production and is a major obstacle for feeding the growing world population. We used natural genetic variation in salt tolerance among different Arabidopsis accessions to map a major quantitative trait locus (QTL) for salt tolerance and abscisic acid (ABA) sensitivity during seed germination and early seedling growth. A recombinant inbred population derived from Landsberg erecta (Ler; salt and ABA sensitive) x Shakdara (Sha; salt and ABA resistant) was used for QTL mapping. High-resolution mapping and cloning of this QTL, Response to ABA and Salt 1 (RAS1), revealed that it is an ABA- and salt stress-inducible gene and encodes a previously undescribed plant-specific protein. A premature stop codon results in a truncated RAS1 protein in Sha. Reducing the expression of RAS1 by transfer-DNA insertion in Col or RNA interference in Ler leads to decreased salt and ABA sensitivity, whereas overexpression of the Ler allele but not the Sha allele causes increased salt and ABA sensitivity. Our results suggest that RAS1 functions as a negative regulator of salt tolerance during seed germination and early seedling growth by enhancing ABA sensitivity and that its loss of function contributes to the increased salt tolerance of Sha.
KAUST Department:
Center for Desert Agriculture
Publisher:
Proceedings of the National Academy of Sciences
Journal:
Proceedings of the National Academy of Sciences
Issue Date:
8-Mar-2010
DOI:
10.1073/pnas.0910798107
PubMed ID:
20212128
PubMed Central ID:
PMC2851765
Type:
Article
ISSN:
00278424
Sponsors:
We thank Maarten Koornneef for kindly providing the Ler x Sha RIL population and Hong-Xuan Lin for valuable advice on QTL analysis. This work was supported by National Institutes of Health Grants R01GM070795 and R01GM059138 and by National Science Foundation Grant IBN0420152 ( to J.-K.Z). Z.Z. was supported by the China Scholarship Council.
Additional Links:
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2851765
Appears in Collections:
Articles; Center for Desert Agriculture

Full metadata record

DC FieldValue Language
dc.contributor.authorRen, Zhonghaien
dc.contributor.authorZheng, Zhiminen
dc.contributor.authorChinnusamy, Viswanathanen
dc.contributor.authorZhu, Jianhuaen
dc.contributor.authorCui, Xinpingen
dc.contributor.authorIida, Keien
dc.contributor.authorZhu, Jian-Kangen
dc.date.accessioned2015-08-02T09:12:02Zen
dc.date.available2015-08-02T09:12:02Zen
dc.date.issued2010-03-08en
dc.identifier.issn00278424en
dc.identifier.pmid20212128en
dc.identifier.doi10.1073/pnas.0910798107en
dc.identifier.urihttp://hdl.handle.net/10754/561464en
dc.description.abstractSoil salinity limits agricultural production and is a major obstacle for feeding the growing world population. We used natural genetic variation in salt tolerance among different Arabidopsis accessions to map a major quantitative trait locus (QTL) for salt tolerance and abscisic acid (ABA) sensitivity during seed germination and early seedling growth. A recombinant inbred population derived from Landsberg erecta (Ler; salt and ABA sensitive) x Shakdara (Sha; salt and ABA resistant) was used for QTL mapping. High-resolution mapping and cloning of this QTL, Response to ABA and Salt 1 (RAS1), revealed that it is an ABA- and salt stress-inducible gene and encodes a previously undescribed plant-specific protein. A premature stop codon results in a truncated RAS1 protein in Sha. Reducing the expression of RAS1 by transfer-DNA insertion in Col or RNA interference in Ler leads to decreased salt and ABA sensitivity, whereas overexpression of the Ler allele but not the Sha allele causes increased salt and ABA sensitivity. Our results suggest that RAS1 functions as a negative regulator of salt tolerance during seed germination and early seedling growth by enhancing ABA sensitivity and that its loss of function contributes to the increased salt tolerance of Sha.en
dc.description.sponsorshipWe thank Maarten Koornneef for kindly providing the Ler x Sha RIL population and Hong-Xuan Lin for valuable advice on QTL analysis. This work was supported by National Institutes of Health Grants R01GM070795 and R01GM059138 and by National Science Foundation Grant IBN0420152 ( to J.-K.Z). Z.Z. was supported by the China Scholarship Council.en
dc.publisherProceedings of the National Academy of Sciencesen
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC2851765en
dc.subjectAbscisic acid responseen
dc.subjectGerminationen
dc.subjectNatural variationen
dc.subjectQuantitative trait locusen
dc.titleRAS1, a quantitative trait locus for salt tolerance and ABA sensitivity in Arabidopsisen
dc.typeArticleen
dc.contributor.departmentCenter for Desert Agricultureen
dc.identifier.journalProceedings of the National Academy of Sciencesen
dc.identifier.pmcidPMC2851765en
dc.contributor.institutionCenter for Plant Cell Biology, Institute for Integrative Genome Biology, University of California, Riverside, CA 92521, United Statesen
dc.contributor.institutionKey Laboratory of Plant Pathology, Department of Plant Pathology, China Agricultural University, Beijing 100094, Chinaen
dc.contributor.institutionDepartment of Statistics, University of California, Riverside, CA 92521, United Statesen
dc.contributor.institutionDepartment of Plant Science and Landscape Architecture, University of Maryland, College Park, MD 20742, United Statesen
dc.contributor.institutionCollege of Horticulture Science and Engineering, Shandong Agricultural University, Taian, Shandong 271018, Chinaen
kaust.authorZheng, Zhiminen
kaust.authorChinnusamy, Viswanathanen
kaust.authorZhu, Jian-Kangen

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