Coral host transcriptomic states are correlated with Symbiodinium genotypes

Handle URI:
http://hdl.handle.net/10754/561462
Title:
Coral host transcriptomic states are correlated with Symbiodinium genotypes
Authors:
DeSalvo, Michael K.; Sunagawa, Shinichi; Fisher, Paul L.; Voolstra, Christian R. ( 0000-0003-4555-3795 ) ; Iglesias Prieto, Roberto; Medina, Mónica
Abstract:
A mutualistic relationship between reef-building corals and endosymbiotic dinoflagellates (Symbiodinium spp.) forms the basis for the existence of coral reefs. Genotyping tools for Symbiodinium spp. have added a new level of complexity to studies concerning cnidarian growth, nutrient acquisition, and stress. For example, the response of the coral holobiont to thermal stress is connected to the host-Symbiodinium genotypic combination, as different partnerships can have different bleaching susceptibilities. In this study, we monitored Symbiodinium physiological parameters and profiled the coral host transcriptional responses in acclimated, thermally stressed, and recovered fragments of the coral Montastraea faveolata using a custom cDNA gene expression microarray. Interestingly, gene expression was more similar among samples with the same Symbiodinium content rather than the same experimental condition. In order to discount for host-genotypic effects, we sampled fragments from a single colony of M. faveolata containing different symbiont types, and found that the host transcriptomic states grouped according to Symbiodinium genotype rather than thermal stress. As the first study that links coral host transcriptomic patterns to the clade content of their Symbiodinium community, our results provide a critical step to elucidating the molecular basis of the apparent variability seen among different coral-Symbiodinium partnerships. © 2010 Blackwell Publishing Ltd.
KAUST Department:
Red Sea Research Center (RSRC); Biological and Environmental Sciences and Engineering (BESE) Division; Marine Science Program; Reef Genomics Lab
Publisher:
Wiley
Journal:
Molecular Ecology
Issue Date:
Mar-2010
DOI:
10.1111/j.1365-294X.2010.04534.x
PubMed ID:
20149089
Type:
Article
ISSN:
09621083
Sponsors:
We would like to thank the members of the Medina Lab at UCM and the members of the Photobiology group in Puerto Morelos for aid in many aspects of this study. We would like to especially thank Xavier Hernandez-Pech of UNAM, Erika Diaz-Almeyda of UCM, Keith Bayha of UCM, Lolo Cardenas of the Roy Genomics Shared Facility at UCM, and the Functional Genomics Lab at UC Berkeley. Nicholas Polato and Iliana Baums at Penn State University are thanked for genotyping coral colonies. The comments of anonymous reviewers greatly improved this manuscript. This study was supported by a UC MEXUS Dissertation Research Grant to MKD, two Sigma Xi Grants-in-Aid of Research to MKD and SS, a UC MEXUS-CONACYT Collaborative Grant to RI-P and MM, NSF awards to MM (BE-GEN 0313708 and IOS 0644438), start- up funds from UC Merced to MM, and Coral Reef Targeted Research Program Funds to RI-P.
Appears in Collections:
Articles; Red Sea Research Center (RSRC); Marine Science Program; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorDeSalvo, Michael K.en
dc.contributor.authorSunagawa, Shinichien
dc.contributor.authorFisher, Paul L.en
dc.contributor.authorVoolstra, Christian R.en
dc.contributor.authorIglesias Prieto, Robertoen
dc.contributor.authorMedina, Mónicaen
dc.date.accessioned2015-08-02T09:11:59Zen
dc.date.available2015-08-02T09:11:59Zen
dc.date.issued2010-03en
dc.identifier.issn09621083en
dc.identifier.pmid20149089en
dc.identifier.doi10.1111/j.1365-294X.2010.04534.xen
dc.identifier.urihttp://hdl.handle.net/10754/561462en
dc.description.abstractA mutualistic relationship between reef-building corals and endosymbiotic dinoflagellates (Symbiodinium spp.) forms the basis for the existence of coral reefs. Genotyping tools for Symbiodinium spp. have added a new level of complexity to studies concerning cnidarian growth, nutrient acquisition, and stress. For example, the response of the coral holobiont to thermal stress is connected to the host-Symbiodinium genotypic combination, as different partnerships can have different bleaching susceptibilities. In this study, we monitored Symbiodinium physiological parameters and profiled the coral host transcriptional responses in acclimated, thermally stressed, and recovered fragments of the coral Montastraea faveolata using a custom cDNA gene expression microarray. Interestingly, gene expression was more similar among samples with the same Symbiodinium content rather than the same experimental condition. In order to discount for host-genotypic effects, we sampled fragments from a single colony of M. faveolata containing different symbiont types, and found that the host transcriptomic states grouped according to Symbiodinium genotype rather than thermal stress. As the first study that links coral host transcriptomic patterns to the clade content of their Symbiodinium community, our results provide a critical step to elucidating the molecular basis of the apparent variability seen among different coral-Symbiodinium partnerships. © 2010 Blackwell Publishing Ltd.en
dc.description.sponsorshipWe would like to thank the members of the Medina Lab at UCM and the members of the Photobiology group in Puerto Morelos for aid in many aspects of this study. We would like to especially thank Xavier Hernandez-Pech of UNAM, Erika Diaz-Almeyda of UCM, Keith Bayha of UCM, Lolo Cardenas of the Roy Genomics Shared Facility at UCM, and the Functional Genomics Lab at UC Berkeley. Nicholas Polato and Iliana Baums at Penn State University are thanked for genotyping coral colonies. The comments of anonymous reviewers greatly improved this manuscript. This study was supported by a UC MEXUS Dissertation Research Grant to MKD, two Sigma Xi Grants-in-Aid of Research to MKD and SS, a UC MEXUS-CONACYT Collaborative Grant to RI-P and MM, NSF awards to MM (BE-GEN 0313708 and IOS 0644438), start- up funds from UC Merced to MM, and Coral Reef Targeted Research Program Funds to RI-P.en
dc.publisherWileyen
dc.subjectCoral bleachingen
dc.subjectGene expressionen
dc.subjectMicroarrayen
dc.subjectSymbiodiniumen
dc.subjectThermal stressen
dc.titleCoral host transcriptomic states are correlated with Symbiodinium genotypesen
dc.typeArticleen
dc.contributor.departmentRed Sea Research Center (RSRC)en
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentMarine Science Programen
dc.contributor.departmentReef Genomics Laben
dc.identifier.journalMolecular Ecologyen
dc.contributor.institutionSchool of Natural Sciences, University of California, Merced, 5200 N. Lake Road, Merced, CA 95343, United Statesen
dc.contributor.institutionUnidad Académica Puerto Morelos, Instituto de Ciencias del Mar y Limnología, Universidad Nacional Autónoma de México, Apartado Postal 1152, Cancún 77500 QR, Mexicoen
kaust.authorVoolstra, Christian R.en
kaust.authorFisher, Paul L.en

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