RoboSCell: An automated single cell arraying and analysis instrument

Handle URI:
http://hdl.handle.net/10754/561431
Title:
RoboSCell: An automated single cell arraying and analysis instrument
Authors:
Sakaki, Kelly; Foulds, Ian G.; Liu, William; Dechev, Nikolai; Burke, Robert Douglas; Park, Edward
Abstract:
Single cell research has the potential to revolutionize experimental methods in biomedical sciences and contribute to clinical practices. Recent studies suggest analysis of single cells reveals novel features of intracellular processes, cell-to-cell interactions and cell structure. The methods of single cell analysis require mechanical resolution and accuracy that is not possible using conventional techniques. Robotic instruments and novel microdevices can achieve higher throughput and repeatability; however, the development of such instrumentation is a formidable task. A void exists in the state-of-the-art for automated analysis of single cells. With the increase in interest in single cell analyses in stem cell and cancer research the ability to facilitate higher throughput and repeatable procedures is necessary. In this paper, a high-throughput, single cell microarray-based robotic instrument, called the RoboSCell, is described. The proposed instrument employs a partially transparent single cell microarray (SCM) integrated with a robotic biomanipulator for in vitro analyses of live single cells trapped at the array sites. Cells, labeled with immunomagnetic particles, are captured at the array sites by channeling magnetic fields through encapsulated permalloy channels in the SCM. The RoboSCell is capable of systematically scanning the captured cells temporarily immobilized at the array sites and using optical methods to repeatedly measure extracellular and intracellular characteristics over time. The instrument's capabilities are demonstrated by arraying human T lymphocytes and measuring the uptake dynamics of calcein acetoxymethylester-all in a fully automated fashion. © 2009 Springer Science+Business Media, LLC.
KAUST Department:
Electrical Engineering Program; Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
Publisher:
Springer Verlag
Journal:
Biomedical Microdevices
Issue Date:
9-Sep-2009
DOI:
10.1007/s10544-009-9351-3
PubMed ID:
19763834
Type:
Article
ISSN:
13872176
Appears in Collections:
Articles; Electrical Engineering Program; Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorSakaki, Kellyen
dc.contributor.authorFoulds, Ian G.en
dc.contributor.authorLiu, Williamen
dc.contributor.authorDechev, Nikolaien
dc.contributor.authorBurke, Robert Douglasen
dc.contributor.authorPark, Edwarden
dc.date.accessioned2015-08-02T09:11:07Zen
dc.date.available2015-08-02T09:11:07Zen
dc.date.issued2009-09-09en
dc.identifier.issn13872176en
dc.identifier.pmid19763834en
dc.identifier.doi10.1007/s10544-009-9351-3en
dc.identifier.urihttp://hdl.handle.net/10754/561431en
dc.description.abstractSingle cell research has the potential to revolutionize experimental methods in biomedical sciences and contribute to clinical practices. Recent studies suggest analysis of single cells reveals novel features of intracellular processes, cell-to-cell interactions and cell structure. The methods of single cell analysis require mechanical resolution and accuracy that is not possible using conventional techniques. Robotic instruments and novel microdevices can achieve higher throughput and repeatability; however, the development of such instrumentation is a formidable task. A void exists in the state-of-the-art for automated analysis of single cells. With the increase in interest in single cell analyses in stem cell and cancer research the ability to facilitate higher throughput and repeatable procedures is necessary. In this paper, a high-throughput, single cell microarray-based robotic instrument, called the RoboSCell, is described. The proposed instrument employs a partially transparent single cell microarray (SCM) integrated with a robotic biomanipulator for in vitro analyses of live single cells trapped at the array sites. Cells, labeled with immunomagnetic particles, are captured at the array sites by channeling magnetic fields through encapsulated permalloy channels in the SCM. The RoboSCell is capable of systematically scanning the captured cells temporarily immobilized at the array sites and using optical methods to repeatedly measure extracellular and intracellular characteristics over time. The instrument's capabilities are demonstrated by arraying human T lymphocytes and measuring the uptake dynamics of calcein acetoxymethylester-all in a fully automated fashion. © 2009 Springer Science+Business Media, LLC.en
dc.publisherSpringer Verlagen
dc.subjectAutomationen
dc.subjectImmunomagneticen
dc.subjectInstrumentationen
dc.subjectManipulationen
dc.subjectMEMSen
dc.subjectMicroarrayen
dc.subjectSingle cellen
dc.titleRoboSCell: An automated single cell arraying and analysis instrumenten
dc.typeArticleen
dc.contributor.departmentElectrical Engineering Programen
dc.contributor.departmentComputer, Electrical and Mathematical Sciences and Engineering (CEMSE) Divisionen
dc.identifier.journalBiomedical Microdevicesen
dc.contributor.institutionMechatronic Systems Engineering, School of Engineering Science, Simon Fraser University, Central City Galleria 4 4274, Surrey, BC V3T 0A3, Canadaen
dc.contributor.institutionDepartment of Mechanical Engineering, University of Victoria, STN CSC, Victoria, BC V8W 3P6, Canadaen
dc.contributor.institutionDepartment of Biochemistry and Microbiology, University of Victoria, STN CSC, Victoria, BC V8W 3P6, Canadaen
kaust.authorFoulds, Ian G.en

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