Seasonality in molecular and cytometric diversity of marine bacterioplankton: the reshuffling of bacterial taxa by vertical mixing

Handle URI:
http://hdl.handle.net/10754/561069
Title:
Seasonality in molecular and cytometric diversity of marine bacterioplankton: the reshuffling of bacterial taxa by vertical mixing
Authors:
García, Francisca C.; Alonso-Sáez, Laura; Moran, Xose Anxelu G. ( 0000-0002-9823-5339 ) ; López-Urrutia, Ángel
Abstract:
The ’cytometric diversity’ of phytoplankton communities has been studied based on single-cell properties, but the applicability of this method to characterize bacterioplankton has been unexplored. Here, we analysed seasonal changes in cytometric diversity of marine bacterioplankton along a decadal time-series at three coastal stations in the Southern Bay of Biscay. Shannon-Weaver diversity estimates and Bray-Curtis similarities obtained by cytometric and molecular (16S rRNA tag sequencing) methods were significantly correlated in samples from a 3.5-year monthly time-series. Both methods showed a consistent cyclical pattern in the diversity of surface bacterial communities with maximal values in winter. The analysis of the highly resolved flow cytometry time-series across the vertical profile showed that water column mixing was a key factor explaining the seasonal changes in bacterial composition and the winter increase in bacterial diversity in coastal surface waters. Due to its low cost and short processing time as compared to genetic methods, the cytometric diversity approach represents a useful complementary tool in the macroecology of aquatic microbes.
KAUST Department:
Red Sea Research Center (RSRC); Biological and Environmental Sciences and Engineering (BESE) Division
Citation:
Seasonality in molecular and cytometric diversity of marine bacterioplankton: the reshuffling of bacterial taxa by vertical mixing 2015:n/a Environmental Microbiology
Journal:
Environmental Microbiology
Issue Date:
17-Jul-2015
DOI:
10.1111/1462-2920.12984
Type:
Article
ISSN:
14622912
Additional Links:
http://doi.wiley.com/10.1111/1462-2920.12984
Appears in Collections:
Articles; Red Sea Research Center (RSRC); Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorGarcía, Francisca C.en
dc.contributor.authorAlonso-Sáez, Lauraen
dc.contributor.authorMoran, Xose Anxelu G.en
dc.contributor.authorLópez-Urrutia, Ángelen
dc.date.accessioned2015-07-27T12:27:08Zen
dc.date.available2015-07-27T12:27:08Zen
dc.date.issued2015-07-17en
dc.identifier.citationSeasonality in molecular and cytometric diversity of marine bacterioplankton: the reshuffling of bacterial taxa by vertical mixing 2015:n/a Environmental Microbiologyen
dc.identifier.issn14622912en
dc.identifier.doi10.1111/1462-2920.12984en
dc.identifier.urihttp://hdl.handle.net/10754/561069en
dc.description.abstractThe ’cytometric diversity’ of phytoplankton communities has been studied based on single-cell properties, but the applicability of this method to characterize bacterioplankton has been unexplored. Here, we analysed seasonal changes in cytometric diversity of marine bacterioplankton along a decadal time-series at three coastal stations in the Southern Bay of Biscay. Shannon-Weaver diversity estimates and Bray-Curtis similarities obtained by cytometric and molecular (16S rRNA tag sequencing) methods were significantly correlated in samples from a 3.5-year monthly time-series. Both methods showed a consistent cyclical pattern in the diversity of surface bacterial communities with maximal values in winter. The analysis of the highly resolved flow cytometry time-series across the vertical profile showed that water column mixing was a key factor explaining the seasonal changes in bacterial composition and the winter increase in bacterial diversity in coastal surface waters. Due to its low cost and short processing time as compared to genetic methods, the cytometric diversity approach represents a useful complementary tool in the macroecology of aquatic microbes.en
dc.relation.urlhttp://doi.wiley.com/10.1111/1462-2920.12984en
dc.rightsThis is the peer reviewed version of the following article: García, Francisca C., Laura Alonso‐Sáez, Xosé Anxelu G. Morán, and Ángel López‐Urrutia. "Seasonality in molecular and cytometric diversity of marine bacterioplankton: the reshuffling of bacterial taxa by vertical mixing." Environmental Microbiology (2015), which has been published in final form at http://doi.wiley.com/10.1111/1462-2920.12984. This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.en
dc.titleSeasonality in molecular and cytometric diversity of marine bacterioplankton: the reshuffling of bacterial taxa by vertical mixingen
dc.typeArticleen
dc.contributor.departmentRed Sea Research Center (RSRC)en
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalEnvironmental Microbiologyen
dc.eprint.versionPost-printen
dc.contributor.institutionInstituto Español de Oceanografía, Centro Oceanográfico de Gijón/Xixón, 33212 Gijón/Xixón; Spainen
dc.contributor.institutionAZTI-Tecnalia, Marine Research Division, Txatxarramendi Irla, s/n. 48395 Sukarrieta, Spainen
kaust.authorMoran, Xose Anxelu G.en
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