Functionalized PDMS with versatile and scalable surface roughness gradients for cell culture

Handle URI:
http://hdl.handle.net/10754/561062
Title:
Functionalized PDMS with versatile and scalable surface roughness gradients for cell culture
Authors:
Zhou, Bingpu; Gao, Xinghua; Wang, Cong; Ye, Ziran; Gao, Yibo; Xie, Jiao; Wu, Xiaoxiao; Wen, Weijia
Abstract:
This manuscript describes a simple and versatile approach to engineering surface roughness gradients via combination of microfluidics and photo-polymerization. Through UV-mediated polymerization, N-isopropylacrylamide with concentration gradients are successfully grafted onto PDMS surface, leading to diverse roughness degrees on the obtained PDMS substrate. Furthermore, the extent of surface roughness can be controllably regulated via tuning the flow rate ratio between the monomer solution and deionized water. Average roughness ranging from 8.050 nm to 151.68 nm has well been achieved in this work. Such PDMS samples are also demonstrated to be capable of working as supporting substrates for controlling cell adhesion or detachment. Due to the different degrees of surface roughness on a single substrate, our method provides an effective approach for designing advanced surafecs for cell culture. Finally, the thermosensitive property of N-isopropylacrylamide makes our sample furnish as another means for controlling the cell detachment from the substrates with correspondence to the surrounding temperature.
Citation:
Functionalized PDMS with versatile and scalable surface roughness gradients for cell culture 2015:150721053642001 ACS Applied Materials & Interfaces
Journal:
ACS Applied Materials & Interfaces
Issue Date:
21-Jul-2015
DOI:
10.1021/acsami.5b04032
Type:
Article
ISSN:
1944-8244; 1944-8252
Additional Links:
http://pubs.acs.org/doi/abs/10.1021/acsami.5b04032
Appears in Collections:
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Full metadata record

DC FieldValue Language
dc.contributor.authorZhou, Bingpuen
dc.contributor.authorGao, Xinghuaen
dc.contributor.authorWang, Congen
dc.contributor.authorYe, Ziranen
dc.contributor.authorGao, Yiboen
dc.contributor.authorXie, Jiaoen
dc.contributor.authorWu, Xiaoxiaoen
dc.contributor.authorWen, Weijiaen
dc.date.accessioned2015-07-27T12:16:08Zen
dc.date.available2015-07-27T12:16:08Zen
dc.date.issued2015-07-21en
dc.identifier.citationFunctionalized PDMS with versatile and scalable surface roughness gradients for cell culture 2015:150721053642001 ACS Applied Materials & Interfacesen
dc.identifier.issn1944-8244en
dc.identifier.issn1944-8252en
dc.identifier.doi10.1021/acsami.5b04032en
dc.identifier.urihttp://hdl.handle.net/10754/561062en
dc.description.abstractThis manuscript describes a simple and versatile approach to engineering surface roughness gradients via combination of microfluidics and photo-polymerization. Through UV-mediated polymerization, N-isopropylacrylamide with concentration gradients are successfully grafted onto PDMS surface, leading to diverse roughness degrees on the obtained PDMS substrate. Furthermore, the extent of surface roughness can be controllably regulated via tuning the flow rate ratio between the monomer solution and deionized water. Average roughness ranging from 8.050 nm to 151.68 nm has well been achieved in this work. Such PDMS samples are also demonstrated to be capable of working as supporting substrates for controlling cell adhesion or detachment. Due to the different degrees of surface roughness on a single substrate, our method provides an effective approach for designing advanced surafecs for cell culture. Finally, the thermosensitive property of N-isopropylacrylamide makes our sample furnish as another means for controlling the cell detachment from the substrates with correspondence to the surrounding temperature.en
dc.relation.urlhttp://pubs.acs.org/doi/abs/10.1021/acsami.5b04032en
dc.titleFunctionalized PDMS with versatile and scalable surface roughness gradients for cell cultureen
dc.typeArticleen
dc.identifier.journalACS Applied Materials & Interfacesen
dc.contributor.institutionNano Science and Technology Program, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kongen
dc.contributor.institutionBiomedical Research Institute, Shenzhen Peking University - The Hong Kong University of Science and Technology Medical Center, Shenzhen, People’s Republic of Chinaen
dc.contributor.institutionDepartment of Physics, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kongen
dc.contributor.institutionEnvironmental Science Programs, School of Science, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kongen
dc.contributor.institutionThe Soft Matter and Interdisciplinary Research Inst itute, College of Physics, Chongqing University, Chongqing, Chinaen
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