The Arabidopsis RNA-Binding Protein AtRGGA Regulates Tolerance to Salt and Drought Stress

Handle URI:
http://hdl.handle.net/10754/560580
Title:
The Arabidopsis RNA-Binding Protein AtRGGA Regulates Tolerance to Salt and Drought Stress
Authors:
Ambrosone, Alfredo; Batelli, Giorgia; Nurcato, Roberta; Aurilia, Vincenzo; Punzo, Paola; Bangarusamy, Dhinoth Kumar; Ruberti, Ida; Sassi, Massimiliano; Leone, Antonietta; Costa, Antonello; Grillo, Stefania
Abstract:
Salt and drought stress severely reduce plant growth and crop productivity worldwide. The identification of genes underlying stress response and tolerance is the subject of intense research in plant biology. Through microarray analyses, we previously identified in potato (Solanum tuberosum) StRGGA, coding for an Arginine Glycine Glycine (RGG) box-containing RNA-binding protein, whose expression was specifically induced in potato cell cultures gradually exposed to osmotic stress. Here, we show that the Arabidopsis (Arabidopsis thaliana) ortholog, AtRGGA, is a functional RNA-binding protein required for a proper response to osmotic stress. AtRGGA gene expression was up-regulated in seedlings after long-term exposure to abscisic acid (ABA) and polyethylene glycol, while treatments with NaCl resulted in AtRGGA down-regulation. AtRGGA promoter analysis showed activity in several tissues, including stomata, the organs controlling transpiration. Fusion of AtRGGA with yellow fluorescent protein indicated that AtRGGA is localized in the cytoplasm and the cytoplasmic perinuclear region. In addition, the rgga knockout mutant was hypersensitive to ABA in root growth and survival tests and to salt stress during germination and at the vegetative stage. AtRGGA-overexpressing plants showed higher tolerance to ABA and salt stress on plates and in soil, accumulating lower levels of proline when exposed to drought stress. Finally, a global analysis of gene expression revealed extensive alterations in the transcriptome under salt stress, including several genes such as ASCORBATE PEROXIDASE2, GLUTATHIONE S-TRANSFERASE TAU9, and several SMALL AUXIN UPREGULATED RNA-like genes showing opposite expression behavior in transgenic and knockout plants. Taken together, our results reveal an important role of AtRGGA in the mechanisms of plant response and adaptation to stress.
KAUST Department:
Biosciences Core Lab
Citation:
The Arabidopsis RNA-Binding Protein AtRGGA Regulates Tolerance to Salt and Drought Stress 2015, 168 (1):292 Plant Physiology
Journal:
Plant Physiology
Issue Date:
17-Mar-2015
DOI:
10.1104/pp.114.255802
PubMed ID:
25783413
Type:
Article
ISSN:
0032-0889; 1532-2548
Additional Links:
http://www.plantphysiol.org/lookup/doi/10.1104/pp.114.255802
Appears in Collections:
Articles; Biosciences Core Lab

Full metadata record

DC FieldValue Language
dc.contributor.authorAmbrosone, Alfredoen
dc.contributor.authorBatelli, Giorgiaen
dc.contributor.authorNurcato, Robertaen
dc.contributor.authorAurilia, Vincenzoen
dc.contributor.authorPunzo, Paolaen
dc.contributor.authorBangarusamy, Dhinoth Kumaren
dc.contributor.authorRuberti, Idaen
dc.contributor.authorSassi, Massimilianoen
dc.contributor.authorLeone, Antoniettaen
dc.contributor.authorCosta, Antonelloen
dc.contributor.authorGrillo, Stefaniaen
dc.date.accessioned2015-07-16T14:09:39Zen
dc.date.available2015-07-16T14:09:39Zen
dc.date.issued2015-03-17en
dc.identifier.citationThe Arabidopsis RNA-Binding Protein AtRGGA Regulates Tolerance to Salt and Drought Stress 2015, 168 (1):292 Plant Physiologyen
dc.identifier.issn0032-0889en
dc.identifier.issn1532-2548en
dc.identifier.pmid25783413en
dc.identifier.doi10.1104/pp.114.255802en
dc.identifier.urihttp://hdl.handle.net/10754/560580en
dc.description.abstractSalt and drought stress severely reduce plant growth and crop productivity worldwide. The identification of genes underlying stress response and tolerance is the subject of intense research in plant biology. Through microarray analyses, we previously identified in potato (Solanum tuberosum) StRGGA, coding for an Arginine Glycine Glycine (RGG) box-containing RNA-binding protein, whose expression was specifically induced in potato cell cultures gradually exposed to osmotic stress. Here, we show that the Arabidopsis (Arabidopsis thaliana) ortholog, AtRGGA, is a functional RNA-binding protein required for a proper response to osmotic stress. AtRGGA gene expression was up-regulated in seedlings after long-term exposure to abscisic acid (ABA) and polyethylene glycol, while treatments with NaCl resulted in AtRGGA down-regulation. AtRGGA promoter analysis showed activity in several tissues, including stomata, the organs controlling transpiration. Fusion of AtRGGA with yellow fluorescent protein indicated that AtRGGA is localized in the cytoplasm and the cytoplasmic perinuclear region. In addition, the rgga knockout mutant was hypersensitive to ABA in root growth and survival tests and to salt stress during germination and at the vegetative stage. AtRGGA-overexpressing plants showed higher tolerance to ABA and salt stress on plates and in soil, accumulating lower levels of proline when exposed to drought stress. Finally, a global analysis of gene expression revealed extensive alterations in the transcriptome under salt stress, including several genes such as ASCORBATE PEROXIDASE2, GLUTATHIONE S-TRANSFERASE TAU9, and several SMALL AUXIN UPREGULATED RNA-like genes showing opposite expression behavior in transgenic and knockout plants. Taken together, our results reveal an important role of AtRGGA in the mechanisms of plant response and adaptation to stress.en
dc.relation.urlhttp://www.plantphysiol.org/lookup/doi/10.1104/pp.114.255802en
dc.rightsArchived with thanks to Plant Physiologyen
dc.titleThe Arabidopsis RNA-Binding Protein AtRGGA Regulates Tolerance to Salt and Drought Stressen
dc.typeArticleen
dc.contributor.departmentBiosciences Core Laben
dc.identifier.journalPlant Physiologyen
dc.eprint.versionPost-printen
dc.contributor.institutionNational Research Council of Italy, Institute of Biosciences and Bioresources, Research Division Portici, 80055 Portici (Naples), Italy (A.A., G.B., R.N., P.P., A.C., S.G.)en
dc.contributor.institutionNational Research Council of Italy, Institute for Mediterranean Agriculture and Forest Systems, 80056 Ercolano (Naples), Italy (V.A.)en
dc.contributor.institutionNational Research Council of Italy, Institute of Molecular Biology and Pathology, 00185 Rome, Italy (I.R., M.S.)en
dc.contributor.institutionDepartment of Pharmacy, University of Salerno, 84084 Fisciano (Salerno), Italy (A.L.)en
kaust.authorBangarusamy, Dhinoth Kumaren
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