Assembly of photosynthetic reaction center with ABA tri-block polymersomes: highlights on the protein localization.

Handle URI:
http://hdl.handle.net/10754/559597
Title:
Assembly of photosynthetic reaction center with ABA tri-block polymersomes: highlights on the protein localization.
Authors:
Tangorra, Roberto Rocco; Operamolla, Alessandra; Milano, Francesco; Hassan Omar, Omar; Henrard, John; Comparelli, Roberto; Italiano, Francesca; Agostiano, Angela; De Leo, Vincenzo; Marotta, Roberto; Falqui, Andrea ( 0000-0002-1476-7742 ) ; Farinola, Gianluca; Trotta, Massimo
Abstract:
The reconstitution of the integral membrane protein photosynthetic reaction center (RC) in polymersomes, i. e. artificial closed vesicles, was achieved by the micelle-to-vesicle transition technique, a very mild protocol based on size exclusion chromatography often used to drive the incorporation of proteins contemporarily to liposomes formation. An optimized protocol was used to successfully reconstitute the protein in a fully active state in polymersomes formed by the tri-block copolymers PMOXA22-PDMS61-PMOXA22. The RC is very sensitive to its solubilizing environment and was used to probe the positioning of the protein in the vesicles. According to charge-recombination experiments and to the enzymatic activity assay, the RC is found to accommodate in the PMOXA22 region of the polymersome, facing the water bulk solution, rather than in the PDMS61 transmembrane-like region. Furthermore, polymersomes were found to preserve protein integrity efficiently as the biomimetic lipid bilayers but show a much longer temporal stability than lipid based vesicles.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division
Citation:
Assembly of photosynthetic reaction center with ABA tri-block polymersomes: highlights on the protein localization. 2015 Photochem. Photobiol. Sci.
Journal:
Photochem. Photobiol. Sci.
Issue Date:
7-Jul-2015
DOI:
10.1039/C5PP00189G
Type:
Article
ISSN:
1474-905X; 1474-9092
Additional Links:
http://pubs.rsc.org/en/Content/ArticleLanding/2015/PP/C5PP00189G
Appears in Collections:
Articles; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorTangorra, Roberto Roccoen
dc.contributor.authorOperamolla, Alessandraen
dc.contributor.authorMilano, Francescoen
dc.contributor.authorHassan Omar, Omaren
dc.contributor.authorHenrard, Johnen
dc.contributor.authorComparelli, Robertoen
dc.contributor.authorItaliano, Francescaen
dc.contributor.authorAgostiano, Angelaen
dc.contributor.authorDe Leo, Vincenzoen
dc.contributor.authorMarotta, Robertoen
dc.contributor.authorFalqui, Andreaen
dc.contributor.authorFarinola, Gianlucaen
dc.contributor.authorTrotta, Massimoen
dc.date.accessioned2015-07-12T09:07:29Zen
dc.date.available2015-07-12T09:07:29Zen
dc.date.issued2015-07-07en
dc.identifier.citationAssembly of photosynthetic reaction center with ABA tri-block polymersomes: highlights on the protein localization. 2015 Photochem. Photobiol. Sci.en
dc.identifier.issn1474-905Xen
dc.identifier.issn1474-9092en
dc.identifier.doi10.1039/C5PP00189Gen
dc.identifier.urihttp://hdl.handle.net/10754/559597en
dc.description.abstractThe reconstitution of the integral membrane protein photosynthetic reaction center (RC) in polymersomes, i. e. artificial closed vesicles, was achieved by the micelle-to-vesicle transition technique, a very mild protocol based on size exclusion chromatography often used to drive the incorporation of proteins contemporarily to liposomes formation. An optimized protocol was used to successfully reconstitute the protein in a fully active state in polymersomes formed by the tri-block copolymers PMOXA22-PDMS61-PMOXA22. The RC is very sensitive to its solubilizing environment and was used to probe the positioning of the protein in the vesicles. According to charge-recombination experiments and to the enzymatic activity assay, the RC is found to accommodate in the PMOXA22 region of the polymersome, facing the water bulk solution, rather than in the PDMS61 transmembrane-like region. Furthermore, polymersomes were found to preserve protein integrity efficiently as the biomimetic lipid bilayers but show a much longer temporal stability than lipid based vesicles.en
dc.relation.urlhttp://pubs.rsc.org/en/Content/ArticleLanding/2015/PP/C5PP00189Gen
dc.rightsArchived with thanks to Photochem. Photobiol. Sci.en
dc.titleAssembly of photosynthetic reaction center with ABA tri-block polymersomes: highlights on the protein localization.en
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalPhotochem. Photobiol. Sci.en
dc.eprint.versionPost-printen
dc.contributor.institutionChemistry Department, Università degli Studi di Bari “A ldo Moro”, via Orabona 4, 70125 Bari 7 Italyen
dc.contributor.institutionCNR7IPCF, Istituto per i processi Chimico7Fisici, UOS Bari (Italy). via Orabona 4, 70125 Bari – Italyen
dc.contributor.institutionCNR7ICCOM, Istituto di Chimica dei Composti Organomet allici, via Orabona 4, 70125 Bari – Italyen
dc.contributor.institutionChemistry Department and Namur Research College (NARC), University of Namur (UNamur), Rue de Bruxelles 61, Namur , Belgiumen
dc.contributor.institutionElectron Microscopy Lab, Nanochemistry Department Istitut o Italiano di Tecnologia (IIT) Via Morego 30, 16163 Genova – Italyen
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