Pyrosequencing revealed shifts of prokaryotic communities between healthy and disease-like tissues of the Red Sea sponge Crella cyathophora

Handle URI:
http://hdl.handle.net/10754/557018
Title:
Pyrosequencing revealed shifts of prokaryotic communities between healthy and disease-like tissues of the Red Sea sponge Crella cyathophora
Authors:
Gao, Zhao-Ming; Wang, Yong; Tian, Ren-Mao; Lee, On On; Wong, Yue Him; Batang, Zenon B. ( 0000-0003-4593-0589 ) ; Al-Suwailem, Abdulaziz M.; Lafi, Feras Fawzi; Bajic, Vladimir B. ( 0000-0001-5435-4750 ) ; Qian, Pei-Yuan
Abstract:
Sponge diseases have been widely reported, yet the causal factors and major pathogenic microbes remain elusive. In this study, two individuals of the sponge Crella cyathophora in total that showed similar disease-like characteristics were collected from two different locations along the Red Sea coast separated by more than 30 kilometers. The disease-like parts of the two individuals were both covered by green surfaces, and the body size was much smaller compared with adjacent healthy regions. Here, using high-throughput pyrosequencing technology, we investigated the prokaryotic communities in healthy and disease-like sponge tissues as well as adjacent seawater. Microbes in healthy tissues belonged mainly to the Proteobacteria, Cyanobacteria and Bacteroidetes, and were much more diverse at the phylum level than reported previously. Interestingly, the disease-like tissues from the two sponge individuals underwent shifts of prokaryotic communities and were both enriched with a novel clade affiliated with the phylum Verrucomicrobia, implying its intimate connection with the disease-like Red Sea sponge C. cyathophora. Enrichment of the phylum Verrucomicrobia was also considered to be correlated with the presence of algae assemblages forming the green surface of the disease-like sponge tissues. This finding represents an interesting case of sponge disease and is valuable for further study.
KAUST Department:
Coastal and Marine Resources Core Lab; Computational Bioscience Research Center (CBRC)
Citation:
Pyrosequencing revealed shifts of prokaryotic communities between healthy and disease-like tissues of the Red Sea sponge Crella cyathophora 2015, 3:e890 PeerJ
Publisher:
PeerJ
Journal:
PeerJ
Issue Date:
11-Jun-2015
DOI:
10.7717/peerj.890
Type:
Article
ISSN:
2167-8359
Additional Links:
https://peerj.com/articles/890
Appears in Collections:
Articles; Coastal and Marine Resources Core Lab; Computational Bioscience Research Center (CBRC)

Full metadata record

DC FieldValue Language
dc.contributor.authorGao, Zhao-Mingen
dc.contributor.authorWang, Yongen
dc.contributor.authorTian, Ren-Maoen
dc.contributor.authorLee, On Onen
dc.contributor.authorWong, Yue Himen
dc.contributor.authorBatang, Zenon B.en
dc.contributor.authorAl-Suwailem, Abdulaziz M.en
dc.contributor.authorLafi, Feras Fawzien
dc.contributor.authorBajic, Vladimir B.en
dc.contributor.authorQian, Pei-Yuanen
dc.date.accessioned2015-06-17T13:49:18Zen
dc.date.available2015-06-17T13:49:18Zen
dc.date.issued2015-06-11en
dc.identifier.citationPyrosequencing revealed shifts of prokaryotic communities between healthy and disease-like tissues of the Red Sea sponge Crella cyathophora 2015, 3:e890 PeerJen
dc.identifier.issn2167-8359en
dc.identifier.doi10.7717/peerj.890en
dc.identifier.urihttp://hdl.handle.net/10754/557018en
dc.description.abstractSponge diseases have been widely reported, yet the causal factors and major pathogenic microbes remain elusive. In this study, two individuals of the sponge Crella cyathophora in total that showed similar disease-like characteristics were collected from two different locations along the Red Sea coast separated by more than 30 kilometers. The disease-like parts of the two individuals were both covered by green surfaces, and the body size was much smaller compared with adjacent healthy regions. Here, using high-throughput pyrosequencing technology, we investigated the prokaryotic communities in healthy and disease-like sponge tissues as well as adjacent seawater. Microbes in healthy tissues belonged mainly to the Proteobacteria, Cyanobacteria and Bacteroidetes, and were much more diverse at the phylum level than reported previously. Interestingly, the disease-like tissues from the two sponge individuals underwent shifts of prokaryotic communities and were both enriched with a novel clade affiliated with the phylum Verrucomicrobia, implying its intimate connection with the disease-like Red Sea sponge C. cyathophora. Enrichment of the phylum Verrucomicrobia was also considered to be correlated with the presence of algae assemblages forming the green surface of the disease-like sponge tissues. This finding represents an interesting case of sponge disease and is valuable for further study.en
dc.publisherPeerJen
dc.relation.urlhttps://peerj.com/articles/890en
dc.rightsThis is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.http://creativecommons.org/licenses/by/4.0/en
dc.subjectLow microbial abundanceen
dc.subjectVerrucomicrobiaen
dc.subjectSponge symbionten
dc.subjectDisease-like spongeen
dc.titlePyrosequencing revealed shifts of prokaryotic communities between healthy and disease-like tissues of the Red Sea sponge Crella cyathophoraen
dc.typeArticleen
dc.contributor.departmentCoastal and Marine Resources Core Laben
dc.contributor.departmentComputational Bioscience Research Center (CBRC)en
dc.identifier.journalPeerJen
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionDivision of Life Science, The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong, PR Chinaen
dc.contributor.institutionSanya Institute of Deep Sea Science and Engineering, Chinese Academy of Sciences, Sanya, Hai Nan, PR Chinaen
kaust.authorBajic, Vladimir B.en
kaust.authorBatang, Zenon B.en
kaust.authorAl-Suwailem, Abdulaziz M.en
kaust.authorLafi, Feras Fawzien
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