Compositional Similarities and Differences between Transparent Exopolymer Particles (TEP) from two Marine Bacteria and two Marine Algae: Significance to Surface Biofouling

Handle URI:
http://hdl.handle.net/10754/557006
Title:
Compositional Similarities and Differences between Transparent Exopolymer Particles (TEP) from two Marine Bacteria and two Marine Algae: Significance to Surface Biofouling
Authors:
Li, Sheng ( 0000-0002-9921-4951 ) ; Winters, H.; Villacorte, L.O.; Ekowati, Y.; Emwas, Abdul-Hamid.; Kennedy, M.D.; Amy, Gary L.
Abstract:
Transparent-exopolymer-particles (TEP) have been recently identified as a significant contributor to surface biofouling, such as on reverse osmosis (RO) membranes. TEP research has mainly focused on algal TEP/TEP precursors while limited investigations have been conducted on those released by bacteria. In this study, TEP/TEP precursors derived from both algae and bacteria were isolated and then characterized to investigate their similarities and/or differences using various advanced analytical techniques, thus providing a better understanding of their potential effect on biofouling. Bacterial TEP/TEP precursors were isolated from two species of marine bacteria (Pseudidiomarina homiensis and Pseudoalteromonas atlantica) while algal TEP/TEP precursors were isolated from two marine algae species (Alexandrium tamarense and Chaetoceros affinis). Results indicated that both isolated bacterial and algal TEP/TEP precursors were associated with protein-like materials, and most TEP precursors were high-molecular-weight biopolymers. Furthermore all investigated algal and bacterial TEP/TEP precursors showed a lectin-like property, which can enable them to act as a chemical conditioning layer and to agglutinate bacteria. This property may enhance surface biofouling. However, both proton nuclear magnetic resonance (NMR) spectra and the nitrogen/carbon (N/C) ratios suggested that the algal TEP/TEP precursors contained much less protein content than the bacterial TEP/TEP precursors. This difference may influence their initial deposition and further development of surface biofouling.
KAUST Department:
Water Desalination and Reuse Research Center (WDRC); Nuclear Magnetic Resonance Core Lab
Citation:
Compositional Similarities and Differences between Transparent Exopolymer Particles (TEP) from two Marine Bacteria and two Marine Algae: Significance to Surface Biofouling 2015 Marine Chemistry
Publisher:
Elsevier BV
Journal:
Marine Chemistry
Issue Date:
12-Jun-2015
DOI:
10.1016/j.marchem.2015.06.009
Type:
Article
ISSN:
03044203
Additional Links:
http://linkinghub.elsevier.com/retrieve/pii/S0304420315001243
Appears in Collections:
Articles; Water Desalination and Reuse Research Center (WDRC)

Full metadata record

DC FieldValue Language
dc.contributor.authorLi, Shengen
dc.contributor.authorWinters, H.en
dc.contributor.authorVillacorte, L.O.en
dc.contributor.authorEkowati, Y.en
dc.contributor.authorEmwas, Abdul-Hamid.en
dc.contributor.authorKennedy, M.D.en
dc.contributor.authorAmy, Gary L.en
dc.date.accessioned2015-06-17T13:41:04Zen
dc.date.available2015-06-17T13:41:04Zen
dc.date.issued2015-06-12en
dc.identifier.citationCompositional Similarities and Differences between Transparent Exopolymer Particles (TEP) from two Marine Bacteria and two Marine Algae: Significance to Surface Biofouling 2015 Marine Chemistryen
dc.identifier.issn03044203en
dc.identifier.doi10.1016/j.marchem.2015.06.009en
dc.identifier.urihttp://hdl.handle.net/10754/557006en
dc.description.abstractTransparent-exopolymer-particles (TEP) have been recently identified as a significant contributor to surface biofouling, such as on reverse osmosis (RO) membranes. TEP research has mainly focused on algal TEP/TEP precursors while limited investigations have been conducted on those released by bacteria. In this study, TEP/TEP precursors derived from both algae and bacteria were isolated and then characterized to investigate their similarities and/or differences using various advanced analytical techniques, thus providing a better understanding of their potential effect on biofouling. Bacterial TEP/TEP precursors were isolated from two species of marine bacteria (Pseudidiomarina homiensis and Pseudoalteromonas atlantica) while algal TEP/TEP precursors were isolated from two marine algae species (Alexandrium tamarense and Chaetoceros affinis). Results indicated that both isolated bacterial and algal TEP/TEP precursors were associated with protein-like materials, and most TEP precursors were high-molecular-weight biopolymers. Furthermore all investigated algal and bacterial TEP/TEP precursors showed a lectin-like property, which can enable them to act as a chemical conditioning layer and to agglutinate bacteria. This property may enhance surface biofouling. However, both proton nuclear magnetic resonance (NMR) spectra and the nitrogen/carbon (N/C) ratios suggested that the algal TEP/TEP precursors contained much less protein content than the bacterial TEP/TEP precursors. This difference may influence their initial deposition and further development of surface biofouling.en
dc.publisherElsevier BVen
dc.relation.urlhttp://linkinghub.elsevier.com/retrieve/pii/S0304420315001243en
dc.rightsNOTICE: this is the author’s version of a work that was accepted for publication in Marine Chemistry. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Marine Chemistry, 12 June 2015 DOI: 10.1016/j.marchem.2015.06.009en
dc.subjecttransparent exopolymer particlesen
dc.subjectreverse osmosisen
dc.subjectbiofoulingen
dc.subjectmarineen
dc.subjectbacteriaen
dc.subjectalgaeen
dc.titleCompositional Similarities and Differences between Transparent Exopolymer Particles (TEP) from two Marine Bacteria and two Marine Algae: Significance to Surface Biofoulingen
dc.typeArticleen
dc.contributor.departmentWater Desalination and Reuse Research Center (WDRC)en
dc.contributor.departmentNuclear Magnetic Resonance Core Laben
dc.identifier.journalMarine Chemistryen
dc.eprint.versionPost-printen
dc.contributor.institutionUNESCO-IHE, Institute for Water Education, Westvest 7, 2611 AX, Delft, The Netherlandsen
dc.contributor.institutionWetsus Center of Excellence for Sustainable Water Technology, Agora 1, 8934 CJ Leeuwarden, Netherlandsen
dc.contributor.institutionFairleigh Dickinson University, Teaneck, NJ 07666, USAen
kaust.authorEmwas, Abdul-Hamid M.en
kaust.authorAmy, Gary L.en
kaust.authorLi, Shengen
kaust.authorWinters, H.en
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