The draft genome of Mycobacterium aurum, a potential model organism for investigating drugs against Mycobacterium tuberculosis and Mycobacterium leprae

Handle URI:
http://hdl.handle.net/10754/556900
Title:
The draft genome of Mycobacterium aurum, a potential model organism for investigating drugs against Mycobacterium tuberculosis and Mycobacterium leprae
Authors:
Phelan, Jody; Maitra, Arundhati; McNerney, Ruth; Nair, Mridul; Gupta, Antima; Coll, Francesc; Pain, Arnab ( 0000-0002-1755-2819 ) ; Bhakta, Sanjib; Clark, Taane G.
Abstract:
Mycobacterium aurum (M. aurum) is an environmental mycobacteria that has previously been used in studies of anti-mycobacterial drugs due to its fast growth rate and low pathogenicity. The M. aurum genome has been sequenced and assembled into 46 contigs, with a total length of 6.02 Mb containing 5684 annotated protein-coding genes. A phylogenetic analysis using whole genome alignments positioned M. aurum close to Mycobacterium vaccae and Mycobacterium vanbaalenii, within a clade related to fast-growing mycobacteria. Large-scale genomic rearrangements were identified by comparing the M. aurum genome to those of Mycobacterium tuberculosis and Mycobacterium leprae. M. aurum orthologous genes implicated in resistance to anti-tuberculosis drugs in M. tuberculosis were observed. The sequence identity at the DNA level varied from 68.6% for pncA (pyrazinamide drug-related) to 96.2% for rrs (streptomycin, capreomycin). We observed two homologous genes encoding the catalase-peroxidase enzyme (katG) that is associated with resistance to isoniazid. Similarly, two embB homologues were identified in the M. aurum genome. In addition to describing for the first time the genome of M. aurum, this work provides a resource to aid the use of M. aurum in studies to develop improved drugs for the pathogenic mycobacteria M. tuberculosis and M. leprae.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division
Citation:
The draft genome of Mycobacterium aurum, a potential model organism for investigating drugs against Mycobacterium tuberculosis and Mycobacterium leprae 2015 International Journal of Mycobacteriology
Publisher:
Elsevier BV
Journal:
International Journal of Mycobacteriology
Issue Date:
4-Jun-2015
DOI:
10.1016/j.ijmyco.2015.05.001
Type:
Article
ISSN:
22125531
Additional Links:
http://linkinghub.elsevier.com/retrieve/pii/S2212553115000783
Appears in Collections:
Articles; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorPhelan, Jodyen
dc.contributor.authorMaitra, Arundhatien
dc.contributor.authorMcNerney, Ruthen
dc.contributor.authorNair, Mridulen
dc.contributor.authorGupta, Antimaen
dc.contributor.authorColl, Francescen
dc.contributor.authorPain, Arnaben
dc.contributor.authorBhakta, Sanjiben
dc.contributor.authorClark, Taane G.en
dc.date.accessioned2015-06-14T13:16:22Zen
dc.date.available2015-06-14T13:16:22Zen
dc.date.issued2015-06-04en
dc.identifier.citationThe draft genome of Mycobacterium aurum, a potential model organism for investigating drugs against Mycobacterium tuberculosis and Mycobacterium leprae 2015 International Journal of Mycobacteriologyen
dc.identifier.issn22125531en
dc.identifier.doi10.1016/j.ijmyco.2015.05.001en
dc.identifier.urihttp://hdl.handle.net/10754/556900en
dc.description.abstractMycobacterium aurum (M. aurum) is an environmental mycobacteria that has previously been used in studies of anti-mycobacterial drugs due to its fast growth rate and low pathogenicity. The M. aurum genome has been sequenced and assembled into 46 contigs, with a total length of 6.02 Mb containing 5684 annotated protein-coding genes. A phylogenetic analysis using whole genome alignments positioned M. aurum close to Mycobacterium vaccae and Mycobacterium vanbaalenii, within a clade related to fast-growing mycobacteria. Large-scale genomic rearrangements were identified by comparing the M. aurum genome to those of Mycobacterium tuberculosis and Mycobacterium leprae. M. aurum orthologous genes implicated in resistance to anti-tuberculosis drugs in M. tuberculosis were observed. The sequence identity at the DNA level varied from 68.6% for pncA (pyrazinamide drug-related) to 96.2% for rrs (streptomycin, capreomycin). We observed two homologous genes encoding the catalase-peroxidase enzyme (katG) that is associated with resistance to isoniazid. Similarly, two embB homologues were identified in the M. aurum genome. In addition to describing for the first time the genome of M. aurum, this work provides a resource to aid the use of M. aurum in studies to develop improved drugs for the pathogenic mycobacteria M. tuberculosis and M. leprae.en
dc.publisherElsevier BVen
dc.relation.urlhttp://linkinghub.elsevier.com/retrieve/pii/S2212553115000783en
dc.rightsArchived with thanks to International Journal of Mycobacteriology, Under a Creative Commons license, http://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.subjectGenomeen
dc.subjectDrug screeningen
dc.subjectM. lepraeen
dc.subjectM. tuberculosisen
dc.subjectM. aurumen
dc.subjectMycobacteriaen
dc.titleThe draft genome of Mycobacterium aurum, a potential model organism for investigating drugs against Mycobacterium tuberculosis and Mycobacterium lepraeen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalInternational Journal of Mycobacteriologyen
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionFaculty of Infectious and Tropical Diseases, London School of Hygiene & Tropical Medicine, Keppel Street, London WC1E 7HT, United Kingdomen
dc.contributor.institutionMycobacteria Research Laboratory, Institute of Structural and Molecular Biology, Department of Biological Sciences, Birkbeck College, University of London, Malet Street, London WC1E 7HX, United Kingdomen
dc.contributor.institutionFaculty of Epidemiology and Population Health, London School of Hygiene & Tropical Medicine, Keppel Street, London WC1E 7HT, United Kingdomen
kaust.authorNair, Mridulen
kaust.authorPain, Arnaben
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