The Arabidopsis Vacuolar Sorting Receptor1 Is Required for Osmotic Stress-Induced Abscisic Acid Biosynthesis

Handle URI:
http://hdl.handle.net/10754/556897
Title:
The Arabidopsis Vacuolar Sorting Receptor1 Is Required for Osmotic Stress-Induced Abscisic Acid Biosynthesis
Authors:
Wang, Zhen-Yu; Gehring, Christoph A. ( 0000-0003-4355-4591 ) ; Zhu, Jianhua; Li, Feng-Min; Zhu, Jian-Kang ( 0000-0001-5134-731X ) ; Xiong, Liming ( 0000-0001-8099-0806 )
Abstract:
Osmotic stress activates the biosynthesis of the phytohormone abscisic acid (ABA) through a pathway that is rate limited by the carotenoid cleavage enzyme 9-cis-epoxycarotenoid dioxygenase (NCED). To understand the signal transduction mechanism underlying the activation of ABA biosynthesis, we performed a forward genetic screen to isolate mutants defective in osmotic stress regulation of the NCED3 gene. Here, we identified the Arabidopsis (Arabidopsis thaliana) Vacuolar Sorting Receptor1 (VSR1) as a unique regulator of ABA biosynthesis. The vsr1 mutant not only shows increased sensitivity to osmotic stress, but also is defective in the feedback regulation of ABA biosynthesis by ABA. Further analysis revealed that vacuolar trafficking mediated by VSR1 is required for osmotic stress-responsive ABA biosynthesis and osmotic stress tolerance. Moreover, under osmotic stress conditions, the membrane potential, calcium flux, and vacuolar pH changes in the vsr1 mutant differ from those in the wild type. Given that manipulation of the intracellular pH is sufficient to modulate the expression of ABA biosynthesis genes, including NCED3, and ABA accumulation, we propose that intracellular pH changes caused by osmotic stress may play a signaling role in regulating ABA biosynthesis and that this regulation is dependent on functional VSR1.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division
Citation:
The Arabidopsis Vacuolar Sorting Receptor1 Is Required for Osmotic Stress-Induced Abscisic Acid Biosynthesis 2015, 167 (1):137 Plant Physiology
Publisher:
American Society of Plant Biologists (ASPB)
Journal:
Plant Physiology
Issue Date:
21-Nov-2014
DOI:
10.1104/pp.114.249268
PubMed ID:
25416474
PubMed Central ID:
PMC4281004
Type:
Article
ISSN:
0032-0889; 1532-2548
Additional Links:
http://www.plantphysiol.org/lookup/doi/10.1104/pp.114.249268
Appears in Collections:
Articles; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorWang, Zhen-Yuen
dc.contributor.authorGehring, Christoph A.en
dc.contributor.authorZhu, Jianhuaen
dc.contributor.authorLi, Feng-Minen
dc.contributor.authorZhu, Jian-Kangen
dc.contributor.authorXiong, Limingen
dc.date.accessioned2015-06-14T13:13:41Zen
dc.date.available2015-06-14T13:13:41Zen
dc.date.issued2014-11-21en
dc.identifier.citationThe Arabidopsis Vacuolar Sorting Receptor1 Is Required for Osmotic Stress-Induced Abscisic Acid Biosynthesis 2015, 167 (1):137 Plant Physiologyen
dc.identifier.issn0032-0889en
dc.identifier.issn1532-2548en
dc.identifier.pmid25416474en
dc.identifier.doi10.1104/pp.114.249268en
dc.identifier.urihttp://hdl.handle.net/10754/556897en
dc.description.abstractOsmotic stress activates the biosynthesis of the phytohormone abscisic acid (ABA) through a pathway that is rate limited by the carotenoid cleavage enzyme 9-cis-epoxycarotenoid dioxygenase (NCED). To understand the signal transduction mechanism underlying the activation of ABA biosynthesis, we performed a forward genetic screen to isolate mutants defective in osmotic stress regulation of the NCED3 gene. Here, we identified the Arabidopsis (Arabidopsis thaliana) Vacuolar Sorting Receptor1 (VSR1) as a unique regulator of ABA biosynthesis. The vsr1 mutant not only shows increased sensitivity to osmotic stress, but also is defective in the feedback regulation of ABA biosynthesis by ABA. Further analysis revealed that vacuolar trafficking mediated by VSR1 is required for osmotic stress-responsive ABA biosynthesis and osmotic stress tolerance. Moreover, under osmotic stress conditions, the membrane potential, calcium flux, and vacuolar pH changes in the vsr1 mutant differ from those in the wild type. Given that manipulation of the intracellular pH is sufficient to modulate the expression of ABA biosynthesis genes, including NCED3, and ABA accumulation, we propose that intracellular pH changes caused by osmotic stress may play a signaling role in regulating ABA biosynthesis and that this regulation is dependent on functional VSR1.en
dc.publisherAmerican Society of Plant Biologists (ASPB)en
dc.relation.urlhttp://www.plantphysiol.org/lookup/doi/10.1104/pp.114.249268en
dc.rightsArchived with thanks to Plant Physiologyen
dc.titleThe Arabidopsis Vacuolar Sorting Receptor1 Is Required for Osmotic Stress-Induced Abscisic Acid Biosynthesisen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalPlant Physiologyen
dc.identifier.pmcidPMC4281004en
dc.eprint.versionPost-printen
dc.contributor.institutionState Key Laboratory of Grassland Agroecosystem, Institute of Arid Agroecology, School of Life Sciences, Lanzhou University, Lanzhou 730000, Gansu Province, Chinaen
dc.contributor.institutionDepartment of Plant Science and Landscape Architecture, University of Maryland, College Park, Maryland 20742en
dc.contributor.institutionDepartment of Horticulture and Landscape Architecture, Purdue University, West Lafayette, Indiana 47907en
dc.contributor.institutionShanghai Center for Plant Stress Biology, Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, Chinaen
kaust.authorWang, Zhenyuen
kaust.authorGehring, Christoph A.en
kaust.authorXiong, Limingen

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