Heterotrophic bacterial responses to the winter–spring phytoplankton bloom in open waters of the NW Mediterranean

Handle URI:
http://hdl.handle.net/10754/556540
Title:
Heterotrophic bacterial responses to the winter–spring phytoplankton bloom in open waters of the NW Mediterranean
Authors:
Gomes, Ana; Gasol, Josep M.; Estrada, Marta; Franco-Vidal, Leticia; Díaz-Pérez, Laura; Ferrera, Isabel; Moran, Xose Anxelu G. ( 0000-0002-9823-5339 )
Abstract:
The response of planktonic heterotrophic prokaryotes to the NW Mediterranean winter–spring offshore phytoplankton bloom was assessed in 3 cruises conducted in March, April–May and September 2009. Bulk measurements of phytoplankton and bacterioplankton biomass and production were complemented with an insight into bacterial physiological structure by single-cell analysis of nucleic acid content [low (LNA) vs. high (HNA)] and membrane integrity (“Live” vs. “Dead” cells). Bacterial production empirical conversion factors (0.82±0.25 SE kg C mol leucine−1) were almost always well below the theoretical value. Major differences in most microbial variables were found among the 3 periods, which varied from extremely high phytoplankton biomass and production during the bloom in March (>1 g C m−2 d−1 primary production) to typically oligotrophic conditions during September stratification (<200 mg C m−2 d−1). In both these periods bacterial production was ~30 mg C m−2 d−1 while very large bacterial production (mean 228, with some stations exceeding 500 mg C m−2 d−1) but low biomass was observed during the April–May post-bloom phase. The contribution of HNA (30–67%) and “Live” cells (47–97%) were temporally opposite in the study periods, with maxima in March and September, respectively. Different relationships were found between physiological structure and bottom-up variables, with HNA bacteria apparently more responsive to phytoplankton only during the bloom, coinciding with larger average cell sizes of LNA bacteria. Moderate phytoplankton–bacterioplankton coupling of biomass and activity was only observed in the bloom and post-bloom phases, while relationships between both compartments were not significant under stratification. With all data pooled, bacteria were only weakly bottom-up controlled. Our analyses show that the biomass and production of planktonic algae and bacteria followed opposite paths in the transition from bloom to oligotrophic conditions.
KAUST Department:
Red Sea Research Center (RSRC)
Citation:
Heterotrophic bacterial responses to the winter–spring phytoplankton bloom in open waters of the NW Mediterranean 2015, 96:59 Deep Sea Research Part I: Oceanographic Research Papers
Publisher:
Elsevier BV
Journal:
Deep Sea Research Part I: Oceanographic Research Papers
Issue Date:
3-Dec-2014
DOI:
10.1016/j.dsr.2014.11.007
Type:
Article
ISSN:
09670637
Additional Links:
http://linkinghub.elsevier.com/retrieve/pii/S0967063714002118
Appears in Collections:
Articles; Red Sea Research Center (RSRC)

Full metadata record

DC FieldValue Language
dc.contributor.authorGomes, Anaen
dc.contributor.authorGasol, Josep M.en
dc.contributor.authorEstrada, Martaen
dc.contributor.authorFranco-Vidal, Leticiaen
dc.contributor.authorDíaz-Pérez, Lauraen
dc.contributor.authorFerrera, Isabelen
dc.contributor.authorMoran, Xose Anxelu G.en
dc.date.accessioned2015-06-07T22:05:55Zen
dc.date.available2015-06-07T22:05:55Zen
dc.date.issued2014-12-03en
dc.identifier.citationHeterotrophic bacterial responses to the winter–spring phytoplankton bloom in open waters of the NW Mediterranean 2015, 96:59 Deep Sea Research Part I: Oceanographic Research Papersen
dc.identifier.issn09670637en
dc.identifier.doi10.1016/j.dsr.2014.11.007en
dc.identifier.urihttp://hdl.handle.net/10754/556540en
dc.description.abstractThe response of planktonic heterotrophic prokaryotes to the NW Mediterranean winter–spring offshore phytoplankton bloom was assessed in 3 cruises conducted in March, April–May and September 2009. Bulk measurements of phytoplankton and bacterioplankton biomass and production were complemented with an insight into bacterial physiological structure by single-cell analysis of nucleic acid content [low (LNA) vs. high (HNA)] and membrane integrity (“Live” vs. “Dead” cells). Bacterial production empirical conversion factors (0.82±0.25 SE kg C mol leucine−1) were almost always well below the theoretical value. Major differences in most microbial variables were found among the 3 periods, which varied from extremely high phytoplankton biomass and production during the bloom in March (>1 g C m−2 d−1 primary production) to typically oligotrophic conditions during September stratification (<200 mg C m−2 d−1). In both these periods bacterial production was ~30 mg C m−2 d−1 while very large bacterial production (mean 228, with some stations exceeding 500 mg C m−2 d−1) but low biomass was observed during the April–May post-bloom phase. The contribution of HNA (30–67%) and “Live” cells (47–97%) were temporally opposite in the study periods, with maxima in March and September, respectively. Different relationships were found between physiological structure and bottom-up variables, with HNA bacteria apparently more responsive to phytoplankton only during the bloom, coinciding with larger average cell sizes of LNA bacteria. Moderate phytoplankton–bacterioplankton coupling of biomass and activity was only observed in the bloom and post-bloom phases, while relationships between both compartments were not significant under stratification. With all data pooled, bacteria were only weakly bottom-up controlled. Our analyses show that the biomass and production of planktonic algae and bacteria followed opposite paths in the transition from bloom to oligotrophic conditions.en
dc.publisherElsevier BVen
dc.relation.urlhttp://linkinghub.elsevier.com/retrieve/pii/S0967063714002118en
dc.rightsNOTICE: this is the author’s version of a work that was accepted for publication in Deep Sea Research Part I: Oceanographic Research Papers. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Deep Sea Research Part I: Oceanographic Research Papers, 3 December 2014. DOI: 10.1016/j.dsr.2014.11.007.en
dc.subjectBacteriaen
dc.subjectPhytoplanktonen
dc.subjectBacterial productionen
dc.subjectPrimary productionen
dc.subjectPhytoplankton–bacterioplankton couplingen
dc.subjectWinter–spring bloomen
dc.subjectMediterraneanen
dc.titleHeterotrophic bacterial responses to the winter–spring phytoplankton bloom in open waters of the NW Mediterraneanen
dc.typeArticleen
dc.contributor.departmentRed Sea Research Center (RSRC)en
dc.identifier.journalDeep Sea Research Part I: Oceanographic Research Papersen
dc.eprint.versionPost-printen
dc.contributor.institutionInstitut de Ciències del Mar, CSIC, Pg. Marítim de la Barceloneta 37-49, 08003 Barcelona, Catalunya, Spainen
dc.contributor.institutionCentro Oceanográfico de Xixón, Instituto Español de Oceanografia, Camín de L’Arbeyal, s/n, 33212 Xixón, Asturies, Spainen
kaust.authorMoran, Xose Anxelu G.en
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