GeoChip-based insights into the microbial functional gene repertoire of marine sponges (high microbial abundance, low microbial abundance) and seawater

Handle URI:
http://hdl.handle.net/10754/556514
Title:
GeoChip-based insights into the microbial functional gene repertoire of marine sponges (high microbial abundance, low microbial abundance) and seawater
Authors:
Bayer, Kristina; Moitinho-Silva, Lucas; Brümmer, Franz; Cannistraci, Carlo V.; Ravasi, Timothy ( 0000-0002-9950-465X ) ; Hentschel, Ute
Abstract:
The GeoChip 4.2 gene array was employed to interrogate the microbial functional gene repertoire of sponges and seawater collected from the Red Sea and the Mediterranean. Complementary amplicon sequencing confirmed the microbial community composition characteristic of high microbial abundance (HMA) and low microbial abundance (LMA) sponges. By use of GeoChip, altogether 20 273 probes encoding for 627 functional genes and representing 16 gene categories were identified. Minimum curvilinear embedding analyses revealed a clear separation between the samples. The HMA/LMA dichotomy was stronger than any possible geographic pattern, which is shown here for the first time on the level of functional genes. However, upon inspection of individual genes, very few specific differences were discernible. Differences were related to microbial ammonia oxidation, ammonification, and archaeal autotrophic carbon fixation (higher gene abundance in sponges over seawater) as well as denitrification and radiation-stress-related genes (lower gene abundance in sponges over seawater). Except for few documented specific differences the functional gene repertoire between the different sources appeared largely similar. This study expands previous reports in that functional gene convergence is not only reported between HMA and LMA sponges but also between sponges and seawater.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division
Citation:
GeoChip-based insights into the microbial functional gene repertoire of marine sponges (high microbial abundance, low microbial abundance) and seawater 2014, 90 (3):832 FEMS Microbiology Ecology
Publisher:
Oxford University Press (OUP)
Journal:
FEMS Microbiology Ecology
Issue Date:
8-Jan-2015
DOI:
10.1111/1574-6941.12441
Type:
Article
ISSN:
01686496
Additional Links:
http://femsec.oxfordjournals.org/cgi/doi/10.1111/1574-6941.12441
Appears in Collections:
Articles; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorBayer, Kristinaen
dc.contributor.authorMoitinho-Silva, Lucasen
dc.contributor.authorBrümmer, Franzen
dc.contributor.authorCannistraci, Carlo V.en
dc.contributor.authorRavasi, Timothyen
dc.contributor.authorHentschel, Uteen
dc.date.accessioned2015-06-07T21:27:17Zen
dc.date.available2015-06-07T21:27:17Zen
dc.date.issued2015-01-08en
dc.identifier.citationGeoChip-based insights into the microbial functional gene repertoire of marine sponges (high microbial abundance, low microbial abundance) and seawater 2014, 90 (3):832 FEMS Microbiology Ecologyen
dc.identifier.issn01686496en
dc.identifier.doi10.1111/1574-6941.12441en
dc.identifier.urihttp://hdl.handle.net/10754/556514en
dc.description.abstractThe GeoChip 4.2 gene array was employed to interrogate the microbial functional gene repertoire of sponges and seawater collected from the Red Sea and the Mediterranean. Complementary amplicon sequencing confirmed the microbial community composition characteristic of high microbial abundance (HMA) and low microbial abundance (LMA) sponges. By use of GeoChip, altogether 20 273 probes encoding for 627 functional genes and representing 16 gene categories were identified. Minimum curvilinear embedding analyses revealed a clear separation between the samples. The HMA/LMA dichotomy was stronger than any possible geographic pattern, which is shown here for the first time on the level of functional genes. However, upon inspection of individual genes, very few specific differences were discernible. Differences were related to microbial ammonia oxidation, ammonification, and archaeal autotrophic carbon fixation (higher gene abundance in sponges over seawater) as well as denitrification and radiation-stress-related genes (lower gene abundance in sponges over seawater). Except for few documented specific differences the functional gene repertoire between the different sources appeared largely similar. This study expands previous reports in that functional gene convergence is not only reported between HMA and LMA sponges but also between sponges and seawater.en
dc.publisherOxford University Press (OUP)en
dc.relation.urlhttp://femsec.oxfordjournals.org/cgi/doi/10.1111/1574-6941.12441en
dc.rightsArchived with thanks to FEMS Microbiology Ecologyen
dc.subjectGeoChipen
dc.subjectamplicon sequencingen
dc.subjectmarine spongeen
dc.subjectPoriferaen
dc.subjectfunctional geneen
dc.subjectsymbiosisen
dc.titleGeoChip-based insights into the microbial functional gene repertoire of marine sponges (high microbial abundance, low microbial abundance) and seawateren
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalFEMS Microbiology Ecologyen
dc.eprint.versionPost-printen
dc.contributor.institutionDepartment of Botany II; Julius-von-Sachs Institute for Biological Sciences; University of Wuerzburg; Wuerzburg Germanyen
dc.contributor.institutionDepartment of Botany II; Julius-von-Sachs Institute for Biological Sciences; University of Wuerzburg; Wuerzburg Germanyen
dc.contributor.institutionDepartment of Zoology; Biological Institute; University of Stuttgart; Stuttgart Germanyen
dc.contributor.institutionDepartment of Botany II; Julius-von-Sachs Institute for Biological Sciences; University of Wuerzburg; Wuerzburg Germanyen
dc.contributor.institutionBiomedical Cybernetics Group, Biotechnology Center (BIOTEC), Technical University Dresden, Dresden, Germanyen
kaust.authorCannistraci, Carloen
kaust.authorRavasi, Timothyen
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