The Na+ transporter, TaHKT1;5-D, limits shoot Na+ accumulation in bread wheat

Handle URI:
http://hdl.handle.net/10754/556197
Title:
The Na+ transporter, TaHKT1;5-D, limits shoot Na+ accumulation in bread wheat
Authors:
Byrt, Caitlin Siobhan; Xu, Bo; Krishnan, Mahima; Lightfoot, Damien ( 0000-0003-3824-8856 ) ; Athman, Asmini; Jacobs, Andrew Keith; Watson-Haigh, Nathan S.; Plett, Darren; Munns, Rana; Tester, Mark A. ( 0000-0002-5085-8801 ) ; Gilliham, Matthew
Abstract:
Bread wheat (Triticum aestivum L.) has a major salt tolerance locus, Kna1, responsible for the maintenance of a high cytosolic K+/Na+ ratio in the leaves of salt stressed plants. The Kna1 locus encompasses a large DNA fragment, the distal 14% of chromosome 4DL. Limited recombination has been observed at this locus making it difficult to map genetically and identify the causal gene. Here, we decipher the function of TaHKT1;5-D, a candidate gene underlying the Kna1 locus. Transport studies using the heterologous expression systems Saccharomyces cerevisiae and Xenopus laevis oocytes indicated that TaHKT1;5-D is a Na+-selective transporter. Transient expression in Arabidopsis thaliana mesophyll protoplasts and in situ polymerase chain reaction indicated that TaHKT1;5-D is localised on the plasma membrane in the wheat root stele. RNA interference-induced silencing decreased the expression of TaHKT1;5-D in transgenic bread wheat lines which led to an increase in the Na+ concentration in the leaves. This indicates that TaHKT1;5-D retrieves Na+ from the xylem vessels in the root and has an important role in restricting the transport of Na+ from the root to the leaves in bread wheat. Thus, TaHKT1;5-D confers the essential salinity tolerance mechanism in bread wheat associated with the Kna1 locus via shoot Na+ exclusion and is critical in maintaining a high K+/Na+ ratio in the leaves. These findings show there is potential to increase the salinity tolerance of bread wheat by manipulation of HKT1;5 genes.
KAUST Department:
Center for Desert Agriculture; Biological and Environmental Sciences and Engineering (BESE) Division
Citation:
The Na+ transporter, TaHKT1;5-D, limits shoot Na+ accumulation in bread wheat 2014, 80 (3):516 The Plant Journal
Publisher:
Wiley-Blackwell
Journal:
The Plant Journal
Issue Date:
1-Oct-2014
DOI:
10.1111/tpj.12651
Type:
Article
ISSN:
09607412
Additional Links:
http://doi.wiley.com/10.1111/tpj.12651
Appears in Collections:
Articles; Center for Desert Agriculture; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorByrt, Caitlin Siobhanen
dc.contributor.authorXu, Boen
dc.contributor.authorKrishnan, Mahimaen
dc.contributor.authorLightfoot, Damienen
dc.contributor.authorAthman, Asminien
dc.contributor.authorJacobs, Andrew Keithen
dc.contributor.authorWatson-Haigh, Nathan S.en
dc.contributor.authorPlett, Darrenen
dc.contributor.authorMunns, Ranaen
dc.contributor.authorTester, Mark A.en
dc.contributor.authorGilliham, Matthewen
dc.date.accessioned2015-06-03T08:38:14Zen
dc.date.available2015-06-03T08:38:14Zen
dc.date.issued2014-10-01en
dc.identifier.citationThe Na+ transporter, TaHKT1;5-D, limits shoot Na+ accumulation in bread wheat 2014, 80 (3):516 The Plant Journalen
dc.identifier.issn09607412en
dc.identifier.doi10.1111/tpj.12651en
dc.identifier.urihttp://hdl.handle.net/10754/556197en
dc.description.abstractBread wheat (Triticum aestivum L.) has a major salt tolerance locus, Kna1, responsible for the maintenance of a high cytosolic K+/Na+ ratio in the leaves of salt stressed plants. The Kna1 locus encompasses a large DNA fragment, the distal 14% of chromosome 4DL. Limited recombination has been observed at this locus making it difficult to map genetically and identify the causal gene. Here, we decipher the function of TaHKT1;5-D, a candidate gene underlying the Kna1 locus. Transport studies using the heterologous expression systems Saccharomyces cerevisiae and Xenopus laevis oocytes indicated that TaHKT1;5-D is a Na+-selective transporter. Transient expression in Arabidopsis thaliana mesophyll protoplasts and in situ polymerase chain reaction indicated that TaHKT1;5-D is localised on the plasma membrane in the wheat root stele. RNA interference-induced silencing decreased the expression of TaHKT1;5-D in transgenic bread wheat lines which led to an increase in the Na+ concentration in the leaves. This indicates that TaHKT1;5-D retrieves Na+ from the xylem vessels in the root and has an important role in restricting the transport of Na+ from the root to the leaves in bread wheat. Thus, TaHKT1;5-D confers the essential salinity tolerance mechanism in bread wheat associated with the Kna1 locus via shoot Na+ exclusion and is critical in maintaining a high K+/Na+ ratio in the leaves. These findings show there is potential to increase the salinity tolerance of bread wheat by manipulation of HKT1;5 genes.en
dc.publisherWiley-Blackwellen
dc.relation.urlhttp://doi.wiley.com/10.1111/tpj.12651en
dc.rightsThis is the peer reviewed version of the following article: Byrt, C. S., Xu, B., Krishnan, M., Lightfoot, D. J., Athman, A., Jacobs, A. K., Watson-Haigh, N. S., Plett, D., Munns, R., Tester, M. and Gilliham, M. (2014), The Na+ transporter, TaHKT1;5-D, limits shoot Na+ accumulation in bread wheat. The Plant Journal, 80: 516–526. doi: 10.1111/tpj.12651, which has been published in final form at http://doi.wiley.com/10.1111/tpj.12651. This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.en
dc.subjectwheaten
dc.subjectTriticum aestivumen
dc.subjectsodium exclusionen
dc.subjectHKTen
dc.subjectsalt toleranceen
dc.subjectRNA interferenceen
dc.titleThe Na+ transporter, TaHKT1;5-D, limits shoot Na+ accumulation in bread wheaten
dc.typeArticleen
dc.contributor.departmentCenter for Desert Agricultureen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalThe Plant Journalen
dc.eprint.versionPost-printen
dc.contributor.institutionCSIRO Plant Industry; Canberra ACT 2601 Australiaen
dc.contributor.institutionSchool of Agriculture; Food and Wine and Waite Research Institute; University of Adelaide; Waite Research Precinct; Glen Osmond SA 5064 Australiaen
dc.contributor.institutionAustralian Centre for Plant Functional Genomics; University of Adelaide; Waite Research Precinct; Glen Osmond SA 5064 Australiaen
dc.contributor.institutionAustralian Research Council Centre of Excellence in Plant Cell Walls, University of Adelaide, Waite Research Precinct, Glen Osmond, SA, Australiaen
dc.contributor.institutionAustralian Research Council Centre of Excellence in Plant Energy Biology, University of Adelaide, Waite Research Precinct, Glen Osmond, SA, Australiaen
dc.contributor.institutionSchool of Information Technology and Mathematical Sciences, University of South Australia, Mawson Lakes, SA, Australiaen
dc.contributor.institutionSchool of Plant Biology and ARC Centre of Excellence in Plant Energy Biology, University of Western Australia, Crawley, WA, Australiaen
kaust.authorLightfoot, Damienen
kaust.authorTester, Mark A.en
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