Maize maintains growth in response to decreased nitrate supply through a highly dynamic and developmental stage-specific transcriptional response

Handle URI:
http://hdl.handle.net/10754/556195
Title:
Maize maintains growth in response to decreased nitrate supply through a highly dynamic and developmental stage-specific transcriptional response
Authors:
Plett, Darren; Baumann, Ute; Schreiber, Andreas W.; Holtham, Luke; Kalashyan, Elena; Toubia, John; Nau, John; Beatty, Mary; Rafalski, Antoni; Dhugga, Kanwarpal S.; Tester, Mark A. ( 0000-0002-5085-8801 ) ; Garnett, Trevor; Kaiser, Brent N.
Abstract:
Elucidation of the gene networks underlying the response to N supply and demand will facilitate the improvement of the N uptake efficiency of plants. We undertook a transcriptomic analysis of maize to identify genes responding to both a non-growth-limiting decrease in NO3- provision and to development-based N demand changes at seven representative points across the life cycle. Gene co-expression networks were derived by cluster analysis of the transcript profiles. The majority of NO3--responsive transcription occurred at 11 (D11), 18 (D18) and 29 (D29) days after emergence, with differential expression predominating in the root at D11 and D29 and in the leaf at D18. A cluster of 98 probe sets was identified, the expression pattern of which is similar to that of the high-affinity NO3- transporter (NRT2) genes across the life cycle. The cluster is enriched with genes encoding enzymes and proteins of lipid metabolism and transport, respectively. These are candidate genes for the response of maize to N supply and demand. Only a few patterns of differential gene expression were observed over the entire life cycle; however, the composition of the classes of the genes differentially regulated at individual time points was unique, suggesting tightly controlled regulation of NO3--responsive gene expression. © 2015 Society for Experimental Biology, Association of Applied Biologists and John Wiley & Sons Ltd.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division
Citation:
Maize maintains growth in response to decreased nitrate supply through a highly dynamic and developmental stage-specific transcriptional response 2015:n/a Plant Biotechnology Journal
Journal:
Plant Biotechnology Journal
Issue Date:
2-Jun-2015
DOI:
10.1111/pbi.12388
Type:
Article
ISSN:
14677644
Additional Links:
http://doi.wiley.com/10.1111/pbi.12388
Appears in Collections:
Articles; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorPlett, Darrenen
dc.contributor.authorBaumann, Uteen
dc.contributor.authorSchreiber, Andreas W.en
dc.contributor.authorHoltham, Lukeen
dc.contributor.authorKalashyan, Elenaen
dc.contributor.authorToubia, Johnen
dc.contributor.authorNau, Johnen
dc.contributor.authorBeatty, Maryen
dc.contributor.authorRafalski, Antonien
dc.contributor.authorDhugga, Kanwarpal S.en
dc.contributor.authorTester, Mark A.en
dc.contributor.authorGarnett, Trevoren
dc.contributor.authorKaiser, Brent N.en
dc.date.accessioned2015-06-03T08:35:55Zen
dc.date.available2015-06-03T08:35:55Zen
dc.date.issued2015-06-02en
dc.identifier.citationMaize maintains growth in response to decreased nitrate supply through a highly dynamic and developmental stage-specific transcriptional response 2015:n/a Plant Biotechnology Journalen
dc.identifier.issn14677644en
dc.identifier.doi10.1111/pbi.12388en
dc.identifier.urihttp://hdl.handle.net/10754/556195en
dc.description.abstractElucidation of the gene networks underlying the response to N supply and demand will facilitate the improvement of the N uptake efficiency of plants. We undertook a transcriptomic analysis of maize to identify genes responding to both a non-growth-limiting decrease in NO3- provision and to development-based N demand changes at seven representative points across the life cycle. Gene co-expression networks were derived by cluster analysis of the transcript profiles. The majority of NO3--responsive transcription occurred at 11 (D11), 18 (D18) and 29 (D29) days after emergence, with differential expression predominating in the root at D11 and D29 and in the leaf at D18. A cluster of 98 probe sets was identified, the expression pattern of which is similar to that of the high-affinity NO3- transporter (NRT2) genes across the life cycle. The cluster is enriched with genes encoding enzymes and proteins of lipid metabolism and transport, respectively. These are candidate genes for the response of maize to N supply and demand. Only a few patterns of differential gene expression were observed over the entire life cycle; however, the composition of the classes of the genes differentially regulated at individual time points was unique, suggesting tightly controlled regulation of NO3--responsive gene expression. © 2015 Society for Experimental Biology, Association of Applied Biologists and John Wiley & Sons Ltd.en
dc.relation.urlhttp://doi.wiley.com/10.1111/pbi.12388en
dc.rightsThis is the peer reviewed version of the following article: Plett, D., Baumann, U., Schreiber, A.W., Holtham, L., Kalashyan, E., Toubia, J., Nau, J., Beatty, M., Rafalski, A., Dhugga, K.S., Tester, M., Garnett, T. and Kaiser, B.N. (2015) Maize maintains growth in response to decreased nitrate supply through a highly dynamic and developmental stage-specific transcriptional response. Plant Biotechnol. J., doi: 10.1111/pbi.12388, which has been published in final form at http://doi.wiley.com/10.1111/pbi.12388. This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.en
dc.subjectN use efficiencyen
dc.subjecthigh-affinity nitrate transporteren
dc.subjectNRT2en
dc.subjectmicroarrayen
dc.subjectgene cluster analysisen
dc.subjectlipid metabolismen
dc.titleMaize maintains growth in response to decreased nitrate supply through a highly dynamic and developmental stage-specific transcriptional responseen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalPlant Biotechnology Journalen
dc.eprint.versionPost-printen
dc.contributor.institutionAustralian Centre for Plant Functional Genomics; Waite Research Institute; University of Adelaide; Adelaide SA Australiaen
dc.contributor.institutionAustralian Centre for Plant Functional Genomics; Waite Research Institute; University of Adelaide; Adelaide SA Australiaen
dc.contributor.institutionAustralian Centre for Plant Functional Genomics; Waite Research Institute; University of Adelaide; Adelaide SA Australiaen
dc.contributor.institutionAustralian Centre for Plant Functional Genomics; Waite Research Institute; University of Adelaide; Adelaide SA Australiaen
dc.contributor.institutionAustralian Centre for Plant Functional Genomics; Waite Research Institute; University of Adelaide; Adelaide SA Australiaen
dc.contributor.institutionAustralian Centre for Plant Functional Genomics; Waite Research Institute; University of Adelaide; Adelaide SA Australiaen
dc.contributor.institutionDuPont Pioneer; Johnston IA USAen
dc.contributor.institutionDuPont Pioneer; Johnston IA USAen
dc.contributor.institutionDuPont Pioneer; Wilmington DE USAen
dc.contributor.institutionDuPont Pioneer; Johnston IA USAen
dc.contributor.institutionAustralian Centre for Plant Functional Genomics; Waite Research Institute; University of Adelaide; Adelaide SA Australiaen
dc.contributor.institutionSchool of Agriculture, Food and Wine; Waite Research Institute; University of Adelaide; Adelaide SA Australiaen
dc.contributor.institutionACRF South Australian Cancer Genomics Facility, Centre for Cancer Biology, SA Pathology, Adelaide, SA, Australiaen
dc.contributor.institutionSchool of Molecular and Biomedical Science, The University of Adelaide, Adelaide, SA, Australiaen
dc.contributor.institutionThe Plant Accelerator, Australian Plant Phenomics Facility, The University of Adelaide, PMB 1, Glen Osmond, SA, Australiaen
dc.contributor.institutionCentre for Carbon Water and Food, The Faculty of Agriculture and Environment, The University of Sydney, Camden, NSW, Australiaen
kaust.authorTester, Mark A.en
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